Browsing by Subject "Flow Cytometry"
Now showing items 1-20 of 39
-
A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines.
(Communications biology, 2021-11)Multiplex immunoassays with acellular antigens are well-established based on solid-phase platforms such as the Luminex® technology. Cell barcoding by amine-reactive fluorescent dyes enables analogous cell-based multiplex ... -
A discrete chromatin loop in the mouse Tcra-Tcrd locus shapes the TCRδ and TCRα repertoires.
(Nat Immunol, 2015-10)The locus encoding the T cell antigen receptor (TCR) α-chain and δ-chain (Tcra-Tcrd) undergoes recombination of its variable-diversity-joining (V(D)J) segments in CD4(-)CD8(-) double-negative thymocytes and CD4(+)CD8(+) ... -
A model for harmonizing flow cytometry in clinical trials.
(Nat Immunol, 2010-11)Complexities in sample handling, instrument setup and data analysis are barriers to the effective use of flow cytometry to monitor immunological parameters in clinical trials. The novel use of a central laboratory may help ... -
A novel, non-apoptotic role for Scythe/BAT3: a functional switch between the pro- and anti-proliferative roles of p21 during the cell cycle.
(2012)Scythe/BAT3 is a member of the BAG protein family whose role in apoptosis, a form of programmed cell death, has been extensively studied. However, since the developmental defects observed in Bat3‐null mouse embryos cannot ... -
A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflamed Murine Non-Lymphoid Tissues.
(PloS one, 2016-01)Flow cytometry is used extensively to examine immune cells in non-lymphoid tissues. However, a method of flow cytometric analysis that is both comprehensive and widely applicable has not been described. We developed a protocol ... -
Airway fibroblasts in asthma manifest an invasive phenotype.
(American journal of respiratory and critical care medicine, 2011-06)<h4>Rationale</h4>Invasive cell phenotypes have been demonstrated in malignant transformation, but not in other diseases, such as asthma. Cellular invasiveness is thought to be mediated by transforming growth factor (TGF)-β1 ... -
Boosting high-intensity focused ultrasound-induced anti-tumor immunity using a sparse-scan strategy that can more effectively promote dendritic cell maturation.
(J Transl Med, 2010-01-27)BACKGROUND: The conventional treatment protocol in high-intensity focused ultrasound (HIFU) therapy utilizes a dense-scan strategy to produce closely packed thermal lesions aiming at eradicating as much tumor mass as possible. ... -
Characterization of CD4 and CD8 T cell responses in MuSK myasthenia gravis.
(J Autoimmun, 2014-08)Muscle specific tyrosine kinase myasthenia gravis (MuSK MG) is a form of autoimmune MG that predominantly affects women and has unique clinical features, including prominent bulbar weakness, muscle atrophy, and excellent ... -
Comparison of interlaboratory variation in absolute T-cell counts by single-platform and optimized dual-platform methods.
(Cytometry B Clin Cytom, 2010-05)BACKGROUND: Previous studies have reported that the adoption of a single-platform flow cytometry cell counting method resulted in lower interlaboratory variation in absolute T cell counts as compared to predicate dual-platform ... -
Computational Methods for Comparative Analysis of Rare Cell Subsets in Flow Cytometry
(2013)Automated analysis techniques for flow cytometry data can address many of the limitations of manual analysis by providing an objective approach for the identification of cellular subsets. While automated analysis has the ... -
Cryptococcal cell morphology affects host cell interactions and pathogenicity.
(PLoS Pathog, 2010-06-17)Cryptococcus neoformans is a common life-threatening human fungal pathogen. The size of cryptococcal cells is typically 5 to 10 microm. Cell enlargement was observed in vivo, producing cells up to 100 microm. These morphological ... -
Detection of amino-terminal extracellular domain of somatostatin receptor 2 by specific monoclonal antibodies and quantification of receptor density in medulloblastoma.
(Hybridoma (Larchmt), 2009-12)Somatostatin receptor 2 (SSTR2) is expressed by most medulloblastomas (MEDs). We isolated monoclonal antibodies (MAbs) to the 12-mer (33)QTEPYYDLTSNA(44), which resides in the extracellular domain of the SSTR2 amino terminus, ... -
Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling.
(PloS one, 2019-01)Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte ... -
Flow cytometric methods for prenatal and neonatal diagnosis.
(J Immunol Methods, 2011-01-05)Flow cytometry offers a promising alternative to the current methods of amniocentesis or chorionic villus sampling (CVS) for fetal cell sorting for prenatal diagnosis. While flow cytometric methods have been greatly improved ... -
FlowKit: A Python Toolkit for Integrated Manual and Automated Cytometry Analysis Workflows.
(Frontiers in immunology, 2021-01)An important challenge for primary or secondary analysis of cytometry data is how to facilitate productive collaboration between domain and quantitative experts. Domain experts in cytometry laboratories and core facilities ... -
In vivo Modeling Implicates APOL1 in Nephropathy: Evidence for Dominant Negative Effects and Epistasis under Anemic Stress.
(PLoS Genet, 2015-07)African Americans have a disproportionate risk for developing nephropathy. This disparity has been attributed to coding variants (G1 and G2) in apolipoprotein L1 (APOL1); however, there is little functional evidence supporting ... -
Inflammation triggers emergency granulopoiesis through a density-dependent feedback mechanism.
(PLoS One, 2011)Normally, neutrophil pools are maintained by homeostatic mechanisms that require the transcription factor C/EBPα. Inflammation, however, induces neutrophilia through a distinct pathway of "emergency" granulopoiesis that ... -
Integrin-mediated interactions with extracellular matrix proteins for nucleus pulposus cells of the human intervertebral disc.
(J Orthop Res, 2013-10)The extracellular matrix (ECM) of the human intervertebral disc is rich in molecules that interact with cells through integrin-mediated attachments. Porcine nucleus pulposus (NP) cells have been shown to interact with laminin ... -
Interleukin-15 receptor blockade in non-human primate kidney transplantation.
(Transplantation, 2010-04-27)BACKGROUND: Interleukin (IL)-15 is a chemotactic factor to T cells. It induces proliferation and promotes survival of activated T cells. IL-15 receptor blockade in mouse cardiac and islet allotransplant models has led to ...
