Browsing by Author "Wei, Ruhan"
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Item Open Access Approaches to solving gadolinium-based interference in selenium measurementWei, Ruhan; Colón-Franco, JessicaItem Open Access Autoverification-based algorithms to detect preanalytical errors: Two examples(Clinical Biochemistry, 2022-06) Wei, Ruhan; Légaré, William; McShane, Adam JItem Open Access Comparison of three serum androstenedione assays: the Siemens Immulite immunoassay, the Roche Elecsys immunoassay, and an LC-MS/MS assay(2021-10-28) Wei, Ruhan; Kathleen, Bowers; Kroner, Grace; Payto, Drew; Colón-Franco, JessicaItem Open Access Cross-Method Comparison of Serum Androstenedione Measurement Using Three Different Assays: The Siemens Immulite Immunoassay, the Roche Elecsys Immunoassay, and an LC/MS-MS AssayWei, Ruhan; Bowers, Kathleen; Kroner, Grace; Payto, Drew; Colon Franco, JessicaItem Open Access Evaluation of Age and Sex Differences in Contemporary versus High-Sensitivity Troponin I Measurement in Hospitalized Patients.(Journal of clinical medicine, 2024-04) Alkhalaileh, Hussam; Wei, Ruhan; Cordero Rivera, Ashly; Goksel, Mustafa; Lee, Jason KY; Mazzaferri, Ernest; Jones, JoAnna; Li, JieliBackground: With the transition from the contemporary (cTnI) to high-sensitivity troponin assay (hs-cTnI), concerns have arisen regarding the diagnostic differences between these two assays due to analytical distinctions. This study aims to evaluate the age and sex differences between these two assays, as well as the differences resulting from using two different 99th percentile values of the high-sensitivity troponin assay. Method: A retrospective observational study was conducted at an academic medical center, encompassing a total of 449 lithium heparin plasma samples included in the dataset. Both contemporary and high-sensitivity troponin were simultaneously measured using Siemens ADVIA Centaur analyzers. Two sets of sex-specific 99th percentile URLs from the Siemens study (cutoff-1) and Universal Sample Bank data (cutoff-2) were used for the data analysis. Results: The use of cutoff-1 or cutoff-2 had a negligible impact on troponin classification. Troponin elevation significantly increased in individuals > 50 years old for males and >40 years old for females, with both troponin assays. A receiver operating characteristic analysis did not find significant differences between the two assays. The Kaplan-Meier curves showed no differences in survival in cTnI according to the non-sex-specific 99th URL or hs-cTnI (cutoff-2) but showed a slight difference in survival in hs-cTnI (cutoff-1). Conclusions: Overall, there were no significant differences in age and sex in the diagnostic performance between the contemporary and high-sensitivity troponin assays. Selection criteria for the establishment of the 99th percentile URL should be standardized to avoid the misinterpretation of the troponin results.Item Open Access Evaluation of the Clinical Performance of 7 Serological Assays for SARS-CoV-2 for Use in Clinical Laboratories(The Journal of Applied Laboratory Medicine, 2021-07-07) Plaga, Alexis; Wei, Ruhan; Olson, Elizabeth; Payto, Drew; Harrington, John; Nwe-Kissig, Pyik That; Strizzi, Michelle; Zilka, Sarah; Ko, Jennifer; Colón-Franco, Jessica MAbstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serological assays have emerged as a response to the global pandemic, warranting studies evaluating their clinical performance. This study investigated 7 commercially available SARS-CoV-2 serological assays in samples from noninfected individuals and hospitalized patients. Methods SARS-CoV-2 qualitative serological assays by Abbott (IgG), Beckman (IgG), DiaSorin (IgG), EUROIMMUN (IgG and IgA), Roche and Bio-Rad (Total) were evaluated using specimens collected pre-December 2019 (n = 393), from nucleic acid amplification testing (NAAT) negative patients (n = 40), and from 53 patients with COVID-19 by NAAT collected 3–21 days post-onset of symptoms (POS) (N = 83). Negative agreement (NA), positive agreement (PA), and positive and negative predictive values (PPV and NPV) at prevalences of 5% and 10% were calculated. Results The overall %NA; 95% CI in the negative samples were: Roche 99.8%; 99.3–100.2, Beckman 99.8%; 98.7–100.0, Abbott and Bio-Rad 99.3%; 98.0–99.9, DiaSorin 98.4; 97.2–99.6, EUROIMMUN IgG 97.5%; 95.5–98.7, and EUROIMMUN IgA 79.7%; 75.9–83.5), accounting for positive/equivocal results as false positives. The %PA; 95% CI in samples collected 14+ days POS (n = 24) were: Bio-Rad 83.3%; 68.4–98.2, Abbott and Roche 79.2%; 62.9–95.4, EUROIMMUN IgA 70.8%; 52.6–89.0, Beckman 58.3%; 38.6–78.1, DiaSorin 54.2; 34.2–74.1, and EUROIMMUN IgG 50.0%; 30.0–70.0, accounting for negative/equivocal results as false negatives. NPVs ranged from 97.4%–98.9% and 94.7%–97.7% for prevalences 5% and 10%, respectively. PPVs ranged from 15.5%–94.8% and 27.9%–97.4% for prevalences 5% and 10%, respectively. Conclusion The Roche and Beckman assays resulted in fewer false positives, followed by the Bio-Rad and Abbott assays. While the Bio-Rad assay demonstrated higher antibody detection in COVID-19-positive patients, PA claims cannot be established with a high level of confidence in our sample population.Item Open Access Hemolysis Threshold for Potassium Specimens: How Low Should We Go?(The Journal of Applied Laboratory Medicine, 2021-09-01) Wei, Ruhan; Johnson, Jade; Boyert, Nicole; McShane, Adam JItem Open Access RNase L Is Involved in Liposaccharide-Induced Lung Inflammation(Viruses) Wei, Ruhan; Chen, Guanmin; Algehainy, Naseh; Zeng, Chun; Liu, Chunfang; Liu, Hongli; Liu, Wendy; Stacey, Dennis; Zhou, AiminRNase L mediates interferon (IFN) function during viral infection and cell proliferation. Furthermore, the role of RNase L in the regulation of gene expression, cell apoptosis, autophagy, and innate immunity has been well established in the last decade. Tissue distribution reveals that RNase L is highly expressed in the lung and other organs. However, the physiological roles of RNase L in the lung are largely unknown. In this study, we found that polysaccharide (LPS)-induced acute lung injury (ALI) was remarkably intensified in mice deficient in RNase L compared to wild type mice under the same condition. Furthermore, we found that RNase L mediated the TLR4 signaling pathway, and regulated the expression of various pro- and anti-inflammatory genes in the lung tissue and blood. Most importantly, RNase L function in macrophages during LPS stimulation may be independent of the 2-5A system. These findings demonstrate a novel role of RNase L in the immune response via an atypical molecular mechanism.