Browsing by Subject "Hela Cells"
Now showing items 1-12 of 12
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A genome-wide RNAi screen reveals multiple regulators of caspase activation.
(The Journal of cell biology, 2007-11-12)Apoptosis is an evolutionally conserved cellular suicide mechanism that can be activated in response to a variety of stressful stimuli. Increasing evidence suggests that apoptotic regulation relies on specialized cell death ... -
Association with endoplasmic reticulum promotes proteasomal degradation of GADD34 protein.
(The Journal of biological chemistry, 2011-06)Stress-induced endogenous and ectopically expressed GADD34 proteins were present both in the cytoplasm and in membranes, with their membrane association showing similar biochemical properties. Deletion of N-terminal sequences ... -
BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation.
(Cell reports, 2020-09)Effective spatio-temporal control of transcription and replication during S-phase is paramount to maintaining genomic integrity and cell survival. Dysregulation of these systems can lead to conflicts between the transcription ... -
Chronic oxidative stress promotes GADD34-mediated phosphorylation of the TAR DNA-binding protein TDP-43, a modification linked to neurodegeneration.
(The Journal of biological chemistry, 2018-01)Oxidative and endoplasmic reticulum (ER) stresses are hallmarks of the pathophysiology of ALS and other neurodegenerative diseases. In these stresses, different kinases phosphorylate eukaryotic initiation factor eIF2α, enabling ... -
Circulating tumor cells exit circulation while maintaining multicellularity, augmenting metastatic potential.
(Journal of cell science, 2019-09)Metastasis accounts for the majority of all cancer deaths, yet the process remains poorly understood. A pivotal step in the metastasis process is the exiting of tumor cells from the circulation, a process known as extravasation. ... -
DEK is required for homologous recombination repair of DNA breaks.
(Scientific reports, 2017-03-20)DEK is a highly conserved chromatin-bound protein whose upregulation across cancer types correlates with genotoxic therapy resistance. Loss of DEK induces genome instability and sensitizes cells to DNA double strand breaks ... -
Esterified Trehalose Analogues Protect Mammalian Cells from Heat Shock.
(Chembiochem : a European journal of chemical biology, 2017-09)Trehalose is a disaccharide produced by many organisms to better enable them to survive environmental stresses, including heat, cold, desiccation, and reactive oxygen species. Mammalian cells do not naturally biosynthesize ... -
Inhibition of the futalosine pathway for menaquinone biosynthesis suppresses Chlamydia trachomatis infection.
(FEBS letters, 2021-12)Chlamydia trachomatis, an obligate intracellular bacterium with limited metabolic capabilities, possesses the futalosine pathway for menaquinone biosynthesis. Futalosine pathway enzymes have promise as narrow-spectrum antibiotic ... -
Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners.
(Proceedings of the National Academy of Sciences of the United States of America, 2012-03-12)O-linked β-N-acetylglucosamine (O-GlcNAc) is a reversible posttranslational modification found on hundreds of nuclear and cytoplasmic proteins in higher eukaryotes. Despite its ubiquity and essentiality in mammals, functional ... -
Phosphorylation at tyrosine 262 promotes GADD34 protein turnover.
(The Journal of biological chemistry, 2013-11)In mammalian cells, metabolic and environmental stress increases the phosphorylation of the eukaryotic translational initiation factor, eIF2α, and attenuates global protein synthesis. Subsequent transcriptional activation ... -
The APC/C E3 Ligase Complex Activator FZR1 Restricts BRAF Oncogenic Function.
(Cancer discovery, 2017-04)BRAF drives tumorigenesis by coordinating the activation of the RAS/RAF/MEK/ERK oncogenic signaling cascade. However, upstream pathways governing BRAF kinase activity and protein stability remain undefined. Here, we report ... -
Tumor cell-derived exosomes home to their cells of origin and can be used as Trojan horses to deliver cancer drugs.
(Theranostics, 2020-01)Cancer is the second leading cause of death worldwide and patients are in urgent need of therapies that can effectively target cancer with minimal off-target side effects. Exosomes are extracellular nano-shuttles that facilitate ...
