Now showing items 1-14 of 14

    • A kinesin motor in a force-producing conformation. 

      Heuston, Elisabeth; Bronner, C Eric; Kull, F Jon; Endow, Sharyn A (BMC Struct Biol, 2010-07-05)
      BACKGROUND: Kinesin motors hydrolyze ATP to produce force and move along microtubules, converting chemical energy into work by a mechanism that is only poorly understood. Key transitions and intermediate states in the process ...
    • Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus. 

      Liao, Hua-Xin; Lynch, Rebecca; Zhou, Tongqing; Gao, Feng; Alam, S Munir; Boyd, Scott D; Fire, Andrew Z; ... (45 authors) (Nature, 2013-04-25)
      Current human immunodeficiency virus-1 (HIV-1) vaccines elicit strain-specific neutralizing antibodies. However, cross-reactive neutralizing antibodies arise in approximately 20% of HIV-1-infected individuals, and details ...
    • Competition between monomeric and dimeric crystals in schematic models for globular proteins. 

      Fusco, Diana; Charbonneau, Patrick (J Phys Chem B, 2014-07-17)
      Advances in experimental techniques and in theoretical models have improved our understanding of protein crystallization. However, they have also left open questions regarding the protein phase behavior and self-assembly ...
    • Computational Methods for RNA Structure Validation and Improvement. 

      Jain, Swati; Richardson, David C; Richardson, Jane S (Methods Enzymol, 2015)
      With increasing recognition of the roles RNA molecules and RNA/protein complexes play in an unexpected variety of biological processes, understanding of RNA structure-function relationships is of high current importance. ...
    • Drug design from the cryptic inhibitor envelope. 

      Lee, Chul-Jin; Liang, Xiaofei; Wu, Qinglin; Najeeb, Javaria; Zhao, Jinshi; Gopalaswamy, Ramesh; Titecat, Marie; ... (11 authors) (Nat Commun, 2016-02-25)
      Conformational dynamics plays an important role in enzyme catalysis, allosteric regulation of protein functions and assembly of macromolecular complexes. Despite these well-established roles, such information has yet to ...
    • Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions. 

      Wu, Shenping; Liu, Jun; Reedy, Mary C; Tregear, Richard T; Winkler, Hanspeter; Franzini-Armstrong, Clara; Sasaki, Hiroyuki; ... (11 authors) (PLoS One, 2010-09-09)
      BACKGROUND: Isometric muscle contraction, where force is generated without muscle shortening, is a molecular traffic jam in which the number of actin-attached motors is maximized and all states of motor action are trapped ...
    • FtsZ filament capping by MciZ, a developmental regulator of bacterial division. 

      Bisson-Filho, Alexandre W; Discola, Karen F; Castellen, Patrícia; Blasios, Valdir; Martins, Alexandre; Sforça, Maurício L; Garcia, Wanius; ... (11 authors) (Proceedings of the National Academy of Sciences of the United States of America, 2015-04-06)
      Cytoskeletal structures are dynamically remodeled with the aid of regulatory proteins. FtsZ (filamentation temperature-sensitive Z) is the bacterial homolog of tubulin that polymerizes into rings localized to cell-division ...
    • Multiscale conformational heterogeneity in staphylococcal protein a: possible determinant of functional plasticity. 

      Deis, LN; Hagarman, A; Oas, Terrence Gilbert; Pemble IV, Charles W; Qi, Y; Richardson, DC; Richardson, JS (Structure, 2014-10-07)
      The Staphylococcus aureus virulence factor staphylococcal protein A (SpA) is a major contributor to bacterial evasion of the host immune system, through high-affinity binding to host proteins such as antibodies. SpA includes ...
    • New insights into Hoogsteen base pairs in DNA duplexes from a structure-based survey. 

      Zhou, Huiqing; Hintze, Bradley J; Kimsey, Isaac J; Sathyamoorthy, Bharathwaj; Yang, Shan; Richardson, Jane S; Al-Hashimi, Hashim M (Nucleic Acids Res, 2015-04-20)
      Hoogsteen (HG) base pairs (bps) provide an alternative pairing geometry to Watson-Crick (WC) bps and can play unique functional roles in duplex DNA. Here, we use structural features unique to HG bps (syn purine base, HG ...
    • Statistical analysis of crystallization database links protein physico-chemical features with crystallization mechanisms. 

      Fusco, Diana; Barnum, Timothy J; Bruno, Andrew E; Luft, Joseph R; Snell, Edward H; Mukherjee, Sayan; Charbonneau, Patrick (PLoS One, 2014)
      X-ray crystallography is the predominant method for obtaining atomic-scale information about biological macromolecules. Despite the success of the technique, obtaining well diffracting crystals still critically limits going ...
    • Structural and Functional Analysis of the GADD34:PP1 eIF2α Phosphatase. 

      Choy, Meng S; Yusoff, Permeen; Lee, Irene C; Newton, Jocelyn C; Goh, Catherine W; Page, Rebecca; Shenolikar, Shirish; ... (8 authors) (Cell reports, 2015-06-18)
      The attenuation of protein synthesis via the phosphorylation of eIF2α is a major stress response of all eukaryotic cells. The growth-arrest- and DNA-damage-induced transcript 34 (GADD34) bound to the serine/threonine protein ...
    • Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9. 

      McLellan, Jason S; Pancera, Marie; Carrico, Chris; Gorman, Jason; Julien, Jean-Philippe; Khayat, Reza; Louder, Robert; ... (47 authors) (Nature, 2011-11-23)
      Variable regions 1 and 2 (V1/V2) of human immunodeficiency virus-1 (HIV-1) gp120 envelope glycoprotein are critical for viral evasion of antibody neutralization, and are themselves protected by extraordinary sequence diversity ...
    • Suppression of conformational heterogeneity at a protein-protein interface. 

      Deis, Lindsay N; Wu, Qinglin; Wang, You; Qi, Yang; Daniels, Kyle G; Zhou, Pei; Oas, Terrence G (Proc Natl Acad Sci U S A, 2015-07-21)
      Staphylococcal protein A (SpA) is an important virulence factor from Staphylococcus aureus responsible for the bacterium's evasion of the host immune system. SpA includes five small three-helix-bundle domains that can each ...
    • The structure of irisin reveals a novel intersubunit β-sheet fibronectin type III (FNIII) dimer: implications for receptor activation. 

      Schumacher, Maria A; Chinnam, Nagababu; Ohashi, Tomoo; Shah, Riddhi Sanjay; Erickson, Harold P (The Journal of biological chemistry, 2013-11)
      Irisin was recently identified as a putative myokine that is induced by exercise. Studies suggest that it is produced by cleavage of the FNDC5 (fibronectin domain-containing protein 5) receptor; irisin corresponds to the ...