Now showing items 1-14 of 14

    • A kinesin motor in a force-producing conformation. 

      Heuston, E; Bronner, CE; Kull, FJ; Endow, SA (BMC Struct Biol, 2010-07-05)
      BACKGROUND: Kinesin motors hydrolyze ATP to produce force and move along microtubules, converting chemical energy into work by a mechanism that is only poorly understood. Key transitions and intermediate states in the process ...
    • Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus. 

      Alam, S Munir; Boyd, SD; Cai, Fangping; Chen, S; Chen, Y; Cohen, M; Du, X; ... (45 authors) (Nature, 2013-04-25)
      Current human immunodeficiency virus-1 (HIV-1) vaccines elicit strain-specific neutralizing antibodies. However, cross-reactive neutralizing antibodies arise in approximately 20% of HIV-1-infected individuals, and details ...
    • Competition between monomeric and dimeric crystals in schematic models for globular proteins. 

      Charbonneau, Patrick; Fusco, Diana (J Phys Chem B, 2014-07-17)
      Advances in experimental techniques and in theoretical models have improved our understanding of protein crystallization. However, they have also left open questions regarding the protein phase behavior and self-assembly ...
    • Computational Methods for RNA Structure Validation and Improvement. 

      Jain, S; Richardson, DC; Richardson, JS (Methods Enzymol, 2015)
      With increasing recognition of the roles RNA molecules and RNA/protein complexes play in an unexpected variety of biological processes, understanding of RNA structure-function relationships is of high current importance. ...
    • Drug design from the cryptic inhibitor envelope. 

      Gopalaswamy, R; Lee, CJ; Lemaître, N; Liang, X; Najeeb, Javaria; Sebbane, F; Titecat, M; ... (11 authors) (Nat Commun, 2016-02-25)
      Conformational dynamics plays an important role in enzyme catalysis, allosteric regulation of protein functions and assembly of macromolecular complexes. Despite these well-established roles, such information has yet to ...
    • Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions. 

      Wu, S; Liu, J; Reedy, MC; Tregear, RT; Winkler, H; Franzini-Armstrong, C; Sasaki, H; ... (11 authors) (PLoS One, 2010-09-09)
      BACKGROUND: Isometric muscle contraction, where force is generated without muscle shortening, is a molecular traffic jam in which the number of actin-attached motors is maximized and all states of motor action are trapped ...
    • FtsZ filament capping by MciZ, a developmental regulator of bacterial division. 

      Bisson-Filho, Alexandre W; Blasios, Valdir; Castellen, Patrícia; Dessen, Andréa; Discola, Karen F; Erickson, Harold Paul; Garcia, Wanius; ... (11 authors) (Proceedings of the National Academy of Sciences of the United States of America, 2015-04-06)
      Cytoskeletal structures are dynamically remodeled with the aid of regulatory proteins. FtsZ (filamentation temperature-sensitive Z) is the bacterial homolog of tubulin that polymerizes into rings localized to cell-division ...
    • Multiscale conformational heterogeneity in staphylococcal protein a: possible determinant of functional plasticity. 

      Deis, LN; Hagarman, A; Oas, Terrence Gilbert; Pemble IV, Charles W; Qi, Y; Richardson, DC; Richardson, JS (Structure, 2014-10-07)
      The Staphylococcus aureus virulence factor staphylococcal protein A (SpA) is a major contributor to bacterial evasion of the host immune system, through high-affinity binding to host proteins such as antibodies. SpA includes ...
    • New insights into Hoogsteen base pairs in DNA duplexes from a structure-based survey. 

      Zhou, H; Hintze, BJ; Kimsey, IJ; Sathyamoorthy, B; Yang, S; Richardson, JS; Al-Hashimi, HM (Nucleic Acids Res, 2015-04-20)
      Hoogsteen (HG) base pairs (bps) provide an alternative pairing geometry to Watson-Crick (WC) bps and can play unique functional roles in duplex DNA. Here, we use structural features unique to HG bps (syn purine base, HG ...
    • Statistical analysis of crystallization database links protein physico-chemical features with crystallization mechanisms. 

      Barnum, TJ; Bruno, AE; Charbonneau, Patrick; Fusco, Diana; Luft, JR; Mukherjee, Sayan; Snell, EH (PLoS One, 2014)
      X-ray crystallography is the predominant method for obtaining atomic-scale information about biological macromolecules. Despite the success of the technique, obtaining well diffracting crystals still critically limits going ...
    • Structural and Functional Analysis of the GADD34:PP1 eIF2α Phosphatase. 

      Choy, Meng S; Goh, Catherine W; Lee, Irene C; Newton, Jocelyn C; Page, Rebecca; Peti, Wolfgang; Shenolikar, Shirish; ... (8 authors) (Cell reports, 2015-06-18)
      The attenuation of protein synthesis via the phosphorylation of eIF2α is a major stress response of all eukaryotic cells. The growth-arrest- and DNA-damage-induced transcript 34 (GADD34) bound to the serine/threonine protein ...
    • Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9. 

      Arthos, J; Bewley, CA; Bonsignori, Mattia; Boyington, JC; Burton, DR; Carrico, C; Chuang, G-Y; ... (47 authors) (Nature, 2011-11-23)
      Variable regions 1 and 2 (V1/V2) of human immunodeficiency virus-1 (HIV-1) gp120 envelope glycoprotein are critical for viral evasion of antibody neutralization, and are themselves protected by extraordinary sequence diversity ...
    • Suppression of conformational heterogeneity at a protein-protein interface. 

      Daniels, Kyle G; Deis, LN; Oas, Terrence Gilbert; Qi, Y; Wang, Y; Wu, Q; Zhou, Pei (Proc Natl Acad Sci U S A, 2015-07-21)
      Staphylococcal protein A (SpA) is an important virulence factor from Staphylococcus aureus responsible for the bacterium's evasion of the host immune system. SpA includes five small three-helix-bundle domains that can each ...
    • The structure of irisin reveals a novel intersubunit β-sheet fibronectin type III (FNIII) dimer: implications for receptor activation. 

      Chinnam, Nagababu; Erickson, Harold Paul; Ohashi, Tomoo; Schumacher, Maria; Shah, Riddhi Sanjay (The Journal of biological chemistry, 2013-11)
      Irisin was recently identified as a putative myokine that is induced by exercise. Studies suggest that it is produced by cleavage of the FNDC5 (fibronectin domain-containing protein 5) receptor; irisin corresponds to the ...