Browsing by Author "Berrong, Mark"

Establishment and maintenance of a PBMC repository for functional cellular studies in support of clinical vaccine trials.

Sambor, Anna; Garcia, Ambrosia; Berrong, Mark; Pickeral, Joy; Brown, Sara; Rountree, Wes; Sanchez, Ana; ... (19 authors) (J Immunol Methods, 2014-07)

A large repository of cryopreserved peripheral blood mononuclear cells (PBMCs) samples was created to provide laboratories testing the specimens from human immunodeficiency virus-1 (HIV-1) vaccine clinical trials the material ...

The External Quality Assurance Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay.

Sanchez, Ana M; Rountree, Wes; Berrong, Mark; Garcia, Ambrosia; Schuetz, Alexandra; Cox, Josephine; Frahm, Nicole; ... (12 authors) (Journal of Immunological Methods, 2014-07)

The interferon-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay has been developed and used as an end-point assay in clinical trials for infectious diseases and cancer to detect the magnitude of antigen-specific immune ...

Variability of the IFN-γ ELISpot assay in the context of proficiency testing and bridging studies.

Rountree, Wes; Berrong, Mark; Sanchez, Ana M; Denny, Thomas N; Ferrari, Guido (J Immunol Methods, 2016-06)

Assays that assess cellular mediated immune responses performed under Good Clinical Laboratory Practice (GCLP) guidelines are required to provide specific and reproducible results. Defined validation procedures are required ...