Browsing by Subject "(170.3880) Medical and biological imaging"

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    High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing.
    (Biomed Opt Express, 2011-09-01) Zhang, X; Badea, C; Hood, G; Wetzel, A; Qi, Y; Stiles, J; Johnson, GA
    We present a method for high-resolution reconstruction of fluorescent images of the mouse thorax. It features an anatomically guided sampling method to retrospectively eliminate problematic data and a parallel Monte Carlo software package to compute the Jacobian matrix for the inverse problem. The proposed method was capable of resolving microliter-sized femtomole amount of quantum dot inclusions closely located in the middle of the mouse thorax. The reconstruction was verified against co-registered micro-CT data. Using the proposed method, the new system achieved significantly higher resolution and sensitivity compared to our previous system consisting of the same hardware. This method can be applied to any system utilizing similar imaging principles to improve imaging performance.
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    ItemOpen Access
    In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature.
    (Biomed Opt Express, 2011-06-01) Matthews, Thomas E; Wilson, Jesse W; Degan, Simone; Simpson, Mary Jane; Jin, Jane Y; Zhang, Jennifer Y; Warren, Warren S
    We performed epi-mode pump-probe imaging of melanin in excised human pigmented lesions and both hemoglobin and melanin in live xenograft mouse melanoma models to depths greater than 100 µm. Eumelanin and pheomelanin images, which have been previously demonstrated to differentiate melanoma from benign lesions, were acquired at the dermal-epidermal junction with cellular resolution and modest optical powers (down to 15 mW). We imaged dermal microvasculature with the same wavelengths, allowing simultaneous acquisition of melanin, hemoglobin and multiphoton autofluorescence images. Molecular pump-probe imaging of melanocytes, skin structure and microvessels allows comprehensive, non-invasive characterization of pigmented lesions.