Now showing items 1-16 of 16

    • Cell division without FtsZ--a variety of redundant mechanisms. 

      Erickson, Harold Paul; Osawa, Masaki (Molecular microbiology, 2010-10)
      Until 1998 it looked like all bacteria and archaea used a universal cytokinetic machine based on FtsZ. A dozen completely sequenced bacterial genomes all had an ftsZ gene, as did the several sequenced archaeal genomes. Then ...
    • Conformational changes of FtsZ reported by tryptophan mutants. 

      Chen, Yaodong; Erickson, Harold Paul (Biochemistry, 2011-05-03)
      E. coli FtsZ has no native tryptophan. We showed previously that the mutant FtsZ L68W gave a 2.5-fold increase in trp fluorescence when assembly was induced by GTP. L68 is probably buried in the protofilament interface upon ...
    • Consensus nomenclature for the human ArfGAP domain-containing proteins. 

      Bruford, E; Cassel, D; Inoue, H; Kahn, RA; Logsdon, JM; Nie, Z; Premont, Richard Thomas; ... (11 authors) (J Cell Biol, 2008-09-22)
      At the FASEB summer research conference on "Arf Family GTPases", held in Il Ciocco, Italy in June, 2007, it became evident to researchers that our understanding of the family of Arf GTPase activating proteins (ArfGAPs) has ...
    • Dynamics of PTH-induced disassembly of Npt2a/NHERF-1 complexes in living OK cells. 

      Blanpied, Thomas A; Shenolikar, Shirish; Steplock, Deborah; Weinman, Edward J (American journal of physiology. Renal physiology, 2011-01)
      Parathyroid hormone (PTH) inhibits the reabsorption of phosphate in the renal proximal tubule by disrupting the binding of the sodium-dependent phosphate transporter 2A (Npt2a) to the adapter protein sodium-hydrogen exchanger ...
    • FtsZ filament capping by MciZ, a developmental regulator of bacterial division. 

      Bisson-Filho, Alexandre W; Blasios, Valdir; Castellen, Patrícia; Dessen, Andréa; Discola, Karen F; Erickson, Harold Paul; Garcia, Wanius; ... (11 authors) (Proceedings of the National Academy of Sciences of the United States of America, 2015-04-06)
      Cytoskeletal structures are dynamically remodeled with the aid of regulatory proteins. FtsZ (filamentation temperature-sensitive Z) is the bacterial homolog of tubulin that polymerizes into rings localized to cell-division ...
    • FtsZ in bacterial cytokinesis: cytoskeleton and force generator all in one. 

      Anderson, David E; Erickson, Harold Paul; Osawa, Masaki (Microbiology and molecular biology reviews : MMBR, 2010-12)
      FtsZ, a bacterial homolog of tubulin, is well established as forming the cytoskeletal framework for the cytokinetic ring. Recent work has shown that purified FtsZ, in the absence of any other division proteins, can assemble ...
    • FtsZ Protofilament Curvature Is the Opposite of Tubulin Rings. 

      Erickson, Harold Paul; Housman, Max; Milam, Sara L; Moore, Desmond A; Osawa, Masaki (Biochemistry, 2016-07)
      FtsZ protofilaments (pfs) form the bacterial cytokinetic Z ring. Previous work suggested that a conformational change from straight to curved pfs generated the constriction force. In the simplest model, the C-terminal membrane ...
    • Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division. 

      Erickson, Harold Paul; Kiro, Ruth; Milam, Sara L; Molshanski-Mor, Shahar; Qimron, Udi; Yosef, Ido (Proceedings of the National Academy of Sciences of the United States of America, 2013-11-11)
      Bacteriophages take over host resources primarily via the activity of proteins expressed early in infection. One of these proteins, produced by the Escherichia coli phage T7, is gene product (Gp) 0.4. Here, we show that ...
    • Inside-out Z rings--constriction with and without GTP hydrolysis. 

      Erickson, Harold Paul; Osawa, Masaki (Molecular microbiology, 2011-07)
      The bacterial tubulin homologue FtsZ forms a ring-like structure called the Z ring that drives cytokinesis. We showed previously that FtsZ-YFP-mts, which has a short amphipathic helix (mts) on its C terminus that inserts ...
    • Liposome division by a simple bacterial division machinery. 

      Erickson, Harold Paul; Osawa, Masaki (Proceedings of the National Academy of Sciences of the United States of America, 2013-07)
      We previously reconstituted Z rings in tubular multilamellar liposomes with FtsZ-YFP-mts, where mts is a membrane-targeting amphiphilic helix. These reconstituted Z rings generated a constriction force but did not divide ...
    • LKB1 Loss induces characteristic patterns of gene expression in human tumors associated with NRF2 activation and attenuation of PI3K-AKT. 

      Kaufman, Jacob; Amann, Joseph M; Park, Kyungho; Arasada, Rajeswara Rao; Li, Haotian; Shyr, Yu; Carbone, David P (Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, 2014-06)
      Inactivation of serine/threonine kinase 11 (STK11 or LKB1) is common in lung cancer, and understanding the pathways and phenotypes altered as a consequence will aid the development of targeted therapeutic strategies. Gene ...
    • Negative-stain electron microscopy of inside-out FtsZ rings reconstituted on artificial membrane tubules show ribbons of protofilaments. 

      Erickson, Harold Paul; Milam, Sara L; Osawa, Masaki (Biophysical journal, 2012-07)
      FtsZ, the primary cytoskeletal element of the Z ring, which constricts to divide bacteria, assembles into short, one-stranded filaments in vitro. These must be further assembled to make the Z ring in bacteria. Conventional ...
    • Rapid in vitro assembly of Caulobacter crescentus FtsZ protein at pH 6.5 and 7.2. 

      Erickson, Harold Paul; Milam, Sara L (The Journal of biological chemistry, 2013-08)
      FtsZ from most bacteria assembles rapidly in vitro, reaching a steady-state plateau in 5-10 s after addition of GTP. A recent study used a novel dynamic light-scattering technique to assay the assembly of FtsZ from Caulobacter ...
    • Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis. 

      Chang, JS; Chi, RJ; Lansley, T; Lemmon, Sandra K; Newpher, Thomas Mark; Segarra, VA; Torres, OT (J Cell Sci, 2012-10-15)
      Phosphorylation regulates assembly and disassembly of proteins during endocytosis. In yeast, Prk1 and Ark1 phosphorylate factors after vesicle internalization leading to coat disassembly. Scd5, a protein phosphatase-1 ...
    • SulA inhibits assembly of FtsZ by a simple sequestration mechanism. 

      Chen, Yaodong; Erickson, Harold Paul; Milam, Sara L (Biochemistry, 2012-04)
      We have investigated the inhibition by SulA of the assembly of Escherichia coli FtsZ. Using quantitative GTPase and fluorescence assays, we found that SulA inhibition resulted in an increase in the apparent critical concentration ...
    • The C-terminal linker of Escherichia coli FtsZ functions as an intrinsically disordered peptide. 

      Erickson, Harold Paul; Gardner, Kiani AJ Arkus; Moore, Desmond A (Molecular microbiology, 2013-07)
      The tubulin homologue FtsZ provides the cytoskeletal framework and constriction force for bacterial cell division. FtsZ has an 50-amino-acid (aa) linker between the protofilament-forming globular domain and the C-terminal ...