Now showing items 1-20 of 39

    • A Unified Model for Treadmilling and Nucleation of Single-Stranded FtsZ Protofilaments. 

      Corbin, Lauren C; Erickson, Harold P (Biophysical journal, 2020-08)
      Bacterial cell division is tightly coupled to the dynamic behavior of FtsZ, a tubulin homolog. Recent experimental work in vitro and in vivo has attributed FtsZ's assembly dynamics to treadmilling, in which subunits add ...
    • Cell division without FtsZ--a variety of redundant mechanisms. 

      Erickson, Harold P; Osawa, Masaki (Molecular microbiology, 2010-10)
      Until 1998 it looked like all bacteria and archaea used a universal cytokinetic machine based on FtsZ. A dozen completely sequenced bacterial genomes all had an ftsZ gene, as did the several sequenced archaeal genomes. Then ...
    • Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings. 

      Osawa, Masaki; Erickson, Harold P (Methods in enzymology, 2009-01)
      We have developed a system for producing tubular multilamellar liposomes that incorporate the protein FtsZ on the inside. We start with a mixture of spherical multilamellar liposomes with FtsZ initially on the outside. Shearing ...
    • Conformational changes of FtsZ reported by tryptophan mutants. 

      Chen, Yaodong; Erickson, Harold P (Biochemistry, 2011-05-03)
      E. coli FtsZ has no native tryptophan. We showed previously that the mutant FtsZ L68W gave a 2.5-fold increase in trp fluorescence when assembly was induced by GTP. L68 is probably buried in the protofilament interface upon ...
    • Curved FtsZ protofilaments generate bending forces on liposome membranes. 

      Osawa, Masaki; Anderson, David E; Erickson, Harold P (The EMBO journal, 2009-11)
      We have created FtsZ-YFP-mts where an amphipathic helix on the C-terminus tethers FtsZ to the membrane. When incorporated inside multi-lamellar tubular liposomes, FtsZ-YFP-mts can assemble Z rings that generate a constriction ...
    • Fibronectin aggregation and assembly: the unfolding of the second fibronectin type III domain. 

      Ohashi, Tomoo; Erickson, Harold P (The Journal of biological chemistry, 2011-11)
      The mechanism of fibronectin (FN) assembly and the self-association sites are still unclear and contradictory, although the N-terminal 70-kDa region ((I)1-9) is commonly accepted as one of the assembly sites. We previously ...
    • Fibronectin Conformation and Assembly: Analysis of Fibronectin Deletion Mutants and Fibronectin Glomerulopathy (GFND) Mutants. 

      Ohashi, Tomoo; Lemmon, Christopher A; Erickson, Harold P (Biochemistry, 2017-08-11)
      To study fibronectin (FN) conformation and assembly, we generated several deletion mutants: FNΔ(I)1-5, FNΔ(III)1-3, FNΔ(III)4-8, and FNΔ(III)11-14. A monomeric form, FNmono, which lacked the C-terminal dimerization region, ...
    • FtsZ filament capping by MciZ, a developmental regulator of bacterial division. 

      Bisson-Filho, Alexandre W; Discola, Karen F; Castellen, Patrícia; Blasios, Valdir; Martins, Alexandre; Sforça, Maurício L; Garcia, Wanius; ... (11 authors) (Proceedings of the National Academy of Sciences of the United States of America, 2015-04-06)
      Cytoskeletal structures are dynamically remodeled with the aid of regulatory proteins. FtsZ (filamentation temperature-sensitive Z) is the bacterial homolog of tubulin that polymerizes into rings localized to cell-division ...
    • FtsZ in bacterial cytokinesis: cytoskeleton and force generator all in one. 

      Erickson, Harold P; Anderson, David E; Osawa, Masaki (Microbiology and molecular biology reviews : MMBR, 2010-12)
      FtsZ, a bacterial homolog of tubulin, is well established as forming the cytoskeletal framework for the cytokinetic ring. Recent work has shown that purified FtsZ, in the absence of any other division proteins, can assemble ...
    • FtsZ Protofilament Curvature Is the Opposite of Tubulin Rings. 

      Housman, Max; Milam, Sara L; Moore, Desmond A; Osawa, Masaki; Erickson, Harold P (Biochemistry, 2016-07)
      FtsZ protofilaments (pfs) form the bacterial cytokinetic Z ring. Previous work suggested that a conformational change from straight to curved pfs generated the constriction force. In the simplest model, the C-terminal membrane ...
    • Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division. 

