Browsing by Author "Weinhold, Kent J"
Now showing items 1-14 of 14
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A randomized phase 2 trial of pembrolizumab versus pembrolizumab and acalabrutinib in patients with platinum-resistant metastatic urothelial cancer.
(Cancer, 2020-08-05)BACKGROUND:Inhibition of the programmed cell death protein 1 (PD-1) pathway has demonstrated clinical benefit in metastatic urothelial cancer (mUC); however, response rates of 15% to 26% highlight the need for more effective ... -
B10 Cell Frequencies and Suppressive Capacity in Myasthenia Gravis Are Associated with Disease Severity.
(Front Neurol, 2017)Myasthenia gravis (MG) is a T cell-dependent, B cell-mediated disease. The mechanisms for loss of self-tolerance in this disease are not well understood, and recently described regulatory B cell (Breg) subsets have not been ... -
Characterization of B cells in muscle-specific kinase antibody myasthenia gravis.
(Neurol Neuroimmunol Neuroinflamm, 2015-04)OBJECTIVE: To characterize B-cell subsets in patients with muscle-specific tyrosine kinase (MuSK) myasthenia gravis (MG). METHODS: In accordance with Human Immunology Project Consortium guidelines, we performed polychromatic ... -
Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling.
(PloS one, 2019-01)Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte ... -
Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study.
(Anesthesia and analgesia, 2019-05-03)Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before ... -
FlowKit: A Python Toolkit for Integrated Manual and Automated Cytometry Analysis Workflows.
(Frontiers in immunology, 2021-01)An important challenge for primary or secondary analysis of cytometry data is how to facilitate productive collaboration between domain and quantitative experts. Domain experts in cytometry laboratories and core facilities ... -
HLA class II-Restricted CD8+ T cells in HIV-1 Virus Controllers.
(Scientific reports, 2019-07-15)A paradigm shifting study demonstrated that induction of MHC class E and II-restricted CD8+ T cells was associated with the clearance of SIV infection in rhesus macaques. Another recent study highlighted the presence of ... -
Immunization with cocktail of HIV-derived peptides in montanide ISA-51 is immunogenic, but causes sterile abscesses and unacceptable reactogenicity.
(PLoS One, 2010-08-10)BACKGROUND: A peptide vaccine was produced containing B and T cell epitopes from the V3 and C4 Envelope domains of 4 subtype B HIV-1 isolates (MN, RF, CanO, & Ev91). The peptide mixture was formulated as an emulsion in incomplete ... -
Normative dataset for plasma cytokines in healthy human adults.
(Data in brief, 2021-04)We determined normative data for plasma cytokines established from a cohort of 126 carefully screened healthy adults aged 18 to 64 years. Participants were enrolled to ensure an even age and sex distribution and to include ... -
Prolonged B-cell depletion in MuSK myasthenia gravis following rituximab treatment.
(Muscle Nerve, 2013-12) -
Setting objective thresholds for rare event detection in flow cytometry.
(J Immunol Methods, 2014-07)The accurate identification of rare antigen-specific cytokine positive cells from peripheral blood mononuclear cells (PBMC) after antigenic stimulation in an intracellular staining (ICS) flow cytometry assay is challenging, ... -
The INTUIT Study: Investigating Neuroinflammation Underlying Postoperative Cognitive Dysfunction.
(Journal of the American Geriatrics Society, 2019-01-23)BACKGROUND/OBJECTIVES:Every year, up to 40% of the more than 16 million older Americans who undergo anesthesia/surgery develop postoperative cognitive dysfunction (POCD) or delirium. Each of these distinct syndromes is associated ... -
Toward development of a comprehensive external quality assurance program for polyfunctional intracellular cytokine staining assays
(Journal of Immunological Methods, 2014-01-01)The External Quality Assurance Program Oversight Laboratory (EQAPOL) Flow Cytometry Program assesses the proficiency of NIH/NIAID/DAIDS-supported and potentially other interested research laboratories in performing Intracellular ... -
Transcriptional Profiling of CD8+ CMV-Specific T Cell Functional Subsets Obtained Using a Modified Method for Isolating High-Quality RNA From Fixed and Permeabilized Cells.
(Frontiers in immunology, 2020-01)Previous studies suggest that the presence of antigen-specific polyfunctional T cells is correlated with improved pathogen clearance, disease control, and clinical outcomes; however, the molecular mechanisms responsible ...
