Browsing by Subject "GAP"

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    Creating linked datasets for SME energy-assessment evidence-building: Results from the U.S. Industrial Assessment Center Program
    (Energy Policy, 2017-12-01) Dalzell, NM; Boyd, GA; Reiter, JP
    © 2017 Elsevier Ltd Lack of information is commonly cited as a market failure resulting in an energy-efficiency gap. Government information policies to fill this gap may enable improvements in energy efficiency and social welfare because of the externalities of energy use. The U.S. Department of Energy Industrial Assessment Center (IAC) program is one such policy intervention, providing no-cost assessments to small and medium enterprises (SME). The IAC program has assembled a wealth of data on these assessments, but the database does not include information about participants after the assessment or on non-participants. This study addresses that lack by creating a new linked dataset using the public IAC and non-public data at the Census Bureau. The IAC database excludes detail needed for an exact match, so the study developed a linking methodology to account for uncertainty in the matching process. Based on the linking approach, a difference in difference analysis for SME that received an assessment was done; plants that received an assessment improve their performance over time, relative to industry peers that did not. This new linked dataset is likely to shed even more light on the impact of the IAC and similar programs in advancing energy efficiency.
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    Parallel Actin-Independent Recycling Pathways Polarize Cdc42 in Budding Yeast.
    (Curr Biol, 2016-08-22) Woods, Benjamin; Lai, Helen; Wu, Chi-Fang; Zyla, Trevin R; Savage, Natasha S; Lew, Daniel J
    The highly conserved Rho-family GTPase Cdc42 is an essential regulator of polarity in many different cell types. During polarity establishment, Cdc42 becomes concentrated at a cortical site, where it interacts with downstream effectors to orient the cytoskeleton along the front-back axis. To concentrate Cdc42, loss of Cdc42 by diffusion must be balanced by recycling to the front. In Saccharomyces cerevisiae, the guanine nucleotide dissociation inhibitor (GDI) Rdi1 recycles Cdc42 through the cytoplasm. Loss of Rdi1 slowed but did not eliminate Cdc42 accumulation at the front, suggesting the existence of other recycling pathways. One proposed pathway involves actin-directed trafficking of vesicles carrying Cdc42 to the front. However, we found no role for F-actin in Cdc42 concentration, even in rdi1Δ cells. Instead, Cdc42 was still able to exchange between the membrane and cytoplasm in rdi1Δ cells, albeit at a reduced rate. Membrane-cytoplasm exchange of GDP-Cdc42 was faster than that of GTP-Cdc42, and computational modeling indicated that such exchange would suffice to promote polarization. We also uncovered a novel role for the Cdc42-directed GTPase-activating protein (GAP) Bem2 in Cdc42 polarization. Bem2 was known to act in series with Rdi1 to promote recycling of Cdc42, but we found that rdi1Δ bem2Δ mutants were synthetically lethal, suggesting that they also act in parallel. We suggest that GAP activity cooperates with the GDI to counteract the dissipative effect of a previously unappreciated pathway whereby GTP-Cdc42 escapes from the polarity site through the cytoplasm.