      Kiro, Ruth; Molshanski-Mor, Shahar; Yosef, Ido; Milam, Sara L; Erickson, Harold P; Qimron, Udi (Proceedings of the National Academy of Sciences of the United States of America, 2013-11-11)
      Bacteriophages take over host resources primarily via the activity of proteins expressed early in infection. One of these proteins, produced by the Escherichia coli phage T7, is gene product (Gp) 0.4. Here, we show that ...
    • High-resolution crystal structures of Escherichia coli FtsZ bound to GDP and GTP. 

      Schumacher, Maria A; Ohashi, Tomoo; Corbin, Lauren; Erickson, Harold P (Acta crystallographica. Section F, Structural biology communications, 2020-02-05)
      Bacterial cytokinesis is mediated by the Z-ring, which is formed by the prokaryotic tubulin homolog FtsZ. Recent data indicate that the Z-ring is composed of small patches of FtsZ protofilaments that travel around the bacterial ...
    • How bacterial cell division might cheat turgor pressure - a unified mechanism of septal division in Gram-positive and Gram-negative bacteria. 

      Erickson, Harold P (BioEssays : news and reviews in molecular, cellular and developmental biology, 2017-08)
      An important question for bacterial cell division is how the invaginating septum can overcome the turgor force generated by the high osmolarity of the cytoplasm. I suggest that it may not need to. Several studies in Gram-negative ...
    • How Teichoic Acids Could Support a Periplasm in Gram-Positive Bacteria, and Let Cell Division Cheat Turgor Pressure. 

      Erickson, Harold P (Frontiers in microbiology, 2021-01)
      The cytoplasm of bacteria is maintained at a higher osmolality than the growth medium, which generates a turgor pressure. The cell membrane (CM) cannot support a large turgor, so there are two possibilities for transferring ...
    • How the kinetochore couples microtubule force and centromere stretch to move chromosomes. 

      Suzuki, Aussie; Badger, Benjamin L; Haase, Julian; Ohashi, Tomoo; Erickson, Harold P; Salmon, Edward D; Bloom, Kerry (Nature cell biology, 2016-04)
      The Ndc80 complex (Ndc80, Nuf2, Spc24 and Spc25) is a highly conserved kinetochore protein essential for end-on anchorage to spindle microtubule plus ends and for force generation coupled to plus-end polymerization ...
    • Inside-out Z rings--constriction with and without GTP hydrolysis. 

      Osawa, Masaki; Erickson, Harold P (Molecular microbiology, 2011-07)
      The bacterial tubulin homologue FtsZ forms a ring-like structure called the Z ring that drives cytokinesis. We showed previously that FtsZ-YFP-mts, which has a short amphipathic helix (mts) on its C terminus that inserts ...
    • Irisin and FNDC5 in retrospect: An exercise hormone or a transmembrane receptor? 

      Erickson, Harold P (Adipocyte, 2013-10-01)
      FNDC5 (fibronectin domain-containing [protein] 5) was initially discovered and characterized by two groups in 2002. In 2011 FNDC5 burst into prominence as the parent of irisin, a small protein containing the fibronectin ...
    • L form bacteria growth in low-osmolality medium. 

      Osawa, Masaki; Erickson, Harold P (Microbiology (Reading, England), 2019-08)
      L form bacteria do not have a cell wall and are thought to require medium of high osmolality for survival and growth. In this study we tested whether L forms can adapt to growth in lower osmolality medium. We first tested ...
    • Liposome division by a simple bacterial division machinery. 

      Osawa, Masaki; Erickson, Harold P (Proceedings of the National Academy of Sciences of the United States of America, 2013-07)
      We previously reconstituted Z rings in tubular multilamellar liposomes with FtsZ-YFP-mts, where mts is a membrane-targeting amphiphilic helix. These reconstituted Z rings generated a constriction force but did not divide ...
    • Microtubule Assembly from Single Flared Protofilaments-Forget the Cozy Corner? 

      Erickson, Harold P (Biophysical journal, 2019-06)
      A paradigm shift for models of MT assembly is suggested by a recent cryo-electron microscopy study of microtubules (MTs). Previous assembly models have been based on the two-dimensional lattice of the MT wall, where incoming ...