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Browsing by Subject "Bacterial Proteins"
Now showing items 21-40 of 40
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Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division.
(Proceedings of the National Academy of Sciences of the United States of America, 2013-11-11)Bacteriophages take over host resources primarily via the activity of proteins expressed early in infection. One of these proteins, produced by the Escherichia coli phage T7, is gene product (Gp) 0.4. Here, we show that ... -
¹H, ¹³C, and ¹⁵N resonance assignments and secondary structure prediction of the full-length transition state regulator AbrB from Bacillus anthracis.
(Biomolecular NMR assignments, 2012-04)The AbrB protein is a transcription factor that regulates the expression of numerous essential genes during the cells transition phase state. AbrB from Bacillus anthracis is, nototriously, the principal protein responsible ... -
High-resolution crystal structures of Escherichia coli FtsZ bound to GDP and GTP.
(Acta crystallographica. Section F, Structural biology communications, 2020-02-05)Bacterial cytokinesis is mediated by the Z-ring, which is formed by the prokaryotic tubulin homolog FtsZ. Recent data indicate that the Z-ring is composed of small patches of FtsZ protofilaments that travel around the bacterial ... -
Identification of BfmR, a response regulator involved in biofilm development, as a target for a 2-Aminoimidazole-based antibiofilm agent.
(Biochemistry, 2012-12)2-Aminoimidazoles (2AIs) have been documented to disrupt bacterial protection mechanisms, including biofilm formation and genetically encoded antibiotic resistance traits. Using Acinetobacter baumannii, we provide initial ... -
Inside-out Z rings--constriction with and without GTP hydrolysis.
(Molecular microbiology, 2011-07)The bacterial tubulin homologue FtsZ forms a ring-like structure called the Z ring that drives cytokinesis. We showed previously that FtsZ-YFP-mts, which has a short amphipathic helix (mts) on its C terminus that inserts ... -
Liposome division by a simple bacterial division machinery.
(Proceedings of the National Academy of Sciences of the United States of America, 2013-07)We previously reconstituted Z rings in tubular multilamellar liposomes with FtsZ-YFP-mts, where mts is a membrane-targeting amphiphilic helix. These reconstituted Z rings generated a constriction force but did not divide ... -
Long range dynamic effects of point-mutations trap a response regulator in an active conformation.
(FEBS letters, 2010-10)When a point-mutation in a protein elicits a functional change, it is most common to assign this change to local structural perturbations. Here we show that point-mutations, distant from an essential highly dynamic kinase ... -
Negative-stain electron microscopy of inside-out FtsZ rings reconstituted on artificial membrane tubules show ribbons of protofilaments.
(Biophysical journal, 2012-07)FtsZ, the primary cytoskeletal element of the Z ring, which constricts to divide bacteria, assembles into short, one-stranded filaments in vitro. These must be further assembled to make the Z ring in bacteria. Conventional ... -
Osmolyte-induced folding of an intrinsically disordered protein: folding mechanism in the absence of ligand.
(Biochemistry, 2010-06-29)Understanding the interconversion between thermodynamically distinguishable states present in a protein folding pathway provides not only the kinetics and energetics of protein folding but also insights into the functional ... -
Rapid in vitro assembly of Caulobacter crescentus FtsZ protein at pH 6.5 and 7.2.
(The Journal of biological chemistry, 2013-08)FtsZ from most bacteria assembles rapidly in vitro, reaching a steady-state plateau in 5-10 s after addition of GTP. A recent study used a novel dynamic light-scattering technique to assay the assembly of FtsZ from Caulobacter ... -
Risk factors for and estimated incidence of community-associated Clostridium difficile infection, North Carolina, USA.
(Emerg Infect Dis, 2010-02)We determined estimated incidence of and risk factors for community-associated Clostridium difficile infection (CA-CDI) among patients treated at 6 North Carolina hospitals. CA-CDI case-patients were defined as adults (>18 ... -
Stress-induced outer membrane vesicle production by Pseudomonas aeruginosa.
(J Bacteriol, 2013-07)As an opportunistic Gram-negative pathogen, Pseudomonas aeruginosa must be able to adapt and survive changes and stressors in its environment during the course of infection. To aid survival in the hostile host environment, ... -
Structure and DNA-binding traits of the transition state regulator AbrB.
(Structure (London, England : 1993), 2014-11)The AbrB protein from Bacillus subtilis is a DNA-binding global regulator controlling the onset of a vast array of protective functions under stressful conditions. Such functions include biofilm formation, antibiotic production, ... -
Structure of the Francisella response regulator QseB receiver domain, and characterization of QseB inhibition by antibiofilm 2-aminoimidazole-based compounds.
(Molecular microbiology, 2017-10)With antibiotic resistance increasing at alarming rates, targets for new antimicrobial therapies must be identified. A particularly promising target is the bacterial two-component system. Two-component systems allow bacteria ... -
Subacute effects of transgenic Cry1ab Bacillus thuringiensis corn litter on the isopods Trachelipus rathkii and Armadillidium nasatum.
(Environmental toxicology and chemistry / SETAC, 2006-10)Laboratory studies were conducted to investigate the subacute effects of transgenic Cry1Ab corn leaf material containing Bacillus thuringiensis (Bt) protein on the terrestrial isopods Trachelipus rathkii and Armadillidium ... -
SulA inhibits assembly of FtsZ by a simple sequestration mechanism.
(Biochemistry, 2012-04)We have investigated the inhibition by SulA of the assembly of Escherichia coli FtsZ. Using quantitative GTPase and fluorescence assays, we found that SulA inhibition resulted in an increase in the apparent critical concentration ... -
The bacterial effector AvrRxo1 inhibits vitamin B6 biosynthesis to promote infection in rice.
(Plant communications, 2022-05)Xanthomonas oryzae pv. oryzicola (Xoc), which causes rice bacterial leaf streak, invades leaves mainly through stomata, which are often closed as a plant immune response against pathogen invasion. How Xoc overcomes stomatal ... -
The C-terminal linker of Escherichia coli FtsZ functions as an intrinsically disordered peptide.
(Molecular microbiology, 2013-07)The tubulin homologue FtsZ provides the cytoskeletal framework and constriction force for bacterial cell division. FtsZ has an 50-amino-acid (aa) linker between the protofilament-forming globular domain and the C-terminal ... -
The Solution Structures and Interaction of SinR and SinI: Elucidating the Mechanism of Action of the Master Regulator Switch for Biofilm Formation in Bacillus subtilis.
(Journal of molecular biology, 2020-01)Bacteria have developed numerous protection strategies to ensure survival in harsh environments, with perhaps the most robust method being the formation of a protective biofilm. In biofilms, bacterial cells are embedded ... -
The Structure of the Biofilm-controlling Response Regulator BfmR from Acinetobacter baumannii Reveals Details of Its DNA-binding Mechanism.
(Journal of molecular biology, 2018-03)The rise of drug-resistant bacterial infections coupled with decreasing antibiotic efficacy poses a significant challenge to global health care. Acinetobacter baumannii is an insidious, emerging bacterial pathogen responsible ...
