Browsing by Author "Kornbluth, Sally"
Now showing items 1-13 of 13
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A network of substrates of the E3 ubiquitin ligases MDM2 and HUWE1 control apoptosis independently of p53.
(Sci Signal, 2013-05-07)In the intrinsic pathway of apoptosis, cell-damaging signals promote the release of cytochrome c from mitochondria, triggering activation of the Apaf-1 and caspase-9 apoptosome. The ubiquitin E3 ligase MDM2 decreases the ... -
Across the meiotic divide - CSF activity in the post-Emi2/XErp1 era.
(J Cell Sci, 2008-11-01)Vertebrate eggs are arrested at the metaphase stage of meiosis II. Only upon fertilization will the metaphase-II-arrested eggs exit meiosis II and enter interphase. In 1971, Masui and Markert injected egg extracts into a ... -
Apoptosis in Drosophila: neither fish nor fowl (nor man, nor worm).
(J Cell Sci, 2005-05-01)Studies in a wide variety of organisms have produced a general model for the induction of apoptosis in which multiple signaling pathways lead ultimately to activation of the caspase family of proteases. Once activated, these ... -
Control of Emi2 activity and stability through Mos-mediated recruitment of PP2A.
(Proc Natl Acad Sci U S A, 2007-10-16)Before fertilization, vertebrate eggs are arrested in meiosis II by cytostatic factor (CSF), which holds the anaphase-promoting complex (APC) in an inactive state. It was recently reported that Mos, an integral component ... -
Differential Apaf-1 levels allow cytochrome c to induce apoptosis in brain tumors but not in normal neural tissues.
(Proc Natl Acad Sci U S A, 2007-12-26)Brain tumors are typically resistant to conventional chemotherapeutics, most of which initiate apoptosis upstream of mitochondrial cytochrome c release. In this study, we demonstrate that directly activating apoptosis downstream ... -
Emi2-mediated inhibition of E2-substrate ubiquitin transfer by the anaphase-promoting complex/cyclosome through a D-box-independent mechanism.
(Mol Biol Cell, 2010-08-01)Vertebrate eggs are arrested at Metaphase II by Emi2, the meiotic anaphase-promoting complex/cyclosome (APC/C) inhibitor. Although the importance of Emi2 during oocyte maturation has been widely recognized and its regulation ... -
Engineering a BCR-ABL-activated caspase for the selective elimination of leukemic cells.
(Proc Natl Acad Sci U S A, 2013-02-05)Increased understanding of the precise molecular mechanisms involved in cell survival and cell death signaling pathways offers the promise of harnessing these molecules to eliminate cancer cells without damaging normal cells. ... -
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
(Genes Dev, 2007-04-01)In response to DNA damage, p53 undergoes post-translational modifications (including acetylation) that are critical for its transcriptional activity. However, the mechanism by which p53 acetylation is regulated is still ... -
Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase.
(J Cell Biol, 2005-04-11)Degradation of specific protein substrates by the anaphase-promoting complex/cyclosome (APC) is critical for mitotic exit. We have identified the protein Xenopus nuclear factor 7 (Xnf7) as a novel APC inhibitor able to regulate ... -
Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication.
(Genes Dev, 2007-09-15)The MCM2-7 helicase complex is loaded on DNA replication origins during the G1 phase of the cell cycle to license the origins for replication in S phase. How the initiator primase-polymerase complex, DNA polymerase alpha ... -
Suppression of DNA-damage checkpoint signaling by Rsk-mediated phosphorylation of Mre11.
(Proc Natl Acad Sci U S A, 2013-12-17)Ataxia telangiectasia mutant (ATM) is an S/T-Q-directed kinase that is critical for the cellular response to double-stranded breaks (DSBs) in DNA. Following DNA damage, ATM is activated and recruited by the MRN protein complex ... -
The Trim39 ubiquitin ligase inhibits APC/CCdh1-mediated degradation of the Bax activator MOAP-1.
(J Cell Biol, 2012-04-30)Proapoptotic Bcl-2 family members, such as Bax, promote release of cytochrome c from mitochondria, leading to caspase activation and cell death. It was previously reported that modulator of apoptosis protein 1 (MOAP-1), ... -
Ubiquitylation of p53 by the APC/C inhibitor Trim39.
(Proc Natl Acad Sci U S A, 2012-12-18)Tripartite motif 39 (Trim39) is a RING domain-containing E3 ubiquitin ligase able to inhibit the anaphase-promoting complex (APC/C) directly. Through analysis of Trim39 function in p53-positive and p53-negative cells, we ...
