Browsing by Subject "Escherichia coli"
Now showing items 1-20 of 37
-
A noisy linear map underlies oscillations in cell size and gene expression in bacteria.
(Nature, 2015-07-16)During bacterial growth, a cell approximately doubles in size before division, after which it splits into two daughter cells. This process is subjected to the inherent perturbations of cellular noise and thus requires regulation ... -
Bacterial effector activates jasmonate signaling by directly targeting JAZ transcriptional repressors.
(PLoS pathogens, 2013-10-31)Gram-negative bacterial pathogens deliver a variety of virulence proteins through the type III secretion system (T3SS) directly into the host cytoplasm. These type III secreted effectors (T3SEs) play an essential role in ... -
Biochemical and structural mechanisms of multidrug efflux pump transcription regulators, Neisseria gonorrhoeae MtrR and Escherichia coli MprA
(2021)As bacterial resistance to multiple antibiotics continues to become a growing problem across the globe, the imperativeness for understanding mechanisms of antibiotic and multidrug resistance is increasingly apparent. Currently, ... -
Bloodstream infections in community hospitals in the 21st century: a multicenter cohort study.
(PLoS One, 2014)BACKGROUND: While the majority of healthcare in the US is provided in community hospitals, the epidemiology and treatment of bloodstream infections in this setting is unknown. METHODS AND FINDINGS: We undertook this multicenter, ... -
Contribution of urinary tract infection to the burden of febrile illnesses in young children in rural Kenya.
(PLoS One, 2017)INTRODUCTION: The clinical features of UTI in young children may not localize to the urinary tract and closely resemble other febrile illnesses. In malaria endemic areas, a child presenting with fever is often treated ... -
Differential coordination demands in Fe versus Mn water-soluble cationic metalloporphyrins translate into remarkably different aqueous redox chemistry and biology.
(Inorganic chemistry, 2013-05-06)The different biological behavior of cationic Fe and Mn pyridylporphyrins in Escherichia coli and mouse studies prompted us to revisit and compare their chemistry. For that purpose, the series of ortho and meta isomers of ... -
Differential inhibition of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and TZM-bl cells by endotoxin-mediated chemokine and gamma interferon production.
(AIDS Res Hum Retroviruses, 2010-03)Bacterial lipopolysaccharide (endotoxin) is a frequent contaminant of biological specimens and is also known to be a potent inducer of beta-chemokines and other soluble factors that inhibit HIV-1 infection in vitro. Though ... -
Discovery of the Elusive UDP-Diacylglucosamine Hydrolase in the Lipid A Biosynthetic Pathway in Chlamydia trachomatis.
(MBio, 2016-03-22)Constitutive biosynthesis of lipid A via the Raetz pathway is essential for the viability and fitness of Gram-negative bacteria, includingChlamydia trachomatis Although nearly all of the enzymes in the lipid A biosynthetic ... -
Drug design from the cryptic inhibitor envelope.
(Nat Commun, 2016-02-25)Conformational dynamics plays an important role in enzyme catalysis, allosteric regulation of protein functions and assembly of macromolecular complexes. Despite these well-established roles, such information has yet to ... -
Epigenetic regulation of the nitrosative stress response and intracellular macrophage survival by extraintestinal pathogenic Escherichia coli.
(2011)Escherichia coli is a typical constituent of the enteric tract in many animals, including humans. However, specialized extraintestinal pathogenic E. colistrains (ExPEC) may transition from benign occupation of the enteric ... -
Escherichia coli global gene expression in urine from women with urinary tract infection.
(PLoS pathogens, 2010-11)Murine models of urinary tract infection (UTI) have provided substantial data identifying uropathogenic E. coli (UPEC) virulence factors and assessing their expression in vivo. However, it is unclear how gene expression ... -
FtsZ at mid-cell is essential in Escherichia coli until the late stage of constriction.
(Microbiology (Reading, England), 2022-06)There has been recent debate as to the source of constriction force during cell division. FtsZ can generate a constriction force on tubular membranes in vitro, suggesting it may generate the constriction force in vivo. However, ... -
Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division.
(Proceedings of the National Academy of Sciences of the United States of America, 2013-11-11)Bacteriophages take over host resources primarily via the activity of proteins expressed early in infection. One of these proteins, produced by the Escherichia coli phage T7, is gene product (Gp) 0.4. Here, we show that ... -
Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.
(PLoS One, 2015)The production of outer membrane vesicles by Gram-negative bacteria has been well documented; however, the mechanism behind the biogenesis of these vesicles remains unclear. Here a high-throughput experimental method and ... -
Heterogeneities in fullerene nanoparticle aggregates affecting reactivity, bioactivity, and transport.
(ACS Nano, 2010-09-28)Properties of nanomaterial suspensions are typically summarized by average values for the purposes of characterizing these materials and interpreting experimental results. We show in this work that the heterogeneity in aqueous ... -
High-resolution crystal structures of Escherichia coli FtsZ bound to GDP and GTP.
(Acta crystallographica. Section F, Structural biology communications, 2020-02-05)Bacterial cytokinesis is mediated by the Z-ring, which is formed by the prokaryotic tubulin homolog FtsZ. Recent data indicate that the Z-ring is composed of small patches of FtsZ protofilaments that travel around the bacterial ... -
High-throughput identification of chemical inhibitors of E. coli Group 2 capsule biogenesis as anti-virulence agents.
(PLoS One, 2010-07-19)Rising antibiotic resistance among Escherichia coli, the leading cause of urinary tract infections (UTIs), has placed a new focus on molecular pathogenesis studies, aiming to identify new therapeutic targets. Anti-virulence ... -
High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection.
(PLoS One, 2008-08-06)We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation ... -
L form bacteria growth in low-osmolality medium.
(Microbiology (Reading, England), 2019-08)L form bacteria do not have a cell wall and are thought to require medium of high osmolality for survival and growth. In this study we tested whether L forms can adapt to growth in lower osmolality medium. We first tested ... -
Methoxy-derivatization of alkyl chains increases the in vivo efficacy of cationic Mn porphyrins. Synthesis, characterization, SOD-like activity, and SOD-deficient E. coli study of meta Mn(III) N-methoxyalkylpyridylporphyrins.
(Dalton transactions (Cambridge, England : 2003), 2011-04)Cationic Mn(III) N-alkylpyridylporphyrins (MnPs) are potent SOD mimics and peroxynitrite scavengers and diminish oxidative stress in a variety of animal models of central nervous system (CNS) injuries, cancer, radiation, ...
