Browsing by Subject "Exposure assessment"

Semi-volatile organic compounds (SVOCs) are used in consumer products in a wide variety of applications such as flame retardants, plasticizers, pesticides, preservatives, and fragrances. Due to their extensive use in everyday products, SVOCs are widely detected in indoor environments, and human exposure is common and often chronic. As the wealth of toxicological data examining the negative health impacts of these compounds grows, the need for reliable tools to accurately measure human exposure becomes increasingly more crucial. In the past few decades, external exposure to these compounds have been evaluated through measurements in indoor air, house dust, and hand wipes, all of which have been shown to be associated with internal dose (e.g., concentrations in urine or blood). However, there are significant limitations to using each of these approaches to characterizing exposure. In recent years, silicone wristbands have been used as personal passive samplers for evaluating ambient exposure to a wide array of consumer product and industrial compounds. While over a thousand chemicals have been reported to be detected on the wristbands, very few studies have measured the concentrations on wristbands and determined how well they correlate to established biomarkers of exposure. This dissertation research sought to evaluate the use of silicone wristbands for measuring personal exposure to three classes of SVOCs- organophosphate esters (OPEs), brominated flame retardants (BFRs), and phthalates. The central hypothesis is that wristbands are an effective tool for evaluating personal exposure to SVOCs and provide more accurate measures of exposure compared to tools currently in use.

Within the first aim of this dissertation research, paired samples of polyurethane foam (collected from sofas), house dust, and serum were analyzed for flame retardants (FRs) chemicals and associations were evaluated. The detection of two FR mixtures, PentaBDE and FM 550, in foam was significantly associated with 4 to 6.5 times as high concentrations of their primary components in house dust (p<0.01). These relationships were modified by the size of the sofa footprint within the room and dust-loading rates. PentaBDE in foam was also associated with higher levels of individual PBDE congeners in serum, particularly two of the primary congeners BDE-47 and -153. Participants who lived in a home with a sofa containing PentaBDE had serum BDE-47 levels that were 2.5 times as high as participants whose sofa did not contain PentaBDE (p<0.01). This study was the first to relate a specific FR application in a consumer product with house dust and a known biomarker of exposure.

For the second aim of this research, adult exposure to OPEs and BFRs were evaluated using silicone wristbands. OPEs quantified on the wristbands were significantly associated with metabolites from pooled urine samples, and polybrominated diphenyl ethers (PBDEs) on the wristbands were similarly correlated to PBDE levels in serum (rs=0.4-0.6, p<0.05). Several novel BFR compounds which lack verified biomarkers of exposure were also measured on the wristbands and reported for the first time. These two studies were the first to evaluate FR concentrations on wristbands with known biomarkers and represent two of now four published manuscripts providing evidence that measurements on wristbands are predictive of internal dose.

In the third aim of this research, children’s exposure to OPEs, phthalates, and BFRs were examined using silicone wristbands. The ability of the wristband measurements to predict urinary metabolite levels of OPEs and phthalates was compared to that of hand wipes and house dust. Across the three classes, the children’s wristband concentrations were positively and significantly associated with a number of their corresponding biomarkers in both urine and serum, similar to observations in our adult cohort. For OPEs, phthalates, and PBDEs, the wristbands were found to have similar or an improved utility, compared to hand wipes and dust, for evaluating children’s exposures to these compounds. For instance, one of the OPEs, 4-tertbutylphenyl diphenyl phosphate (4tBPDPP), on wristbands was more strongly correlated to its urinary metabolite, tert-butyl phenyl phenyl phosphate (tb-PPP), compared to that on hand wipes and dust (rs=0.35, p<0.01, compared to rs=0.16 and 0.05 for hand wipes and dust, respectively). For the phthalate benzyl butyl phthalate (BBP), wristbands and hand wipes were similar associated with the urinary metabolite, mono benzyl phthalate (MBzP), but both were stronger than the dust correlation (rs=0.56 for wristbands and hand wipes, p<0.001; rs=0.23 for dust, p<0.05). Similar results were observed among the PBDEs on the three exposure mediums and their serum biomarkers, although the magnitudes of correlation with serum were more similar for wristbands and dust.

Taken together this dissertation research provides some of the first insights on the evaluation of personal exposures to SVOCs using silicone wristbands. It includes six distinct studies evaluating human exposure to sixty-five chemicals from three classes of compounds. Further, this research offers novel contributions to the field of exposure science, evaluating the relationship between wristbands and established biomarkers of exposure and comparing them to the existing tools used in standard exposure assessments. Wristbands have the potential to serve as an inexpensive and non-invasive medium for evaluating human exposure to chemical mixtures, and this work provides support for their use in large-scale research efforts to characterize SVOC exposures. Additional research should continue to assess wristbands for their ability to measure meaningful exposures for additional classes of chemicals, and importantly, identify the pathways of exposure (e.g., dermal absorption, inhalation, etc.) that are captured by the wristbands.

Traditional cooking using biomass is associated with adverse health consequences, local environmental degradation, and regional climate change. Improved stoves (ICS; liquefied petroleum gas (LPG), biogas, electric, efficient biomass) are heralded as a solution, but their adoption and use remains low. In the first chapter, I report on a series of pilot programs that utilized the marketing mix principles of promotion, product, price and place to increase stove sales in rural Inia. We found that when given a choice amongst products, households strongly preferred an electric stove over improved biomass-burning options. Households clearly identified price as a significant barrier to adoption, while provision of discounts (e.g., rebates given if households used the stove) or payments in installments were related to higher purchase. Collectively, these pilots point to the importance of continued and extensive testing of messages, pricing models, and responses to different stove types prior to scale-up. Thus, a one-size-fits-all approach will be unlikely to boost ICS adoption.

In the second and third chapters, I analyze the impact of mainly improved stove use on social, environmental, and health outcomes in rural India- first in a sample of biogas stove users in Odisha, India, and next with households in the Himalayan state of Uttarakhand. In both settings, ICS use was associated with reduced use of firewood, substantial time savings for primary cooks, and significant reduction in exposure to particulate matter and polycyclic aromatic hydrocarbons in household air. I find that ICS users in Odisha spend reduced time in the hospital with acute respiratory infection and reduced diastolic blood pressure, but no relationship with other health measurements.

In the third chapter, I also find significant reduction in exposure to personal air pollution. Using temperature sensors as objective stove use monitors for all stoves and heaters we find that households underreport use of improved and traditional stoves.

These papers provide encouraging evidence of potential for adoption of clean stove and a suite of benefits from clean stove use; however, in order to achieve recommended levels of air pollution additional policies may be needed.