Browsing by Subject "Chemistry, Biochemistry"
Now showing items 1-20 of 25
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A Covalent Modification Technique for Protein-Ligand Binding Analysis Using Mass Spectrometry-Based Proteomics Platforms
(2009)Currently there is a dearth of analytical techniques for studying protein-ligand interactions on the proteomic scale. Existing techniques, which rely on various calorimetry or spectroscopy methods, are limited in ... -
A Genetic Screen for the Identification of Mutants Hypersensitive to 5-Azacytidine
(2010)A DNA-protein crosslink is a covalent bond between DNA and a protein. It is a type of DNA damage that is relatively understudied. This study reports on the identification of a set of transposon mutants sensitive to 5-azacytidine, ... -
Analysis and Error Correction in Structures of Macromolecular Interiors and Interfaces
(2009)As of late 2009, the Protein Data Bank (PDB) has grown to contain over 70,000 models. This recent increase in the amount of structural data allows for more extensive explication of the governing principles of macromolecular ... -
Biochemical Characterization of Lipid A Modification Enzymes From Rhizobium leguminosarum and Rhizobium etli
(2010)The lipid A component of lipopolysaccharide (LPS) in the nitrogen-fixing plant endosymbionts <italic>Rhizobium leguminosarum</italic> and <italic>Rhizobium etli</italic> is strikingly different when compared to that of enteric ... -
Biophysical Investigations of Boranophosphate siRNA for Use in RNA Interference against Human Disease
(2009)This project is predicated on the ability of the boranophosphate modification of siRNA to increase its therapeutic applicability for gene silencing in in vitro and in vivo systems. It has been shown that the boranophosphate ... -
Characterization of Peripheral-Membrane Enzymes Required for Lipid A Biosynthesis in Gram-Negative Bacteria
(2010)Gram-negative bacteria possess an asymmetric outer membrane in which the inner leaflet is composed primarily of phospholipids while the outer leaflet contains both phospholipids and lipopolysaccharide (LPS). LPS forms a ... -
Copper at the Interface of Chemistry and Biology: New Insights into hCtr1 Function and the Role of Histidine in Human Cellular Copper Acquisition
(2010)Mechanisms of copper homeostasis are of great interest partly due to their connection to debilitating genetic and neurological disorders. The family of high-affinity copper transporters (Ctr) is responsible for extracellular ... -
Development and Application of a Mass Spectrometry-Based Assay for the High Throughput Analysis of Protein-Ligand Binding
(2009)Many of the biological roles of proteins are modulated through protein-ligand interactions, making proteins important targets for drug therapies and diagnostic imaging probes. The discovery of novel ligands for a protein ... -
In Vivo Analysis of the Consequences and the Repair Mechanisms of Azacytidine-Induced DNA-Protein Crosslinks
(2009)5-azacytidine and its derivatives are cytidine analogs used for leukemia chemotherapy. The primary effect of 5-azacytidine is the prohibition of cytosine methylation, which results in covalent DNA-methyltransferase crosslinks ... -
Interactions of the MetJ Repressor from E. Coli with DNA and SAM
(2009)Transcription regulators are proteins that bind to specific DNA sequences in order to control the expression of specific genes. Often the sequences that are bound are not identical, but contain deviations from a ... -
Ligand Bias by the Endogenous Agonists of CCR7
(2009)Chemokine receptors are members of the seven transmembrane receptor (7TMR) superfamily and are regulated by the G-protein coupled Receptor Kinase (GRK)/ b-arrestin system. CCL19 and CCL21 are endogenous agonists for the ... -
Molecular Bioengineering: From Protein Stability to Population Suicide
(2010)Driven by the development of new technologies and an ever expanding knowledge base of molecular and cellular function, Biology is rapidly gaining the potential to develop into a veritable engineering discipline - the so-called ... -
Molecular Computing with DNA Self-Assembly
(2009)Synthetic biology is an emerging technology field whose goal is to use biology as a substrate for engineering. Self-assembly is one of the many methods for fabricating such synthetic biosystems.Self-assembly is a process ... -
Nitric Oxide in Health and Disease: Physiology, Pathophysiology, and Clinical Measurement
(2008-12-11)Red blood cell-dependent hypoxic vasodilation is largely mediated via the delivery of NO through S-nitrosohemoglobin (Hb-SNO). Hb-SNO is regulated through allosteric and redox mechanisms that are not well understood. Part ... -
NMR Structure Improvement: A Structural Bioinformatics & Visualization Approach
(2010)The overall goal of this project is to enhance the physical accuracy of individual models in macromolecular NMR (Nuclear Magnetic Resonance) structures and the realism of variation within NMR ensembles of models, while improving ... -
Protein Engineering for Biosensor Development
(2008-11-24)Biosensors incorporating proteins as molecular recognition elements for analytes are used in clinical diagnostics, as biological research tools, and to detect chemical threats and pollutants. This work describes the application ... -
Protein S-nitrosylation: Methods of Detection and Regulation
(2009)Protein S-nitrosylation--the post-translational modification of cysteine thiols into S-nitrosothiols--is a principle mechanism of nitric oxide-based signaling. Studies have demonstrated myriad roles for S-nitrosylation in ... -
RNA Backbone Rotamers and Chiropraxis
(2007-07-25)RNA backbone is biologically important with many roles in reactions and interactions, but has historically been a challenge in structural determination. It has many atoms and torsions to place, and often there is less data ... -
Structural Biochemistry and Inhibition of CaaX Protein Prenyltransferases From Human Pathogens
(2010)Protein prenylation is a post-translational lipid modification required for proper function by over 100 proteins in the eukaryotic cell. Proteins that receive this modification mediate a wide variety of functions in the ... -
Structural Determinants of Post-transcriptional Protein Regulation as Modulators of Monoamine Signaling
(2008-06-23)Monoamines were first discovered at the end of the 19th century when William Bates identified epinephrine (EPI) and noted its hemostatic effects. During the 20th century, norepinephrine (NE), dopamine (DA), and serotonin ...
