Browsing by Author "Kwun, Jean"
Now showing items 1-12 of 12
-
A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines.
(Communications biology, 2021-11)Multiplex immunoassays with acellular antigens are well-established based on solid-phase platforms such as the Luminex® technology. Cell barcoding by amine-reactive fluorescent dyes enables analogous cell-based multiplex ... -
Allo-Specific Humoral Responses: New Methods for Screening Donor-Specific Antibody and Characterization of HLA-Specific Memory B Cells.
(Frontiers in immunology, 2021-01)Antibody-mediated allograft rejection (AMR) causes more kidney transplant failure than any other single cause. AMR is mediated by antibodies recognizing antigens expressed by the graft, and antibodies generated against major ... -
Blocking CCL8-CCR8-Mediated Early Allograft Inflammation Improves Kidney Transplant Function.
(Journal of the American Society of Nephrology : JASN, 2022-08-16)<h4>Background</h4>In kidney transplantation, early allograft inflammation impairs long-term allograft function. However, precise mediators of early kidney allograft inflammation are unclear, making it challenging to design ... -
Cultured thymus tissue implementation promotes donor-specific tolerance to allogeneic heart transplants.
(JCI insight, 2020-04-30)Eighty-six infants born without a thymus have been treated with allogeneic cultured thymus tissue implantation (CTTI). These infants, who lack T cells and are profoundly immunodeficient at birth, after CTTI from an unmatched ... -
Donor apoptotic cell-based therapy for effective inhibition of donor-specific memory T and B cells to promote long-term allograft survival in allosensitized recipients.
(American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 2020-10)Allosensitization constitutes a major barrier in transplantation. Preexisting donor-reactive memory T and B cells and preformed donor-specific antibodies (DSAs) have all been implicated in accelerated allograft rejection ... -
Impact of Leukocyte Function-Associated Antigen-1 Blockade on Endogenous Allospecific T Cells to Multiple Minor Histocompatibility Antigen Mismatched Cardiac Allograft.
(Transplantation, 2015-12)BACKGROUND: Blocking leukocyte function-associated antigen (LFA)-1 in organ transplant recipients prolongs allograft survival. However, the precise mechanisms underlying the therapeutic potential of LFA-1 blockade in preventing ... -
Interleukin-15 receptor blockade in non-human primate kidney transplantation.
(Transplantation, 2010-04-27)BACKGROUND: Interleukin (IL)-15 is a chemotactic factor to T cells. It induces proliferation and promotes survival of activated T cells. IL-15 receptor blockade in mouse cardiac and islet allotransplant models has led to ... -
Prevention trumps treatment of antibody-mediated transplant rejection.
(J Clin Invest, 2010-04)Belying the spectacular success of solid organ transplantation and improvements in immunosuppressive therapy is the reality that long-term graft survival rates remain relatively unchanged, in large part due to chronic and ... -
Primary vascularization of the graft determines the immunodominance of murine minor H antigens during organ transplantation.
(J Immunol, 2011-10-15)Grafts can be rejected even when matched for MHC because of differences in the minor histocompatibility Ags (mH-Ags). H4- and H60-derived epitopes are known as immunodominant mH-Ags in H2(b)-compatible BALB.B to C57BL/6 ... -
Single cell transcriptomics of mouse kidney transplants reveals a myeloid cell pathway for transplant rejection.
(JCI insight, 2020-10)Myeloid cells are increasingly recognized as major players in transplant rejection. Here, we used a murine kidney transplantation model and single cell transcriptomics to dissect the contribution of myeloid cell subsets ... -
The role of B cells in solid organ transplantation.
(Semin Immunol, 2012-04)The role of antibodies in chronic injury to organ transplants has been suggested for many years, but recently emphasized by new data. We have observed that when immunosuppressive potency decreases either by intentional weaning ...
