Browsing by Subject "Cell Biology"
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Item Open Access A new non-enzymatic method for isolating human intervertebral disc cells preserves the phenotype of nucleus pulposus cells.(Cytotechnology, 2014-12) Tang, Xinyan; Richardson, William J; Fitch, Robert D; Brown, Christopher R; Isaacs, Robert E; Chen, JunCells isolated from intervertebral disc (IVD) tissues of human surgical samples are one of potential sources for the IVD cellular therapy. The purpose of this study was to develop a new non-enzymatic method, "tissue incubation", for isolating human IVD cells. The IVD tissues of annulus fibrosus (AF) and nucleus pulposus (NP) were incubated separately in tissue culture flasks with culture medium. After 7-10 days incubation, cells were able to migrate out of IVD tissues and proliferate in vitro. After 3-4 weeks culture, expanded cells were harvested by trypsinization, and the remaining tissues were transferred to a new flask for another round of incubation. The molecular phenotype of IVD cells from juvenile and adult human samples was evaluated by both flow cytometry analysis and immunocytochemical staining for the expression of protein markers of NP cells (CD24, CD54, CD239, integrin α6 and laminin α5). Flow cytometry confirmed that both AF and NP cells of all ages positively expressed CD54 and integrin α6, with higher expression levels in NP cells than in AF cells for the juvenile group sample. However, CD24 expression was only found in juvenile NP cells, and not in AF or older disc cells. Similar expression patterns for NP markers were also confirmed by immunocytochemistry. In summary, this new non-enzymatic tissue incubation method for cell isolation preserves molecular phenotypic markers of NP cells and may provide a valuable cell source for the study of NP regeneration strategies.Item Open Access Cord blood derived cell therapy product, DUOC-01, accelerates remyelination in a murine model of cuprizone induced demyelination(Cytotherapy, 2015-06) Saha, Arjun; Buntz, Susan; Patel, Sachit; Bentz, Marcia; Snyder, David; Ozamiz, April; Rusche, Benjamin; Gentry, Tracy; Glenn, Matsushima; Kurtzberg, Joanne; Balber, AndrewItem Open Access DUOC-01, A CANDIDATE CELL THERAPY PRODUCT DERIVED FROM BANKED CORD BLOOD, ACCELERATES BRAIN REMYELINATION IN NSG MICE FOLLOWING CUPRIZONE FEEDING(CYTOTHERAPY, 2014-04-01) Saha, A; Buntz, S; Patel, S; Matsushima, GK; Wollish, A; Kurtzberg, J; Balber, AItem Open Access Human cord blood derived CD14 cell therapy provides neuroprotection in aquired brain injury(Cytotherapy, 2015-06) Patel, Sachit; Saha, Arjun; Buntz, Susan; Bentz, Marcia; Scotland, Paula; Storms, Robert; Ramamurthy, Poorna; Kurtzberg, Joanne; Balber, AndrewItem Open Access Integrative Regulatory Mapping Indicates that the RNA-Binding Protein HuR Couples Pre-mRNA Processing and mRNA Stability(MOLECULAR CELL, 2011-08-05) Mukherjee, Neelanjan; Corcoran, David L; Nusbaum, Jeffrey D; Reid, David W; Georgiev, Stoyan; Hafner, Markus; Ascano, Manuel; Tuschl, Thomas; Ohler, Uwe; Keene, Jack DItem Open Access Neural engraftment of a cord blood-derived cell product following intrathecal transplantation(Cytotherapy, 2015-06) Storms, Robert; Lew, Joanna; Liu, Congxiao; Gentry, Tracy; Ozamiz, April; Rusche, Benjamin; Balber, Andrew; Kurtzberg, JoanneItem Open Access Neuronal and glial cell composition in a mouse brain slice culture model is useful in developing human cord blood derived cellular therapies for neonatal hypoxic-ischemic brain injury(Cytotherapy, 2014-04) Patel, S; Saha, A; Buntz, S; Kurtzberg, J; Balber, AItem Open Access Oxidative stress promotes SIRT1 recruitment to the GADD34/PP1α complex to activate its deacetylase function.(Cell death and differentiation, 2018-02) Lee, Irene Chengjie; Ho, Xue Yan; George, Simi Elizabeth; Goh, Catherine Wenhui; Sundaram, Jeyapriya Rajameenakshi; Pang, Karen Ka Lam; Luo, Weiwei; Yusoff, Permeen; Sze, Newman Siu Kwan; Shenolikar, ShirishPhosphorylation of the eukaryotic translation initiation factor, eIF2α, by stress-activated protein kinases and dephosphorylation by the growth arrest and DNA damage-inducible protein (GADD34)-containing phosphatase is a central node in the integrated stress response. Mass spectrometry demonstrated GADD34 acetylation at multiple lysines. Substituting K315 and K322 with alanines or glutamines did not impair GADD34's ability to recruit protein phosphatase 1α (PP1α) or eIF2α, suggesting that GADD34 acetylation did not modulate eIF2α phosphatase activity. Arsenite (Ars)-induced oxidative stress increased cellular GADD34 levels and enhanced Sirtuin 1 (SIRT1) recruitment to assemble a cytoplasmic complex containing GADD34, PP1α, eIF2α and SIRT1. Induction of GADD34 in WT MEFs paralleled the dephosphorylation of eIF2α (phosphoserine-51) and SIRT1 (phosphoserine-47). By comparison, eIF2α and SIRT1 were persistently phosphorylated in Ars-treated GADD34-/- MEFs. Expressing WT GADD34, but not a mutant unable to bind PP1α in GADD34-/- MEFs restored both eIF2α and SIRT1 dephosphorylation. SIRT1 dephosphorylation increased its deacetylase activity, measured in vitro and in cells. Loss of function of GADD34 or SIRT1 enhanced cellular p-eIF2α levels and attenuated cell death following Ars exposure. These results highlighted a novel role for the GADD34/PP1α complex in coordinating the dephosphorylation and reactivation of eIF2α and SIRT1 to determine cell fate following oxidative stress.Item Open Access Preclinical characterization of DUOC-01, a candidate cell therapy product derived from human banked umbilical cord blood intended for use in treatment of demyelinating diseases(Cytotherapy, 2014-04) Kurtzberg, J; Buntz, S; Gentry, T; Storms, R; Wenger, DA; Noldner, P; Zhou, J; Ozamiz, A; Rusche, B; Balber, AItem Open Access PROTECTION OF BRAIN CELLS IN ORGANOTYPIC SLICE CULTURES EXPOSED TO OXYGEN AND GLUCOSE DEPRIVATION IS SPECIFICALLY MEDIATED BY CORD BLOOD CD14+CELLS(CYTOTHERAPY, 2014-04-01) Saha, A; Buntz, S; Kurtzberg, J; Balber, AItem Open Access The use of hydroxyethyl starch (HES) as a substitute for dextran 40 in the thaw and washing of HPC, apheresis products(Cytotherapy, 2015-06) Zhu, Fenlu; Heidtke, Sarah M; Kurtzberg, Joanne; Hari, Parameswaran; Margolis, David A; Keever-Taylor, CarolynItem Open Access Tissue distribution of a cord blood-derived cell product following intrathecal transplantation(Cytotherapy, 2014-04) Storms, R; Liu, C; Gentry, T; Zhou, J; Ozamiz, A; Rusche, B; Balber, A; Kurtzberg, J