Browsing by Subject "Biotechnology & Applied Microbiology"
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Item Open Access A new non-enzymatic method for isolating human intervertebral disc cells preserves the phenotype of nucleus pulposus cells.(Cytotechnology, 2014-12) Tang, Xinyan; Richardson, William J; Fitch, Robert D; Brown, Christopher R; Isaacs, Robert E; Chen, JunCells isolated from intervertebral disc (IVD) tissues of human surgical samples are one of potential sources for the IVD cellular therapy. The purpose of this study was to develop a new non-enzymatic method, "tissue incubation", for isolating human IVD cells. The IVD tissues of annulus fibrosus (AF) and nucleus pulposus (NP) were incubated separately in tissue culture flasks with culture medium. After 7-10 days incubation, cells were able to migrate out of IVD tissues and proliferate in vitro. After 3-4 weeks culture, expanded cells were harvested by trypsinization, and the remaining tissues were transferred to a new flask for another round of incubation. The molecular phenotype of IVD cells from juvenile and adult human samples was evaluated by both flow cytometry analysis and immunocytochemical staining for the expression of protein markers of NP cells (CD24, CD54, CD239, integrin α6 and laminin α5). Flow cytometry confirmed that both AF and NP cells of all ages positively expressed CD54 and integrin α6, with higher expression levels in NP cells than in AF cells for the juvenile group sample. However, CD24 expression was only found in juvenile NP cells, and not in AF or older disc cells. Similar expression patterns for NP markers were also confirmed by immunocytochemistry. In summary, this new non-enzymatic tissue incubation method for cell isolation preserves molecular phenotypic markers of NP cells and may provide a valuable cell source for the study of NP regeneration strategies.Item Open Access Cord blood derived cell therapy product, DUOC-01, accelerates remyelination in a murine model of cuprizone induced demyelination(Cytotherapy, 2015-06) Saha, Arjun; Buntz, Susan; Patel, Sachit; Bentz, Marcia; Snyder, David; Ozamiz, April; Rusche, Benjamin; Gentry, Tracy; Glenn, Matsushima; Kurtzberg, Joanne; Balber, AndrewItem Open Access DUOC-01, A CANDIDATE CELL THERAPY PRODUCT DERIVED FROM BANKED CORD BLOOD, ACCELERATES BRAIN REMYELINATION IN NSG MICE FOLLOWING CUPRIZONE FEEDING(CYTOTHERAPY, 2014-04-01) Saha, A; Buntz, S; Patel, S; Matsushima, GK; Wollish, A; Kurtzberg, J; Balber, AItem Open Access Effect of Lifestyle Changes after Percutaneous Coronary Intervention on Revascularization.(BioMed research international, 2020-01) Wang, Yang; Xian, Ying; Chen, Tao; Zhao, Yanyan; Yang, Jinggang; Xu, Bo; Li, WeiObjective:Whether optimal cardiovascular health metrics may reduce the risk of cardiovascular events in secondary prevention is uncertain. The study was conducted to evaluate the influence of lifestyle changes on clinical outcomes among the subjects underwent percutaneous coronary intervention (PCI). Methods:The study group consists of 17,099 consecutive PCI patients. We recorded data on subject lifestyle behavior changes after their procedure. Patients were categorized as ideal, intermediate, or poor CV health according to a modified Life's Simple 7 score (on body mass, smoking, physical activity, diet, cholesterol, blood pressure, and glucose). Multivariable COX regression was used to evaluate the association between CV health and revascularization event. We also tested the impact of cumulative cardiovascular health score on reoccurrence of cardiovascular event. Results:During a 3-year median follow-up, 1,583 revascularization events were identified. The observed revascularization rate was 8.0%, 9.3%, and 10.6% in the group of patients with optimal (a modified Life's Simple 7 score of 11-14), average (score = 9 or 10), or inadequate (less or equal than 8) CV health, respectively. After multivariable analysis, the adjusted hazard ratios were 0.83 (95% CI: 0.73-0.94) and 0.89 (95% CI: 0.79-0.99) for patients with optimal and average lifestyle changes comparing with the inadequate tertile (P for trend = 0.003). In addition, each unit increase in above metrics was associated with a decrease risk of revascularization (HR, 0.96; 95% confidence interval, 0.93-0.98; P for trend = 0.003). In addition, each unit increase in above metrics was associated with a decrease risk of revascularization (HR, 0.96; 95% confidence interval, 0.93-0.98. Conclusion:Ideal CV health related to lower incidence of cardiovascular events, even after the percutaneous coronary intervention. Revascularization can be reduced by lifestyle changes. The cardiovascular health metrics could be extrapolated to secondary prevention and need for further validation.Item Open Access Human cord blood derived CD14 cell therapy provides neuroprotection in aquired brain injury(Cytotherapy, 2015-06) Patel, Sachit; Saha, Arjun; Buntz, Susan; Bentz, Marcia; Scotland, Paula; Storms, Robert; Ramamurthy, Poorna; Kurtzberg, Joanne; Balber, AndrewItem Open Access Neural engraftment of a cord blood-derived cell product following intrathecal transplantation(Cytotherapy, 2015-06) Storms, Robert; Lew, Joanna; Liu, Congxiao; Gentry, Tracy; Ozamiz, April; Rusche, Benjamin; Balber, Andrew; Kurtzberg, JoanneItem Open Access Neuronal and glial cell composition in a mouse brain slice culture model is useful in developing human cord blood derived cellular therapies for neonatal hypoxic-ischemic brain injury(Cytotherapy, 2014-04) Patel, S; Saha, A; Buntz, S; Kurtzberg, J; Balber, AItem Open Access Preclinical characterization of DUOC-01, a candidate cell therapy product derived from human banked umbilical cord blood intended for use in treatment of demyelinating diseases(Cytotherapy, 2014-04) Kurtzberg, J; Buntz, S; Gentry, T; Storms, R; Wenger, DA; Noldner, P; Zhou, J; Ozamiz, A; Rusche, B; Balber, AItem Open Access PROTECTION OF BRAIN CELLS IN ORGANOTYPIC SLICE CULTURES EXPOSED TO OXYGEN AND GLUCOSE DEPRIVATION IS SPECIFICALLY MEDIATED BY CORD BLOOD CD14+CELLS(CYTOTHERAPY, 2014-04-01) Saha, A; Buntz, S; Kurtzberg, J; Balber, AItem Open Access Reused Cultivation Water Accumulates Dissolved Organic Carbon and Uniquely Influences Different Marine Microalgae(Frontiers in Bioengineering and Biotechnology, 2019-05-14) Loftus, Sarah E; Johnson, Zackary IItem Open Access The use of hydroxyethyl starch (HES) as a substitute for dextran 40 in the thaw and washing of HPC, apheresis products(Cytotherapy, 2015-06) Zhu, Fenlu; Heidtke, Sarah M; Kurtzberg, Joanne; Hari, Parameswaran; Margolis, David A; Keever-Taylor, CarolynItem Open Access Tissue distribution of a cord blood-derived cell product following intrathecal transplantation(Cytotherapy, 2014-04) Storms, R; Liu, C; Gentry, T; Zhou, J; Ozamiz, A; Rusche, B; Balber, A; Kurtzberg, J