Browsing by Subject "Cell Culture Techniques"
Now showing items 1-10 of 10
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A three-dimensional culture system recapitulates placental syncytiotrophoblast development and microbial resistance.
(Science advances, 2016-03-04)In eutherians, the placenta acts as a barrier and conduit at the maternal-fetal interface. Syncytiotrophoblasts, the multinucleated cells that cover the placental villous tree surfaces of the human placenta, are directly ... -
Diet-induced obesity alters the differentiation potential of stem cells isolated from bone marrow, adipose tissue and infrapatellar fat pad: the effects of free fatty acids.
(Int J Obes (Lond), 2013-08)INTRODUCTION: Obesity is a major risk factor for several musculoskeletal conditions that are characterized by an imbalance of tissue remodeling. Adult stem cells are closely associated with the remodeling and potential repair ... -
Efficacy and safety of ex vivo cultured adult human mesenchymal stem cells (Prochymal™) in pediatric patients with severe refractory acute graft-versus-host disease in a compassionate use study.
(Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 2011-04)Preliminary studies using directed-donor ex vivo expanded human mesenchymal stem cells (hMSCs) have shown promise in the treatment of acute graft-versus-host disease (aGVHD). However, their production is cumbersome ... -
Ex vivo expansion of murine and human hematopoietic stem cells.
(Methods Mol Biol, 2014)Hematopoietic stem cells have the capacity to self-renew and give rise to the entirety of the mature blood and immune system throughout the lifespan of an organism. Here, we describe methods to isolate and culture murine ... -
L form bacteria growth in low-osmolality medium.
(Microbiology (Reading, England), 2019-08)L form bacteria do not have a cell wall and are thought to require medium of high osmolality for survival and growth. In this study we tested whether L forms can adapt to growth in lower osmolality medium. We first tested ... -
Phosphatase and tensin homologue/protein kinase B pathway linked to motor neuron survival in human superoxide dismutase 1-related amyotrophic lateral sclerosis.
(Brain : a journal of neurology, 2011-02)Gene expression profiling has been used previously with spinal cord homogenates and laser capture microdissected motor neurons to determine the mechanisms involved in neurodegeneration in amyotrophic lateral sclerosis. However, ... -
Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung.
(Nature communications, 2015-04-13)The plasticity of differentiated cells in adult tissues undergoing repair is an area of intense research. Pulmonary alveolar type II cells produce surfactant and function as progenitors in the adult, demonstrating ... -
Rapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood.
(Stem cell reviews and reports, 2015-08)Human peripheral blood and umbilical cord blood represent attractive sources of cells for reprogramming to induced pluripotent stem cells (iPSCs). However, to date, most of the blood-derived iPSCs were generated using either ... -
Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels.
(Sci Rep, 2015-10-12)Tissue-engineered blood vessels (TEBV) can serve as vascular grafts and may also play an important role in the development of organs-on-a-chip. Most TEBV construction involves scaffolding with biomaterials such as collagen ... -
Utility of telomerase-pot1 fusion protein in vascular tissue engineering.
(Cell Transplant, 2010)While advances in regenerative medicine and vascular tissue engineering have been substantial in recent years, important stumbling blocks remain. In particular, the limited life span of differentiated cells that are harvested ...
