Browsing by Subject "Genes, Reporter"
Now showing items 1-15 of 15
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A complex intronic enhancer regulates expression of the CFTR gene by direct interaction with the promoter.
(J Cell Mol Med, 2009-04)Genes can maintain spatiotemporal expression patterns by long-range interactions between cis-acting elements. The cystic fibrosis transmembrane conductance regulator gene (CFTR) is expressed primarily in epithelial cells. ... -
A selective inhibitor of eIF2alpha dephosphorylation protects cells from ER stress.
(Science (New York, N.Y.), 2005-02)Most protein phosphatases have little intrinsic substrate specificity, making selective pharmacological inhibition of specific dephosphorylation reactions a challenging problem. In a screen for small molecules that protect ... -
Allo-Specific Humoral Responses: New Methods for Screening Donor-Specific Antibody and Characterization of HLA-Specific Memory B Cells.
(Frontiers in immunology, 2021-01)Antibody-mediated allograft rejection (AMR) causes more kidney transplant failure than any other single cause. AMR is mediated by antibodies recognizing antigens expressed by the graft, and antibodies generated against major ... -
ARFGAP1 promotes the formation of COPI vesicles, suggesting function as a component of the coat.
(J Cell Biol, 2002-10-14)The role of GTPase-activating protein (GAP) that deactivates ADP-ribosylation factor 1 (ARF1) during the formation of coat protein I (COPI) vesicles has been unclear. GAP is originally thought to antagonize vesicle formation ... -
Bioengineered human myobundles mimic clinical responses of skeletal muscle to drugs.
(Elife, 2015-01-09)Existing in vitro models of human skeletal muscle cannot recapitulate the organization and function of native muscle, limiting their use in physiological and pharmacological studies. Here, we demonstrate engineering ... -
Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation.
(J Cell Sci, 2010-07-01)Wg/Wnt signals specify cell fates in both invertebrate and vertebrate embryos and maintain stem-cell populations in many adult tissues. Deregulation of the Wnt pathway can transform cells to a proliferative fate, leading ... -
Imaging-Based Reporter Systems to Define CVB-Induced Membrane Remodeling in Living Cells.
(Viruses, 2020-09-25)Enteroviruses manipulate host membranes to form replication organelles, which concentrate viral and host factors to allow for efficient replication. However, this process has not been well-studied in living cells throughout ... -
Intrastromal Gene Therapy Prevents and Reverses Advanced Corneal Clouding in a Canine Model of Mucopolysaccharidosis I.
(Molecular therapy : the journal of the American Society of Gene Therapy, 2020-06)Mucopolysaccharidosis type I (MPS I) is an autosomal recessive lysosomal storage disease characterized by severe phenotypes, including corneal clouding. MPS I is caused by mutations in alpha-l-iduronidase (IDUA), a ubiquitous ... -
Localization of DIR1 at the tissue, cellular and subcellular levels during Systemic Acquired Resistance in Arabidopsis using DIR1:GUS and DIR1:EGFP reporters.
(BMC plant biology, 2011-01)BACKGROUND: Systemic Acquired Resistance (SAR) is an induced resistance response to pathogens, characterized by the translocation of a long-distance signal from induced leaves to distant tissues to prime them for increased ... -
Pairing of competitive and topologically distinct regulatory modules enhances patterned gene expression.
(Mol Syst Biol, 2008)Biological networks are inherently modular, yet little is known about how modules are assembled to enable coordinated and complex functions. We used RNAi and time series, whole-genome microarray analyses to systematically ... -
Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung.
(Nature communications, 2015-04-13)The plasticity of differentiated cells in adult tissues undergoing repair is an area of intense research. Pulmonary alveolar type II cells produce surfactant and function as progenitors in the adult, demonstrating ... -
Ratiometric GPCR signaling enables directional sensing in yeast.
(PLoS biology, 2019-10-17)Accurate detection of extracellular chemical gradients is essential for many cellular behaviors. Gradient sensing is challenging for small cells, which can experience little difference in ligand concentrations on the up-gradient ... -
RNA-Seq and ChIP-Seq reveal SQSTM1/p62 as a key mediator of JunB suppression of NF-κB-dependent inflammation.
(J Invest Dermatol, 2015-04)Mice with epidermal deletion of JunB transcription factor displayed a psoriasis-like inflammation. The relevance of these findings to humans and the mechanisms mediating JunB function are not fully understood. Here we demonstrate ... -
Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling.
(J Vis Exp, 2015-09-14)Transsynaptic tracing has become a powerful tool used to analyze central efferents that regulate peripheral targets through multi-synaptic circuits. This approach has been most extensively used in the brain by utilizing ... -
Unmasking Proteolytic Activity for Adult Visual Cortex Plasticity by the Removal of Lynx1.
(The Journal of neuroscience : the official journal of the Society for Neuroscience, 2015-09)<h4>Unlabelled</h4>Experience-dependent cortical plasticity declines with age. At the molecular level, experience-dependent proteolytic activity of tissue plasminogen activator (tPA) becomes restricted in the adult brain ...
