Browsing by Author "Vidigal, Pedro Marcus Pereira"

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    Bacteriophages that infect Gram-negative bacteria as source of signal-arrest-release motif lysins
    (Research in Microbiology, 2021-03) Gontijo, Marco Túlio Pardini; Vidigal, Pedro Marcus Pereira; Lopez, Maryoris Elisa Soto; Brocchi, Marcelo
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    Expanding the Database of Signal-Anchor-Release Domain Endolysins Through Metagenomics
    (Probiotics and Antimicrobial Proteins, 2022-08) Gontijo, Marco Túlio Pardini; Teles, Mateus Pereira; Vidigal, Pedro Marcus Pereira; Brocchi, Marcelo
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    Genome Sequence of the Enterohemorrhagic Escherichia coli Bacteriophage UFV-AREG1
    (Genome Announcements, 2016-10-27) Lopez, Maryoris E Soto; Batalha, Laís Silva; Vidigal, Pedro Marcus Pereira; Albino, Luiz Augusto A; Boggione, Delaine Meireles Gouveia; Gontijo, Marco Tulio Pardini; Bazzolli, Denise M Soares; Mendonca, Regina C Santos
    ABSTRACT Here, we present the genome sequence of the Escherichia coli bacteriophage UFV-AREG1. This phage was isolated from cowshed wastewater and showed specificity for enterohemorrhagic E. coli O157:H7 (ATCC 43895), E. coli 0111 (CDC O11ab) and E. coli (ATCC 23229).
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    Genomic Characterisation of UFJF_PfDIW6: A Novel Lytic Pseudomonas fluorescens-Phage with Potential for Biocontrol in the Dairy Industry
    (Viruses) Hungaro, Humberto Moreira; Vidigal, Pedro Marcus Pereira; do Nascimento, Edilane Cristina; Gomes da Costa Oliveira, Felipe; Gontijo, Marco Túlio Pardini; Lopez, Maryoris Elisa Soto
    In this study, we have presented the genomic characterisation of UFJF_PfDIW6, a novel lytic Pseudomonas fluorescens-phage with potential for biocontrol in the dairy industry. This phage showed a short linear double-stranded DNA genome (~42 kb) with a GC content of 58.3% and more than 50% of the genes encoding proteins with unknown functions. Nevertheless, UFJF_PfDIW6’s genome was organised into five functional modules: DNA packaging, structural proteins, DNA metabolism, lysogenic, and host lysis. Comparative genome analysis revealed that the UFJF_PfDIW6’s genome is distinct from other viral genomes available at NCBI databases, displaying maximum coverages of 5% among all alignments. Curiously, this phage showed higher sequence coverages (38–49%) when aligned with uncharacterised prophages integrated into Pseudomonas genomes. Phages compared in this study share conserved locally collinear blocks comprising genes of the modules’ DNA packing and structural proteins but were primarily differentiated by the composition of the DNA metabolism and lysogeny modules. Strategies for taxonomy assignment showed that UFJF_PfDIW6 was clustered into an unclassified genus in the Podoviridae clade. Therefore, our findings indicate that this phage could represent a novel genus belonging to the Podoviridae family.
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    Phylogenetic distribution of the bacteriocin repertoire of lactic acid bacteria species associated with artisanal cheese
    (Food Research International, 2020-02) Gontijo, Marco Túlio Pardini; Silva, Jackson de Sousa; Vidigal, Pedro Marcus Pereira; Martin, José Guilherme Prado