Browsing by Author "Berrong, Mark"
Now showing items 1-3 of 3
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Establishment and maintenance of a PBMC repository for functional cellular studies in support of clinical vaccine trials
Sambor, Anna; Garcia, Ambrosia; Berrong, Mark; Pickeral, Joy; Brown, Sara; Rountree, Wes; Sanchez, Ana; ... (19 authors) (Journal of Immunological Methods, 2014-01-01)A large repository of cryopreserved peripheral blood mononuclear cells (PBMCs) samples was created to provide laboratories testing the specimens from human immunodeficiency virus-1 (HIV-1) vaccine clinical trials the material ... -
The External Quality Assurance Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay
Sanchez, Ana M; Rountree, Wes; Berrong, Mark; Garcia, Ambrosia; Schuetz, Alexandra; Cox, Josephine; Frahm, Nicole; ... (12 authors) (Journal of Immunological Methods, 2014-01-01)The interferon-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay has been developed and used as an end-point assay in clinical trials for infectious diseases and cancer to detect the magnitude of antigen-specific immune ... -
Variability of the IFN-γ ELISpot assay in the context of proficiency testing and bridging studies.
Rountree, Wes; Berrong, Mark; Sanchez, Ana M; Denny, Thomas N; Ferrari, Guido (J Immunol Methods, 2016-06)Assays that assess cellular mediated immune responses performed under Good Clinical Laboratory Practice (GCLP) guidelines are required to provide specific and reproducible results. Defined validation procedures are required ...