Now showing items 1-12 of 12

    • Cell-cycle control of cell polarity in yeast. 

      Moran, Kyle D; Kang, Hui; Araujo, Ana V; Zyla, Trevin R; Saito, Koji; Tsygankov, Denis; Lew, Daniel J (The Journal of cell biology, 2019-01)
      In many cells, morphogenetic events are coordinated with the cell cycle by cyclin-dependent kinases (CDKs). For example, many mammalian cells display extended morphologies during interphase but round up into more spherical ...
    • Chromatin: bind at your own RSC. 

      Buchler, Nicolas E; Bai, Lu (Curr Biol, 2011-03-22)
      Recent work has identified a novel RSC-nucleosome complex that both strongly phases flanking nucleosomes and presents regulatory sites for ready access. These results challenge several widely held views.
    • Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast. 

      Rienzo, Alessandro; Poveda-Huertes, Daniel; Aydin, Selcan; Buchler, Nicolas E; Pascual-Ahuir, Amparo; Proft, Markus (Mol Cell Biol, 2015-11)
      Cells respond to environmental stimuli by fine-tuned regulation of gene expression. Here we investigated the dose-dependent modulation of gene expression at high temporal resolution in response to nutrient and stress signals ...
    • Ferrochelatase is a conserved downstream target of the blue light-sensing White collar complex in fungi. 

      Idnurm, Alexander; Heitman, Joseph (Microbiology, 2010-08)
      Light is a universal signal perceived by organisms, including fungi, in which light regulates common and unique biological processes depending on the species. Previous research has established that conserved proteins, originally ...
    • How cells determine the number of polarity sites. 

      Chiou, Jian-Geng; Moran, Kyle D; Lew, Daniel J (eLife, 2021-04-26)
      The diversity of cell morphologies arises, in part, through regulation of cell polarity by Rho-family GTPases. A poorly understood but fundamental question concerns the regulatory mechanisms by which different cells generate ...
    • Interaction of Cryptococcus neoformans Rim101 and protein kinase A regulates capsule. 

      O'Meara, Teresa R; Norton, Diana; Price, Michael S; Hay, Christie; Clements, Meredith F; Nichols, Connie B; Alspaugh, J Andrew (PLoS Pathog, 2010-02-19)
      Cryptococcus neoformans is a prevalent human fungal pathogen that must survive within various tissues in order to establish a human infection. We have identified the C. neoformans Rim101 transcription factor, a highly conserved ...
    • Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy. 

      Mazo-Vargas, Anyimilehidi; Park, Heungwon; Aydin, Mert; Buchler, Nicolas E (Mol Biol Cell, 2014-11-05)
      Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence ...
    • Ratiometric GPCR signaling enables directional sensing in yeast. 

      Henderson, Nicholas T; Pablo, Michael; Ghose, Debraj; Clark-Cotton, Manuella R; Zyla, Trevin R; Nolen, James; Elston, Timothy C; ... (8 authors) (PLoS biology, 2019-10-17)
      Accurate detection of extracellular chemical gradients is essential for many cellular behaviors. Gradient sensing is challenging for small cells, which can experience little difference in ligand concentrations on the up-gradient ...
    • Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis. 

      Chi, Richard J; Torres, Onaidy T; Segarra, Verónica A; Lansley, Tanya; Chang, Ji Suk; Newpher, Thomas M; Lemmon, Sandra K (J Cell Sci, 2012-10-15)
      Phosphorylation regulates assembly and disassembly of proteins during endocytosis. In yeast, Prk1 and Ark1 phosphorylate factors after vesicle internalization leading to coat disassembly. Scd5, a protein phosphatase-1 ...
    • The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe. 

      Ni, Ting; Tu, Kang; Wang, Zhong; Song, Shen; Wu, Han; Xie, Bin; Scott, Kristin C; ... (10 authors) (PLoS One, 2010-12-20)
      A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, ...
    • Uncoupling of genomic and epigenetic signals in the maintenance and inheritance of heterochromatin domains in fission yeast. 

      Wheeler, Bayly S; Ruderman, Brandon T; Willard, Huntington F; Scott, Kristin C (Genetics, 2012-02)
      Many essential aspects of genome function, including gene expression and chromosome segregation, are mediated throughout development and differentiation by changes in the chromatin state. Along with genomic signals encoded ...
    • Unveiling Protein Kinase A Targets in Cryptococcus neoformans Capsule Formation. 

      Alspaugh, J Andrew (MBio, 2016-02-09)
      The protein kinase A (PKA) signal transduction pathway has been associated with pathogenesis in many fungal species. Geddes and colleagues [mBio 7(1):e01862-15, 2016, doi:10.1128/mBio.01862-15] used quantitative proteomics ...