Browsing by Department "Biochemistry"
Now showing items 1-20 of 153
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A Comprehensive Study of Guanosine-5'-triphosphate Hydrolysis by the Bacterial Cell Division Protein FtsZ
(2018)The bacterial protein FtsZ plays a vital role in cytokinesis in prokaryotes as it polymerizes to form an FtsZ ring (Z ring) at the division septum midcell. FtsZ exhibits a GTP hydrolysis activity and attempts have been made ... -
A Genetic Screen for the Identification of Mutants Hypersensitive to 5-Azacytidine
(2010)A DNA-protein crosslink is a covalent bond between DNA and a protein. It is a type of DNA damage that is relatively understudied. This study reports on the identification of a set of transposon mutants sensitive to 5-azacytidine, ... -
A Novel DNA Damage Response Network Associated with the CTD of RNA Polymerase II
(2012)Since RNA Polymerase II (RNAPII) transcribes much of the genome, it is well situated to encounter and initiate a response to various types of DNA damage. However, to date very little is known about any response of RNAPII ... -
A tale of two metallophosphatases: biochemical and functional characterization of novel substrates of PP1 and MESH1
(2017)Addition and removal of phosphate is an important post-translational modification involved in cellular signaling. The enzymes responsible for removing this phosphorylation mark, called phosphatases, play a vital role in ... -
Analysis and Redesign of Protein-Protein Interactions: A Hotspot-Centric View
(2010)<p>One of the most significant discoveries from mutational analysis of protein interfaces is that often a large percentage of interface residues negligibly perturb the binding energy upon mutation, while residues in a few ... -
Aqueous Desolvation and Molecular Recognition: Experimental and Computational Studies of a Novel Host-Guest System Based on Cucurbit[7]uril
(2012)Molecular recognition is arguably the most elementary physical process essential for life that arises at the molecular scale. Molecular recognition drives events across virtually all length scales, from the folding of proteins ... -
Biased Signaling at the β2-adrenergic Receptor is established by Receptor-Transducer Interactions
(2018)β-Adrenergic receptors (βAR) are one of the key modulators of cardio-pulmonary functions and belong to a large family of membrane proteins, termed as G-protein coupled receptors (GPCRs). β-blockers (βAR antagonists) and ... -
Biochemical and Genetic Studies of UDP-2,3-Diacylglucosamine Hydrolysis in Lipid A Biosynthesis
(2014)The outer-leaflet of the outer membrane of Gram-negative bacteria is composed of lipopolysaccharide (LPS), which is attached to the membrane via a hexa-acylated saccharolipid called lipid A. The fourth step of ... -
Biochemical and structural mechanisms of multidrug efflux pump transcription regulators, Neisseria gonorrhoeae MtrR and Escherichia coli MprA
(2021)As bacterial resistance to multiple antibiotics continues to become a growing problem across the globe, the imperativeness for understanding mechanisms of antibiotic and multidrug resistance is increasingly apparent. Currently, ... -
Biochemical and Structural Studies on PrfA, the Transcriptional Regulator of Virulence in Listeria monocytogenes
(2016)AbstractListeria monocytogenes is a gram-positive soil saprophytic bacterium that is capable of causing fatal infection in humans. The main virulence regulator PrfA, a member of the Crp/FNR family of transcriptional regulators, ... -
Biochemical Characterization of Human Exonuclease 1 Protein-protein Interactions
(2016)Human Exonuclease 1 (Exo1) plays important roles in numerous DNA metabolic/repair pathways including DNA mismatch repair, DNA double strand break repair, Okazaki fragment maturation etc. The nuclease activity of Exo1 is ... -
Biochemical Characterization of Lipid A Modification Enzymes From Rhizobium leguminosarum and Rhizobium etli
(2010)The lipid A component of lipopolysaccharide (LPS) in the nitrogen-fixing plant endosymbionts <italic>Rhizobium leguminosarum</italic> and <italic>Rhizobium etli</italic> is strikingly different when compared to that of enteric ... -
Building Better Backbones: Visualizations, Analyses, and Tools for Higher Quality Macromolecular Structure Models
(2010)In this work, I develop computational and visual tools for analyzing and manipulating the backbone of macromolecules, and I demonstrate that these tools support building better structures than currently done. These visualization ... -
Calcium/Calmodulin-Dependent Protein Kinase II Serves as a Biochemical Integrator of Calcium Signals for the Induction of Synaptic Plasticity
(2016)Repetitive Ca2+ transients in dendritic spines induce various forms of synaptic plasticity by transmitting information encoded in their frequency and amplitude. CaMKII plays a critical role in decoding these Ca2+ signals ... -
Characterization of a Full-Length TTP Family Member Association with RNA Sequence Elements
(2016)Post-transcriptional regulation of cytoplasmic mRNAs is an efficient mechanism of regulating the amounts of active protein within a eukaryotic cell. RNA sequence elements located in the untranslated regions of mRNAs can ... -
Characterization of a TrkB-derived Phosphopeptide Inhibitor of PLCγ1
(2018)Epilepsy is a syndrome that affects about 65 million people around the world. About 150,000 new cases of epilepsy are reported in the United States every year. Temporal lobe epilepsy (TLE) is the most common form of human ... -
Characterization of Beta-arrestin-Modulated Lipid Kinase Activities for Diacylglycerol and Phosphatidylinositol 4-Phosphate
(2007-05-10)The study of arrestins as regulators of seven transmembrane receptor (7TMR) signaling has revealed multiple levels of complexity, initiating desensitization of G protein activity and coordination of receptor internalization ... -
Characterization of dCDK12, hCDK12, and hCDK13 in the Context of RNA Polymerase II CTD Phosphorylation and Transcription-Associated Events
(2014)Eukaryotic RNA polymerase II (RNAPII) not only synthesizes mRNA, but also coordinates transcription-related processes through the post-translational modification of its unique C-terminal repeat domain (CTD). The CTD is an ... -
Characterization of LpxC inhibitors and resistant mutants
(2012)LpxC, the deacetylase that catalyzes the second and committed step of lipid A biosynthesis in <italic>E. coli</italic>, is an essential enzyme for virtually all Gram-negative bacteria and one of the most promising novel ... -
Characterization of Peripheral-Membrane Enzymes Required for Lipid A Biosynthesis in Gram-Negative Bacteria
(2010)Gram-negative bacteria possess an asymmetric outer membrane in which the inner leaflet is composed primarily of phospholipids while the outer leaflet contains both phospholipids and lipopolysaccharide (LPS). LPS forms a ...
