Browsing by Subject "Cytoskeletal Proteins"
Now showing items 1-20 of 26
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Cell division without FtsZ--a variety of redundant mechanisms.
(Molecular microbiology, 2010-10)Until 1998 it looked like all bacteria and archaea used a universal cytokinetic machine based on FtsZ. A dozen completely sequenced bacterial genomes all had an ftsZ gene, as did the several sequenced archaeal genomes. Then ... -
Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings.
(Methods in enzymology, 2009-01)We have developed a system for producing tubular multilamellar liposomes that incorporate the protein FtsZ on the inside. We start with a mixture of spherical multilamellar liposomes with FtsZ initially on the outside. Shearing ... -
Clathrin is important for normal actin dynamics and progression of Sla2p-containing patches during endocytosis in yeast.
(Traffic (Copenhagen, Denmark), 2006-05)Clathrin is a major vesicle coat protein involved in receptor-mediated endocytosis. In yeast and higher eukaryotes, clathrin is recruited to the plasma membrane during the early stage of endocytosis along with clathrin-associated ... -
Conformational changes of FtsZ reported by tryptophan mutants.
(Biochemistry, 2011-05-03)E. coli FtsZ has no native tryptophan. We showed previously that the mutant FtsZ L68W gave a 2.5-fold increase in trp fluorescence when assembly was induced by GTP. L68 is probably buried in the protofilament interface upon ... -
Consensus nomenclature for the human ArfGAP domain-containing proteins.
(J Cell Biol, 2008-09-22)At the FASEB summer research conference on "Arf Family GTPases", held in Il Ciocco, Italy in June, 2007, it became evident to researchers that our understanding of the family of Arf GTPase activating proteins (ArfGAPs) has ... -
Curved FtsZ protofilaments generate bending forces on liposome membranes.
(The EMBO journal, 2009-11)We have created FtsZ-YFP-mts where an amphipathic helix on the C-terminus tethers FtsZ to the membrane. When incorporated inside multi-lamellar tubular liposomes, FtsZ-YFP-mts can assemble Z rings that generate a constriction ... -
Dynamics of PTH-induced disassembly of Npt2a/NHERF-1 complexes in living OK cells.
(American journal of physiology. Renal physiology, 2011-01)Parathyroid hormone (PTH) inhibits the reabsorption of phosphate in the renal proximal tubule by disrupting the binding of the sodium-dependent phosphate transporter 2A (Npt2a) to the adapter protein sodium-hydrogen exchanger ... -
FtsZ at mid-cell is essential in Escherichia coli until the late stage of constriction.
(Microbiology (Reading, England), 2022-06)There has been recent debate as to the source of constriction force during cell division. FtsZ can generate a constriction force on tubular membranes in vitro, suggesting it may generate the constriction force in vivo. However, ... -
FtsZ filament capping by MciZ, a developmental regulator of bacterial division.
(Proceedings of the National Academy of Sciences of the United States of America, 2015-04-06)Cytoskeletal structures are dynamically remodeled with the aid of regulatory proteins. FtsZ (filamentation temperature-sensitive Z) is the bacterial homolog of tubulin that polymerizes into rings localized to cell-division ... -
FtsZ in bacterial cytokinesis: cytoskeleton and force generator all in one.
(Microbiology and molecular biology reviews : MMBR, 2010-12)FtsZ, a bacterial homolog of tubulin, is well established as forming the cytoskeletal framework for the cytokinetic ring. Recent work has shown that purified FtsZ, in the absence of any other division proteins, can assemble ... -
FtsZ Protofilament Curvature Is the Opposite of Tubulin Rings.
(Biochemistry, 2016-07)FtsZ protofilaments (pfs) form the bacterial cytokinetic Z ring. Previous work suggested that a conformational change from straight to curved pfs generated the constriction force. In the simplest model, the C-terminal membrane ... -
Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division.
(Proceedings of the National Academy of Sciences of the United States of America, 2013-11-11)Bacteriophages take over host resources primarily via the activity of proteins expressed early in infection. One of these proteins, produced by the Escherichia coli phage T7, is gene product (Gp) 0.4. Here, we show that ... -
High-resolution crystal structures of Escherichia coli FtsZ bound to GDP and GTP.
(Acta crystallographica. Section F, Structural biology communications, 2020-02-05)Bacterial cytokinesis is mediated by the Z-ring, which is formed by the prokaryotic tubulin homolog FtsZ. Recent data indicate that the Z-ring is composed of small patches of FtsZ protofilaments that travel around the bacterial ... -
How cells determine the number of polarity sites.
(eLife, 2021-04-26)The diversity of cell morphologies arises, in part, through regulation of cell polarity by Rho-family GTPases. A poorly understood but fundamental question concerns the regulatory mechanisms by which different cells generate ... -
In vivo dynamics of clathrin and its adaptor-dependent recruitment to the actin-based endocytic machinery in yeast.
(Developmental cell, 2005-07)Clathrin-mediated transport is a major pathway for endocytosis. However, in yeast, where cortical actin patches are essential for endocytosis, plasma membrane-associated clathrin has never been observed. Using live cell ... -
Inside-out Z rings--constriction with and without GTP hydrolysis.
(Molecular microbiology, 2011-07)The bacterial tubulin homologue FtsZ forms a ring-like structure called the Z ring that drives cytokinesis. We showed previously that FtsZ-YFP-mts, which has a short amphipathic helix (mts) on its C terminus that inserts ... -
Liposome division by a simple bacterial division machinery.
(Proceedings of the National Academy of Sciences of the United States of America, 2013-07)We previously reconstituted Z rings in tubular multilamellar liposomes with FtsZ-YFP-mts, where mts is a membrane-targeting amphiphilic helix. These reconstituted Z rings generated a constriction force but did not divide ... -
LKB1 Loss induces characteristic patterns of gene expression in human tumors associated with NRF2 activation and attenuation of PI3K-AKT.
(Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, 2014-06)Inactivation of serine/threonine kinase 11 (STK11 or LKB1) is common in lung cancer, and understanding the pathways and phenotypes altered as a consequence will aid the development of targeted therapeutic strategies. Gene ... -
Mutations in NCAPG2 Cause a Severe Neurodevelopmental Syndrome that Expands the Phenotypic Spectrum of Condensinopathies.
(American journal of human genetics, 2019-01)The use of whole-exome and whole-genome sequencing has been a catalyst for a genotype-first approach to diagnostics. Under this paradigm, we have implemented systematic sequencing of neonates and young children with a suspected ... -
Negative-stain electron microscopy of inside-out FtsZ rings reconstituted on artificial membrane tubules show ribbons of protofilaments.
(Biophysical journal, 2012-07)FtsZ, the primary cytoskeletal element of the Z ring, which constricts to divide bacteria, assembles into short, one-stranded filaments in vitro. These must be further assembled to make the Z ring in bacteria. Conventional ...
