Browsing by Subject "Consensus Sequence"
Now showing items 1-4 of 4
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Binding of MetJ repressor to specific and nonspecific DNA and effect of S-adenosylmethionine on these interactions.
(Biochemistry, 2010-04-20)We have used analytical ultracentrifugation to characterize the binding of the methionine repressor protein, MetJ, to synthetic oligonucleotides containing zero to five specific recognition sites, called metboxes. For all ... -
Next-generation polyploid phylogenetics: rapid resolution of hybrid polyploid complexes using PacBio single-molecule sequencing.
(The New phytologist, 2017-01)Difficulties in generating nuclear data for polyploids have impeded phylogenetic study of these groups. We describe a high-throughput protocol and an associated bioinformatics pipeline (Pipeline for Untangling Reticulate ... -
Similar T-cell immune responses induced by group M consensus env immunogens with wild-type or minimum consensus variable regions.
(AIDS Res Hum Retroviruses, 2010-05)Consensus HIV-1 genes can decrease the genetic distances between candidate immunogens and field virus strains. To ensure the functionality and optimal presentation of immunologic epitopes, we generated two group-M consensus ... -
Transmission of single HIV-1 genomes and dynamics of early immune escape revealed by ultra-deep sequencing.
(PLoS One, 2010-08-20)We used ultra-deep sequencing to obtain tens of thousands of HIV-1 sequences from regions targeted by CD8+ T lymphocytes from longitudinal samples from three acutely infected subjects, and modeled viral evolution during ...
