Browsing by Subject "Bioengineering"
Now showing items 1-20 of 29
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A Toolbox for Observing and Modulating the Gut-Brain Axis
(2022)An estimated 10% of people worldwide have an enteric nervous system (ENS) related illness including irritable bowel syndrome (IBS), diabetes, colorectal cancer, fecal incontinence, and chronic constipation or diarrhea. Current ... -
Bench to bedside: A Bispecific Antibody for treating Brain Tumors
(2019)Malignant gliomas are the most common primary brain tumor in adults, with an incidence of five cases per 100,000 persons per year. Grade IV glioblastoma is the most aggressive form and prognosis remains poor despite the ... -
Bioengineered human myobundles mimic clinical responses of skeletal muscle to drugs.
(Elife, 2015-01-09)Existing in vitro models of human skeletal muscle cannot recapitulate the organization and function of native muscle, limiting their use in physiological and pharmacological studies. Here, we demonstrate engineering ... -
Bioinformatics and Molecular Approaches for the Construction of Biological Artificial Cartilage
(2018)Osteoarthritis (OA) is one of the leading causes of disability in the United States, afflicting over 27 million Americans and imposing an economic burden of more than $128 billion each year (1, 2). OA is characterized by ... -
Blast-Induced Neurotrauma and the Cavitation Mechanism of Injury
(2019)Traumatic brain injuries (TBIs) are a major public health concern and socioeconomic burden worldwide. In recent years, brain injuries in US service personnel have focused attention on TBI affecting the military population ... -
Design of Biomaterials Towards Endogenous Bone Regeneration
(2020)Bone grafting is one of the most commonly used surgical methods to augment bone regeneration in orthopedic procedure. While using natural bones, such as autograft and allograft are considered as the gold standard techniques, ... -
Developing a Fibrotic Phenotype in a 3D Human Skeletal Muscle Microphysiological System
(2022)Muscle fibrosis is caused by muscle injury, dystrophy, sarcopenia, and rheumatoid arthritis. This condition is characterized by hardening and scarring, which impairs contractile muscle function. To understand how fibrotic ... -
Developing Molecular Tools for Interrogating a Vocal Learning Avian Species
(2020)The zebra finch, an Australian songbird, is a uniquely powerful model organism for the study of vocal production learning, and its song system shares behavioral, anatomical, and genetic properties with the human spoken language ... -
Development of a Generalizable Assay for Probing the Effects of Mechanical Force on the Function of Fluorescent Proteins within Molecular Tension Sensors
(2021)The extracellular environment is a key regulator of cell behavior, providing both biochemical factors and mechanical signals to influence the form and function of cells. The process by which cells sense and respond ... -
Development of a High Performance, Biological Trickling Filter to Upgrade Raw Biogas to Renewable Natural Gas Standards
(2019)Upgrading raw biogas (~60% CH4, 40% CO2, 1000-5000 ppmv H2S) to renewable natural gas (RNG) (> 97% CH4, < 2% CO2, < 4 ppmv H2S) for injection into the grid is a desirable endeavor. RNG would allow for a clean alternative ... -
Development of a Self-Focusing Multi-Spark Shock Wave Generator for Lithotripsy
(2018)In this thesis, a self-focusing multi-spark (SFMS) shock wave generator is developed to provide flexibility in controlling the beam size and shape in an electrohydraulic shock wave lithotripter. Such a device will allow ... -
Development of Novel Antibody-Based Immunotherapies Targeting Human Chondroitin Sulfate Proteoglycan 4
(2018)Chondroitin sulfate proteoglycan 4 (CSPG4) is a promising target for cancer immunotherapy due to its high level of expression in a number of malignant tumors, and its essential role in tumor growth and progression. Clinical ... -
Development of Tunable Molecular Tension Sensors to Visualize Vinculin Loading and Detect Mechanosensitive Protein Recruitment to Focal Adhesions
(2018)Mechanical forces are potent drivers of many biological processes. The form and function of many tissues depends on cells receiving the proper mechanical signals either from neighboring cells or from the underlying matrix. ... -
Dynamic Metabolic Control Improves the Biosynthesis of Chemical Molecules in Engineered E. coli
(2022)Metabolic engineering is an effective strategy to optimize the biosynthesis of chemical molecules in genetically modified microbes. However, many current metabolic engineering strategies are limited by the requirements for ... -
Effect of Radiation on Cardiovascular Function
(2020)There is a scarcity of knowledge regarding the cardiovascular effects of low dose ionizing radiation (IR) such as the one experienced during medical tests, radiation therapy or space travel. This is becoming more of a pressing ... -
Elucidation of Context Dependent Factors Influencing CRISPR-Cas Activity
(2021)Since being developed as a tool in 2012, CRISPR-Cas9 and other CRISPR systems have revolutionized the way we manipulate biology. CRISPR systems broadly rely on a programmable guide RNA (gRNA) to enable targeted nuclease ... -
Genome Engineering Tools to Dissect Gene Regulation
(2019)Over the past several years genome and epigenome engineering has been propelled forward by CRISPR-Cas technologies. These prokaryotic defense systems work well in mammalian cells in a manner that is remarkably robust: they ... -
Genome-wide Metabolic Reconstruction and Flux Balance Analysis Modeling of Haloferax volcanii
(2018)The Archaea are an understudied domain of the tree of life, and consist of single-celled microorganisms possessing rich metabolic diversity. Archaeal metabolic capabilities are of interest for industry and basic understanding ... -
Harnessing Optical Imaging for Assessing Metabolic Reprogramming in Breast Cancer
(2020)According to the World Health Organization, there were over 2 million new breast cancer cases in 2018. This number is projected to steadily increase year after year. American Cancer Society projections for 2020 list the ... -
In Vitro Calcium Imaging of Magnetogenetic Ion Channels TRPV1FeRIC and TRPV4FeRIC
(2018)Ferritin-based magnetogenetic ion channels are promising new tools for non-invasive manipulation of ion channel activity. The use of these channels in animals has been promising but in vitro experiments in cultured cells ...
