Browsing by Subject "Single-Cell Gene Expression Analysis"

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    Protocol for fast scRNA-seq raw data processing using scKB and non-arbitrary quality control with COPILOT.
    (STAR protocols, 2022-12) Hsu, Che-Wei; Shahan, Rachel; Nolan, Trevor M; Benfey, Philip N; Ohler, Uwe
    We describe a protocol to perform fast and non-arbitrary quality control of single-cell RNA sequencing (scRNA-seq) raw data using scKB and COPILOT. scKB is a wrapper script of kallisto and bustools for accelerated alignment and transcript count matrix generation, which runs significantly faster than the popular tool Cell Ranger. COPILOT then offers non-arbitrary background noise removal by comparing distributions of low-quality and high-quality cells. Together, this protocol streamlines the processing workflow and provides an easy entry for new scRNA-seq users. For complete details on the use and execution of this protocol, please refer to Shahan et al. (2022).