Browsing by Subject "Bacterial Proteins"
Now showing items 1-20 of 40
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A DNA mimic: the structure and mechanism of action for the anti-repressor protein AbbA.
(Journal of molecular biology, 2014-05)Bacteria respond to adverse environmental conditions by switching on the expression of large numbers of genes that enable them to adapt to unfavorable circumstances. In Bacillus subtilis, many adaptive genes are under the ... -
Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro.
(J Cell Biol, 1996-11)In S. cerevisiae lacking SHR3, amino acid permeases specifically accumulate in membranes of the endoplasmic reticulum (ER) and fail to be transported to the plasma membrane. We examined the requirements of transport of the ... -
Bacterial effector activates jasmonate signaling by directly targeting JAZ transcriptional repressors.
(PLoS pathogens, 2013-10-31)Gram-negative bacterial pathogens deliver a variety of virulence proteins through the type III secretion system (T3SS) directly into the host cytoplasm. These type III secreted effectors (T3SEs) play an essential role in ... -
Binding of MetJ repressor to specific and nonspecific DNA and effect of S-adenosylmethionine on these interactions.
(Biochemistry, 2010-04-20)We have used analytical ultracentrifugation to characterize the binding of the methionine repressor protein, MetJ, to synthetic oligonucleotides containing zero to five specific recognition sites, called metboxes. For all ... -
Bonds between fibronectin and fibronectin-binding proteins on Staphylococcus aureus and Lactococcus lactis.
(Langmuir, 2010-07-06)Bacterial cell-wall-associated fibronectin binding proteins A and B (FnBPA and FnBPB) form bonds with host fibronectin. This binding reaction is often the initial step in prosthetic device infections. Atomic force microscopy ... -
Caulobacter crescentus Adapts to Phosphate Starvation by Synthesizing Anionic Glycoglycerolipids and a Novel Glycosphingolipid.
(mBio, 2019-04-02)Caulobacter crescentus adapts to phosphate starvation by elongating its cell body and a polar stalk structure. The stalk is an extension of the Gram-negative envelope containing inner and outer membranes as well ... -
Cell division without FtsZ--a variety of redundant mechanisms.
(Molecular microbiology, 2010-10)Until 1998 it looked like all bacteria and archaea used a universal cytokinetic machine based on FtsZ. A dozen completely sequenced bacterial genomes all had an ftsZ gene, as did the several sequenced archaeal genomes. Then ... -
Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings.
(Methods in enzymology, 2009-01)We have developed a system for producing tubular multilamellar liposomes that incorporate the protein FtsZ on the inside. We start with a mixture of spherical multilamellar liposomes with FtsZ initially on the outside. Shearing ... -
Chemical shift assignments and secondary structure prediction of the phosphorelay protein VanU from Vibrio anguillarum.
(Biomolecular NMR assignments, 2014-04)Vibrio anguillarum is a biofilm forming Gram-negative bacterium that survives prolonged periods in seawater and causes vibriosis in marine life. A quorum-sensing signal transduction pathway initiates biofilm formation in ... -
Conformational changes of FtsZ reported by tryptophan mutants.
(Biochemistry, 2011-05-03)E. coli FtsZ has no native tryptophan. We showed previously that the mutant FtsZ L68W gave a 2.5-fold increase in trp fluorescence when assembly was induced by GTP. L68 is probably buried in the protofilament interface upon ... -
Conformational kinetics reveals affinities of protein conformational states.
(Proc Natl Acad Sci U S A, 2015-07-28)Most biological reactions rely on interplay between binding and changes in both macromolecular structure and dynamics. Practical understanding of this interplay requires detection of critical intermediates and determination ... -
Curved FtsZ protofilaments generate bending forces on liposome membranes.
(The EMBO journal, 2009-11)We have created FtsZ-YFP-mts where an amphipathic helix on the C-terminus tethers FtsZ to the membrane. When incorporated inside multi-lamellar tubular liposomes, FtsZ-YFP-mts can assemble Z rings that generate a constriction ... -
Differential Translocation of Host Cellular Materials into the Chlamydia trachomatis Inclusion Lumen during Chemical Fixation.
(PLoS One, 2015)Chlamydia trachomatis manipulates host cellular pathways to ensure its proliferation and survival. Translocation of host materials into the pathogenic vacuole (termed 'inclusion') may facilitate nutrient acquisition and ... -
Dual impact of elevated temperature on plant defence and bacterial virulence in Arabidopsis.
(Nature communications, 2017-11-27)Environmental conditions profoundly affect plant disease development; however, the underlying molecular bases are not well understood. Here we show that elevated temperature significantly increases the susceptibility of ... -
Elucidating the impact of microbial community biodiversity on pharmaceutical biotransformation during wastewater treatment.
(Microbial biotechnology, 2018-11)In addition to removing organics and other nutrients, the microorganisms in wastewater treatment plants (WWTPs) biotransform many pharmaceuticals present in wastewater. The objective of this study was to examine the relationship ... -
Environmental fate and effects of Bacillus thuringiensis (Bt) proteins from transgenic crops: a review.
(Journal of agricultural and food chemistry, 2005-06)This paper reviews the scientific literature addressing the environmental fate and nontarget effects of the Cry protein toxins from Bacillus thuringiensis (Bt), specifically resulting from their expression in transgenic ... -
FtsZ at mid-cell is essential in Escherichia coli until the late stage of constriction.
(Microbiology (Reading, England), 2022-06)There has been recent debate as to the source of constriction force during cell division. FtsZ can generate a constriction force on tubular membranes in vitro, suggesting it may generate the constriction force in vivo. However, ... -
FtsZ filament capping by MciZ, a developmental regulator of bacterial division.
(Proceedings of the National Academy of Sciences of the United States of America, 2015-04-06)Cytoskeletal structures are dynamically remodeled with the aid of regulatory proteins. FtsZ (filamentation temperature-sensitive Z) is the bacterial homolog of tubulin that polymerizes into rings localized to cell-division ... -
FtsZ in bacterial cytokinesis: cytoskeleton and force generator all in one.
(Microbiology and molecular biology reviews : MMBR, 2010-12)FtsZ, a bacterial homolog of tubulin, is well established as forming the cytoskeletal framework for the cytokinetic ring. Recent work has shown that purified FtsZ, in the absence of any other division proteins, can assemble ... -
FtsZ Protofilament Curvature Is the Opposite of Tubulin Rings.
(Biochemistry, 2016-07)FtsZ protofilaments (pfs) form the bacterial cytokinetic Z ring. Previous work suggested that a conformational change from straight to curved pfs generated the constriction force. In the simplest model, the C-terminal membrane ...
