Browsing by Author "Rountree, Wes"
Now showing items 1-15 of 15
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Association of HIV-1 Envelope-Specific Breast Milk IgA Responses with Reduced Risk of Postnatal Mother-to-Child Transmission of HIV-1.
(J Virol, 2015-10)UNLABELLED: Infants born to HIV-1-infected mothers in resource-limited areas where replacement feeding is unsafe and impractical are repeatedly exposed to HIV-1 throughout breastfeeding. Despite this, the majority of infants ... -
Comparison of Detection Limits of Fourth- and Fifth-Generation Combination HIV Antigen-Antibody, p24 Antigen, and Viral Load Assays on Diverse HIV Isolates.
(Journal of clinical microbiology, 2018-08)Detection of acute HIV infection is critical for HIV public health and diagnostics. Clinical fourth-generation antigen (Ag)/antibody (Ab) combination (combo) and p24 Ag immunoassays have enhanced detection of acute infection ... -
Computational analysis of antibody dynamics identifies recent HIV-1 infection.
(JCI insight, 2017-12-21)Accurate HIV-1 incidence estimation is critical to the success of HIV-1 prevention strategies. Current assays are limited by high false recent rates (FRRs) in certain populations and a short mean duration of recent infection ... -
Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays
(Journal of Immunological Methods, 2014-01-01)Luminex bead array assays are widely used for rapid biomarker quantification due to the ability to measure up to 100 unique analytes in a single well of a 96-well plate. There has been, however, no comprehensive analysis ... -
Development of an international external quality assurance program for HIV-1 incidence using the Limiting Antigen Avidity assay.
(PloS one, 2019-01)Laboratory assays for identifying recent HIV-1 infections are widely used for estimating incidence in cross-sectional population-level surveys in global HIV-1surveillance. Adequate assay and laboratory performance are required ... -
Establishment and maintenance of a PBMC repository for functional cellular studies in support of clinical vaccine trials
(Journal of Immunological Methods, 2014-01-01)A large repository of cryopreserved peripheral blood mononuclear cells (PBMCs) samples was created to provide laboratories testing the specimens from human immunodeficiency virus-1 (HIV-1) vaccine clinical trials the material ... -
HIV-1 subtype C is significantly more infectious than other subtypes
(JOURNAL OF THE INTERNATIONAL AIDS SOCIETY, 2015-07) -
Laboratory accuracy improvement in the uk neqas leucocyte immunophenotyping immune monitoring program: An eleven-year review via longitudinal mixed effects modeling.
(Cytometry B Clin Cytom, 2017-05-08)BACKGROUND: The United Kingdom National External Quality Assessment Service (UK NEQAS) for Leucocyte Immunophenotyping Immune Monitoring Programme, provides external quality assessment (EQA) to non-U.S. laboratories affiliated ... -
Neonatal Rhesus Macaques Have Distinct Immune Cell Transcriptional Profiles following HIV Envelope Immunization.
(Cell reports, 2020-02)HIV-1-infected infants develop broadly neutralizing antibodies (bnAbs) more rapidly than adults, suggesting differences in the neonatal versus adult responses to the HIV-1 envelope (Env). Here, trimeric forms of HIV-1 Env ... -
Optimization and validation of a neutralizing antibody assay for HIV-1 in A3R5 cells.
(Journal of immunological methods, 2014-07)A3R5 is a human CD4(+) lymphoblastoid cell line that was engineered to express CCR5 and is useful for the detection of weak neutralizing antibody responses against tier 2 strains of HIV-1. Here we describe the optimization ... -
Statistical methods for the assessment of EQAPOL proficiency testing: ELISpot, Luminex, and Flow Cytometry.
(J Immunol Methods, 2014-07)In September 2011 Duke University was awarded a contract to develop the National Institutes of Health/National Institute of Allergy and Infectious Diseases (NIH/NIAID) External Quality Assurance Program Oversight Laboratory ... -
The Center for HIV/AIDS Vaccine Immunology (CHAVI) multi-site quality assurance program for cryopreserved human peripheral blood mononuclear cells.
(J Immunol Methods, 2014-07)The Center for HIV/AIDS Vaccine Immunology (CHAVI) consortium was established to determine the host and virus factors associated with HIV transmission, infection and containment of virus replication, with the goal of advancing ... -
The External Quality Assurance Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay
(Journal of Immunological Methods, 2014-01-01)The interferon-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay has been developed and used as an end-point assay in clinical trials for infectious diseases and cancer to detect the magnitude of antigen-specific immune ... -
Toward development of a comprehensive external quality assurance program for polyfunctional intracellular cytokine staining assays
(Journal of Immunological Methods, 2014-01-01)The External Quality Assurance Program Oversight Laboratory (EQAPOL) Flow Cytometry Program assesses the proficiency of NIH/NIAID/DAIDS-supported and potentially other interested research laboratories in performing Intracellular ... -
Variability of the IFN-γ ELISpot assay in the context of proficiency testing and bridging studies.
(J Immunol Methods, 2016-06)Assays that assess cellular mediated immune responses performed under Good Clinical Laboratory Practice (GCLP) guidelines are required to provide specific and reproducible results. Defined validation procedures are required ...
