Browsing by Author "Zheng, M"
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Item Open Access Dual modulation of cell survival and cell death by beta(2)-adrenergic signaling in adult mouse cardiac myocytes.(Proc Natl Acad Sci U S A, 2001-02-13) Zhu, WZ; Zheng, M; Koch, WJ; Lefkowitz, RJ; Kobilka, BK; Xiao, RPThe goal of this study was to determine whether beta(1)-adrenergic receptor (AR) and beta(2)-AR differ in regulating cardiomyocyte survival and apoptosis and, if so, to explore underlying mechanisms. One potential mechanism is that cardiac beta(2)-AR can activate both G(s) and G(i) proteins, whereas cardiac beta(1)-AR couples only to G(s). To avoid complicated crosstalk between beta-AR subtypes, we expressed beta(1)-AR or beta(2)-AR individually in adult beta(1)/beta(2)-AR double knockout mouse cardiac myocytes by using adenoviral gene transfer. Stimulation of beta(1)-AR, but not beta(2)-AR, markedly induced myocyte apoptosis, as indicated by increased terminal deoxynucleotidyltransferase-mediated UTP end labeling or Hoechst staining positive cells and DNA fragmentation. In contrast, beta(2)-AR (but not beta(1)-AR) stimulation elevated the activity of Akt, a powerful survival signal; this effect was fully abolished by inhibiting G(i), G(beta gamma), or phosphoinositide 3 kinase (PI3K) with pertussis toxin, beta ARK-ct (a peptide inhibitor of G(beta gamma)), or LY294002, respectively. This indicates that beta(2)-AR activates Akt via a G(i)-G(beta gamma)-PI3K pathway. More importantly, inhibition of the G(i)-G(beta gamma)-PI3K-Akt pathway converts beta(2)-AR signaling from survival to apoptotic. Thus, stimulation of a single class of receptors, beta(2)-ARs, elicits concurrent apoptotic and survival signals in cardiac myocytes. The survival effect appears to predominate and is mediated by the G(i)-G(beta gamma)-PI3K-Akt signaling pathway.Item Open Access Packings of 3D stars: stability and structure(Granular Matter, 2016-05-01) Zhao, Y; Liu, K; Zheng, M; Barés, J; Dierichs, K; Menges, A; Behringer, RP© 2016, Springer-Verlag Berlin Heidelberg.We describe a series of experiments involving the creation of cylindrical packings of star-shaped particles, and an exploration of the stability of these packings. The stars cover a broad range of arm sizes and frictional properties. We carried out three different kinds of experiments, all of which involve columns that are prepared by raining star particles one-by-one into hollow cylinders. As an additional part of the protocol, we sometimes vibrated the column before removing the confining cylinder. We rate stability in terms of r, the ratio of the mass of particles that fall off a pile when it collapsed, to the total particle mass. The first experiment involved the intrinsic stability of the column when the confining cylinder was removed. The second kind of experiment involved adding a uniform load to the top of the column, and then determining the collapse properties. A third experiment involved testing stability to tipping of the piles. We find a stability diagram relating the pile height, h, versus pile diameter, (Formula presented.) , where the stable and unstable regimes are separated by a boundary that is roughly a power-law in h versus (Formula presented.) with an exponent that is less than unity. Increasing vibration and friction, particularly the latter, both tend to stabilize piles, while increasing particle size can destabilize the system under certain conditions.