Browsing by Author "Uehara, Anna"

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    Emergence of Epidemic Dengue-1 Virus in the Southern Province of Sri Lanka.
    (PLoS Negl Trop Dis, 2016-10) Bodinayake, Champica K; Tillekeratne, L Gayani; Nagahawatte, Ajith; Devasiri, Vasantha; Kodikara Arachichi, Wasantha; Strouse, John J; Sessions, October M; Kurukulasooriya, Ruvini; Uehara, Anna; Howe, Shiqin; Ong, Xin Mei; Tan, Sharon; Chow, Angelia; Tummalapalli, Praveen; De Silva, Aruna D; Østbye, Truls; Woods, Christopher W; Gubler, Duane J; Reller, Megan E
    BACKGROUND: Dengue is a frequent cause of acute febrile illness with an expanding global distribution. Since the 1960s, dengue in Sri Lanka has been documented primarily along the heavily urbanized western coast with periodic shifting of serotypes. Outbreaks from 2005-2008 were attributed to a new clade of DENV-3 and more recently to a newly introduced genotype of DENV-1. In 2007, we conducted etiologic surveillance of acute febrile illness in the Southern Province and confirmed dengue in only 6.3% of febrile patients, with no cases of DENV-1 identified. To re-evaluate the importance of dengue as an etiology of acute febrile illness in this region, we renewed fever surveillance in the Southern Province to newly identify and characterize dengue. METHODOLOGY/PRINCIPAL FINDINGS: A cross-sectional surveillance study was conducted at the largest tertiary care hospital in the Southern Province from 2012-2013. A total of 976 patients hospitalized with acute undifferentiated fever were enrolled, with 64.3% male and 31.4% children. Convalescent blood samples were collected from 877 (89.6%). Dengue virus isolation, dengue RT-PCR, and paired IgG ELISA were performed. Acute dengue was confirmed as the etiology for 388 (39.8%) of 976 hospitalizations, with most cases (291, 75.0%) confirmed virologically and by multiple methods. Among 351 cases of virologically confirmed dengue, 320 (91.2%) were due to DENV-1. Acute dengue was associated with self-reported rural residence, travel, and months having greatest rainfall. Sequencing of selected dengue viruses revealed that sequences were most closely related to those described from China and Southeast Asia, not nearby India. CONCLUSIONS/SIGNIFICANCE: We describe the first epidemic of DENV-1 in the Southern Province of Sri Lanka in a population known to be susceptible to this serotype because of prior study. Dengue accounted for 40% of acute febrile illnesses in the current study. The emergence of DENV-1 as the foremost serotype in this densely populated but agrarian population highlights the changing epidemiology of dengue and the need for continued surveillance and prevention.
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    Molecular and Epidemiological Assessment of Dengue Fever in Southern Sri Lanka in 2012
    (2014) Uehara, Anna

    The mosquito-borne dengue viruses (DENV 1, 2, 3, and 4) have a rapidly expanding geographic range and have become endemic in over 100 countries with tropical and subtropical climates, including Sri Lanka. In Sri Lanka, dengue outbreaks have occurred primarily in Colombo, the capital, since the 1960s; however, recent reports suggest transmission throughout the island, with a second focus in the port city of Galle in the Southern Province. To better assess the emergence of dengue among fever-causing agents in the Southern Province of Sri Lanka, we collected epidemiological and clinical characteristics, as well as acute and convalescent sera, from febrile patients of 1 year of age or older without a defined source between June 1, 2012 and December 31, 2012 and compared with similar data collected in 2007. We performed whole genome sequencing on representative dengue isolates from Colombo and Galle to assess relatedness. Through serological testing for DENV IgM and IgG antibodies, virus isolation, and molecular testing, we confirmed acute dengue in 64.83% of the febrile population which was composed of DENV1 (93.8%) and DENV4 (6.2 %) serotypes. We did not detect DENV1 in 2007, nor did we detect DENV2 or DENV3 in 2012. Phylogenetic analysis of whole genome sequence from representative samples from 2012 demonstrated that most DENV 1 strains (25 of 26 tested) belonged to genotype 1 which was closely related to strains previously reported in Colombo during the 2009-2010 dengue season and that all DENV4 strains belong to genotype 1 These results support the spread of dengue virus strains from Colombo to southern Sri Lanka, but the timing and directionality of that movement remain unknown.