ALERT: This system is being upgraded on Tuesday December 12. It will not be available
for use for several hours that day while the upgrade is in progress. Deposits to DukeSpace
will be disabled on Monday December 11, so no new items are to be added to the repository
while the upgrade is in progress. Everything should be back to normal by the end of
day, December 12.
Browsing by Subject "Antibodies"
Now showing items 1-20 of 24
-
A beta-adrenergic receptor kinase-like enzyme is involved in olfactory signal termination.
(Proc Natl Acad Sci U S A, 1993-02-15)We have previously shown that second-messenger-dependent kinases (cAMP-dependent kinase, protein kinase C) in the olfactory system are essential in terminating second-messenger signaling in response to odorants. We now document ... -
A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines.
(Communications biology, 2021-11)Multiplex immunoassays with acellular antigens are well-established based on solid-phase platforms such as the Luminex® technology. Cell barcoding by amine-reactive fluorescent dyes enables analogous cell-based multiplex ... -
ACE-inhibition increases podocyte number in experimental glomerular disease independent of proliferation.
(Journal of the renin-angiotensin-aldosterone system : JRAAS, 2015-06)<h4>Objective</h4>The objective of this article is to test the effects of angiotensin-converting enzyme (ACE)-inhibition on glomerular epithelial cell number in an inducible experimental model of focal segmental ... -
Antibody-mediated Immunotherapy of Brain Tumors
(2017)Conventional therapy for malignant glioma (MG) fails to specifically target tumor cells. In contrast, immunotherapy offers an exquisitely precise approach, and substantial evidence indicates that if appropriately redirected, ... -
Costimulation blockade alters germinal center responses and prevents antibody-mediated rejection.
(Am J Transplant, 2014-01)De novo donor-specific antibody (DSA) after organ transplantation promotes antibody-mediated rejection (AMR) and causes late graft loss. Previously, we demonstrated that depletion using anti-CD3 immunotoxin combined with ... -
Defining the Role of Antibodies in Protection Against Cytomegalovirus Acquisition and Congenital Disease for Rational Vaccine Design
(2018)Human cytomegalovirus (HCMV) is the most common cause of congenital infection worldwide, impacting 1 in 150 live-born infants. Children afflicted by congenital HCMV frequently suffer from lifelong, debilitating neurologic ... -
Design of protease-resistant peptide ligands for the purification of antibodies from human plasma.
(Journal of chromatography. A, 2016-05)A strategy is presented for developing variants of peptide ligands with enhanced biochemical stability for the purification of antibodies from animal sera. Antibody-binding sequences HWRGWV, HYFKFD, and HFRRHL, previously ... -
Emphysema-associated Autoreactive Antibodies Exacerbate Post-Lung Transplant Ischemia-Reperfusion Injury.
(American journal of respiratory cell and molecular biology, 2019-06)Chronic obstructive pulmonary disease-associated chronic inflammation has been shown to lead to an autoimmune phenotype characterized in part by the presence of lung autoreactive antibodies. We hypothesized that ... -
HPV16 antibodies as risk factors for oropharyngeal cancer and their association with tumor HPV and smoking status.
(Oral Oncol, 2015-07)BACKGROUND: Antibodies (Abs) to the HPV16 proteome increase risk for HPV-associated OPC (HPVOPC). The goal of this study was to investigate the association of a panel of HPV16 Abs with risk for OPC as well as the association ... -
In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. I. The architecture and dynamics of responding cell populations.
(J Exp Med, 1991-05-01)After primary immunization with an immunogenic conjugate of (4-hydroxy-3-nitrophenyl)acetyl, two anatomically and phenotypically distinct populations of antibody-forming cells arise in the spleen. As early as 2 ... -
In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. V. Affinity maturation develops in two stages of clonal selection.
(J Exp Med, 1998-03-16)To examine the role of germinal centers (GCs) in the generation and selection of high affinity antibody-forming cells (AFCs), we have analyzed the average affinity of (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific AFCs and ... -
Indications for and outcomes of therapeutic plasma exchange after cardiac transplantation: A single center retrospective study.
(Journal of clinical apheresis, 2018-08)INTRODUCTION:Limited data are available describing indications for and outcomes of therapeutic plasma exchange (TPE) in cardiac transplantation. METHODS:In a retrospective study of patients who underwent cardiac transplantation ... -
Induction of anti-myelin antibodies in EAE and their possible role in demyelination.
(J Neurosci Res, 1991-12)Experimental allergic encephalomyelitis is characterized by invasion of lymphocytes and macrophages into the central nervous system resulting in inflammation, edema, and demyelination. Sera from Lewis rats from 7-95 days ... -
Interrogation of individual intratumoral B lymphocytes from lung cancer patients for molecular target discovery.
(Cancer Immunol Immunother, 2016-02)Intratumoral B lymphocytes are an integral part of the lung tumor microenvironment. Interrogation of the antibodies they express may improve our understanding of the host response to cancer and could be useful in elucidating ... -
Neutralization Takes Precedence Over IgG or IgA Isotype-related Functions in Mucosal HIV-1 Antibody-mediated Protection.
HIV-1 infection occurs primarily through mucosal transmission. Application of biologically relevant mucosal models can advance understanding of the functional properties of antibodies that mediate HIV protection, thereby ... -
Polyethylene Glycol-conjugated L-asparaginase versus native L-asparaginase in combination with standard agents for children with acute lymphoblastic leukemia in second bone marrow relapse: a Children's Oncology Group Study (POG 8866).
(Journal of pediatric hematology/oncology, 2011-12)<h4>Background</h4>Administration of L-asparaginase is limited by hypersensitivity reactions mediated by anti-asparaginase antibodies. To overcome this problem, native Escherichia coli L-asparaginase was conjugated ... -
Pretargeting and Bioorthogonal Click Chemistry-Mediated Endogenous Stem Cell Homing for Heart Repair.
(ACS nano, 2018-12)Stem cell therapy is one of the promising strategies for the treatment of ischemic heart disease. However, the clinical application of stem cells transplantation is limited by low cell engraftment in the infarcted myocardium. ... -
Rational Vaccine Design Against Cytomegalovirus
(2022)Human cytomegalovirus (CMV) is the most common cause of congenital infection worldwide, affecting approximately 1 in 150 infants, and is a leading cause of morbidity and mortality among transplant recipients. Congenital ... -
Relaxed negative selection in germinal centers and impaired affinity maturation in bcl-xL transgenic mice.
(J Exp Med, 1999-08-02)The role of apoptosis in affinity maturation was investigated by determining the affinity of (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific antibody-forming cells (AFCs) and serum antibody in transgenic mice that overexpress ... -
The Impact of Antibody Biophysical Properties on Antigen Recognition and Fc Effector Functions
(2021)Vaccines save millions of lives every year primarily through the induction of antigen-specific antibodies; however, there is a critical lack of understanding of the mechanistic underpinings of vaccine-elicited, antibody-mediated ...
