ALERT: This system is being upgraded on Tuesday December 12. It will not be available
for use for several hours that day while the upgrade is in progress. Deposits to DukeSpace
will be disabled on Monday December 11, so no new items are to be added to the repository
while the upgrade is in progress. Everything should be back to normal by the end of
day, December 12.
Browsing by Subject "Cas9"
Now showing items 1-5 of 5
-
Cell Wall Lipids Promoting Host Angiogenesis During Mycobacterial Infection
(2018)Mycobacterial infection leads to the formation of characteristic immune cell aggregates called granulomas. In humans and animal models, tuberculous granuloma formation is accompanied by dramatic remodeling of host vasculature ... -
Creation of Versatile Cloning Platforms for Transgene Expression and Epigenome Editing and Their Application to Pancreatic Islet Biology
(2018)Insulin secreting β-cells within the pancreatic islets of Langerhans are vital to maintaining glycemic control. β-cell functional mass is lost during the progression to both Type 1 and Type 2 diabetes mellitus, resulting ... -
Elucidation of Context Dependent Factors Influencing CRISPR-Cas Activity
(2021)Since being developed as a tool in 2012, CRISPR-Cas9 and other CRISPR systems have revolutionized the way we manipulate biology. CRISPR systems broadly rely on a programmable guide RNA (gRNA) to enable targeted nuclease ... -
Examination of Endogenous Rotund Expression and Function in Developing Drosophila Olfactory System Using CRISPR-Cas9-Mediated Protein Tagging.
(G3 (Bethesda), 2015-10-23)The zinc-finger protein Rotund (Rn) plays a critical role in controlling the development of the fly olfactory system. However, little is known about its molecular function in vivo. Here, we added protein tags to the rn locus ... -
IDH1 R132H Mutations Actively Contribute to the Epigenetic State of Glioma Cells
(2019)Point mutations in the active site of isocitrate dehydrogenases 1 and 2 (\textit{IDH}) occur in the majority of WHO grade II and III gliomas, resulting in a unique milieu of signaling and metabolism. IDH1/2 active site ...
