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Browsing by Subject "Cell Separation"
Now showing items 1-9 of 9
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A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflamed Murine Non-Lymphoid Tissues.
(PloS one, 2016-01)Flow cytometry is used extensively to examine immune cells in non-lymphoid tissues. However, a method of flow cytometric analysis that is both comprehensive and widely applicable has not been described. We developed a protocol ... -
Characterization of CD4 and CD8 T cell responses in MuSK myasthenia gravis.
(J Autoimmun, 2014-08)Muscle specific tyrosine kinase myasthenia gravis (MuSK MG) is a form of autoimmune MG that predominantly affects women and has unique clinical features, including prominent bulbar weakness, muscle atrophy, and excellent ... -
Cryptococcus gattii VGIII isolates causing infections in HIV/AIDS patients in Southern California: identification of the local environmental source as arboreal.
(PLoS Pathog, 2014-08)Ongoing Cryptococcus gattii outbreaks in the Western United States and Canada illustrate the impact of environmental reservoirs and both clonal and recombining propagation in driving emergence and expansion of microbial ... -
Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling.
(PloS one, 2019-01)Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte ... -
Ex vivo expansion of murine and human hematopoietic stem cells.
(Methods Mol Biol, 2014)Hematopoietic stem cells have the capacity to self-renew and give rise to the entirety of the mature blood and immune system throughout the lifespan of an organism. Here, we describe methods to isolate and culture murine ... -
Flow cytometric methods for prenatal and neonatal diagnosis.
(J Immunol Methods, 2011-01-05)Flow cytometry offers a promising alternative to the current methods of amniocentesis or chorionic villus sampling (CVS) for fetal cell sorting for prenatal diagnosis. While flow cytometric methods have been greatly improved ... -
Genome-wide mRNA expression correlates of viral control in CD4+ T-cells from HIV-1-infected individuals.
(PLoS Pathog, 2010-02-26)There is great interindividual variability in HIV-1 viral setpoint after seroconversion, some of which is known to be due to genetic differences among infected individuals. Here, our focus is on determining, genome-wide, ... -
Isolation, purification and in vitro differentiation of cytotrophoblast cells from human term placenta.
(Reproductive biology and endocrinology : RB&E, 2015-07)<h4>Background</h4>The syncytialization of cytotrophoblast cells to syncytiotrophoblast is central to human placental transport and hormone production. Many techniques for in vitro study of this process have been proposed ... -
The liver kinase B1 is a central regulator of T cell development, activation, and metabolism.
(J Immunol, 2011-10-15)T cell activation leads to engagement of cellular metabolic pathways necessary to support cell proliferation and function. However, our understanding of the signal transduction pathways that regulate metabolism and their ...
