Browsing by Author "Denny, Thomas"
Now showing items 1-15 of 15
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A model for harmonizing flow cytometry in clinical trials.
(Nat Immunol, 2010-11)Complexities in sample handling, instrument setup and data analysis are barriers to the effective use of flow cytometry to monitor immunological parameters in clinical trials. The novel use of a central laboratory may help ... -
Early experience with universal preprocedural testing for SARS-CoV-2 in a relatively low-prevalence area.
(Infection control and hospital epidemiology, 2020-08-03)We implemented universal severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing of patients undergoing surgical procedures as a means to conserve personal protective equipment (PPE). The rate of asymptomatic ... -
Establishment and maintenance of a PBMC repository for functional cellular studies in support of clinical vaccine trials.
(J Immunol Methods, 2014-07)A large repository of cryopreserved peripheral blood mononuclear cells (PBMCs) samples was created to provide laboratories testing the specimens from human immunodeficiency virus-1 (HIV-1) vaccine clinical trials the material ... -
External Quality Assessment Program for Next-Generation Sequencing-Based HIV Drug Resistance Testing: Logistical Considerations.
(Viruses, 2020-05-18)Next-generation sequencing (NGS) is likely to become the new standard method for HIV drug resistance (HIVDR) genotyping. Despite the significant advances in the development of wet-lab protocols and bioinformatic data processing ... -
HIV-1 subtype C is significantly more infectious than other subtypes
(JOURNAL OF THE INTERNATIONAL AIDS SOCIETY, 2015-07) -
Infectious virion capture by HIV-1 gp120-specific IgG from RV144 vaccinees.
(J Virol, 2013-07)The detailed examination of the antibody repertoire from RV144 provides a unique template for understanding potentially protective antibody functions. Some potential immune correlates of protection were untested in the correlates ... -
SARS-CoV-2 Viremia is Associated with COVID-19 Severity and Predicts Clinical Outcomes.
(Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2021-08-10)<h4>Background</h4>SARS-CoV-2 viral RNA (vRNA) is detected in the bloodstream of some patients with COVID-19 ("RNAemia") but it is not clear whether this RNAemia reflects viremia (i.e., virus particles) and how RNAemia/viremia ... -
Systematic review of the performance of HIV viral load technologies on plasma samples.
(PLoS One, 2014)BACKGROUND: Viral load (VL) monitoring is the standard of care in developing country settings for detecting HIV treatment failure. Since 2010 the World Health Organization has recommended a phase-in approach to VL monitoring ... -
Systematic review of the use of dried blood spots for monitoring HIV viral load and for early infant diagnosis.
(PLoS One, 2014)BACKGROUND: Dried blood spots (DBS) have been used as alternative specimens to plasma to increase access to HIV viral load (VL) monitoring and early infant diagnosis (EID) in remote settings. We systematically reviewed evidence ... -
The External Quality Assurance Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay.
(Journal of Immunological Methods, 2014-07)The interferon-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay has been developed and used as an end-point assay in clinical trials for infectious diseases and cancer to detect the magnitude of antigen-specific immune ... -
Vaccine-Induced Antibodies Mediate Higher Antibody-Dependent Cellular Cytotoxicity After Interleukin-15 Pretreatment of Natural Killer Effector Cells.
(Frontiers in immunology, 2019-01)The secondary analyses for correlates of risk of infection in the RV144 HIV-1 vaccine trial implicated vaccine-induced antibody-dependent cellular cytotoxicity (ADCC) responses in the observed protection, highlighting the ...
