Browsing by Subject "In Vitro Techniques"
Now showing items 1-16 of 16
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An approach to the study of G-protein-coupled receptor kinases: an in vitro-purified membrane assay reveals differential receptor specificity and regulation by G beta gamma subunits.
(Proc Natl Acad Sci U S A, 1994-04-26)Phosphorylation of GTP-binding-regulatory (G)-protein-coupled receptors by specific G-protein-coupled receptor kinases (GRKs) is a major mechanism responsible for agonist-mediated desensitization of signal transduction processes. ... -
Antagonists of the system L neutral amino acid transporter (LAT) promote endothelial adhesivity of human red blood cells.
(Thrombosis and haemostasis, 2017-06)The system L neutral amino acid transporter (LAT; LAT1, LAT2, LAT3, or LAT4) has multiple functions in human biology, including the cellular import of S-nitrosothiols (SNOs), biologically active derivatives of nitric oxide ... -
Cyclosporine A inhibits Ca2+-dependent stimulation of the Na+/H+ antiport in human T cells.
(J Cell Biol, 1986-08)The cyclic undecapeptide cyclosporine A (CsA) is a potent immunosuppressive agent that inhibits the initial activation of T lymphocytes. This agent appears to be most effective in blocking the action of mitogens such as ... -
Direct evidence that Gi-coupled receptor stimulation of mitogen-activated protein kinase is mediated by G beta gamma activation of p21ras.
(Proc Natl Acad Sci U S A, 1994-12-20)Stimulation of Gi-coupled receptors leads to the activation of mitogen-activated protein kinases (MAP kinases). In several cell types, this appears to be dependent on the activation of p21ras (Ras). Which G-protein subunit(s) ... -
Functional desensitization of the isolated beta-adrenergic receptor by the beta-adrenergic receptor kinase: potential role of an analog of the retinal protein arrestin (48-kDa protein).
(Proc Natl Acad Sci U S A, 1987-12)The beta-adrenergic receptor kinase is an enzyme, possibly analogous to rhodopsin kinase, that multiply phosphorylates the beta-adrenergic receptor only when it is occupied by stimulatory agonists. Since this kinase may ... -
Functionally active targeting domain of the beta-adrenergic receptor kinase: an inhibitor of G beta gamma-mediated stimulation of type II adenylyl cyclase.
(Proc Natl Acad Sci U S A, 1994-04-26)The beta-adrenergic receptor kinase (beta ARK) phosphorylates its membrane-associated receptor substrates, such as the beta-adrenergic receptor, triggering events leading to receptor desensitization. beta ARK activity is ... -
High-sensitivity rod photoreceptor input to the blue-yellow color opponent pathway in macaque retina.
(Nat Neurosci, 2009-09)Small bistratified cells (SBCs) in the primate retina carry a major blue-yellow opponent signal to the brain. We found that SBCs also carry signals from rod photoreceptors, with the same sign as S cone input. SBCs exhibited ... -
In vitro fluid dynamics of the Ahmed glaucoma valve modified with expanded polytetrafluoroethylene.
(Curr Eye Res, 2011-02)PURPOSE: Long-term intraocular pressure reduction by glaucoma drainage devices (GDDs) is often limited by the fibrotic capsule that forms around them. Prior work demonstrates that modifying a GDD with a porous membrane promotes ... -
Isolation, purification and in vitro differentiation of cytotrophoblast cells from human term placenta.
(Reproductive biology and endocrinology : RB&E, 2015-07)<h4>Background</h4>The syncytialization of cytotrophoblast cells to syncytiotrophoblast is central to human placental transport and hormone production. Many techniques for in vitro study of this process have been proposed ... -
Multivalent benzoboroxole functionalized polymers as gp120 glycan targeted microbicide entry inhibitors.
(Mol Pharm, 2010-02-01)Microbicides are women-controlled prophylactics for sexually transmitted infections. The most important class of microbicides target HIV-1 and contain antiviral agents formulated for topical vaginal delivery. Identification ... -
Pollutant particles produce vasoconstriction and enhance MAPK signaling via angiotensin type I receptor.
(Environmental health perspectives, 2005-08)Exposure to particulate matter (PM) is associated with acute cardiovascular mortality and morbidity, but the mechanisms are not entirely clear. In this study, we hypothesized that PM may activate the angiotensin type 1 receptor ... -
PPAR-δ is repressed in Huntington's disease, is required for normal neuronal function and can be targeted therapeutically.
(Nat Med, 2016-01)Huntington's disease (HD) is a progressive neurodegenerative disorder caused by a CAG trinucleotide repeat expansion in the huntingtin (HTT) gene, which encodes a polyglutamine tract in the HTT protein. We found that peroxisome ... -
Retinal representation of the elementary visual signal.
(Neuron, 2014-01)The propagation of visual signals from individual cone photoreceptors through parallel neural circuits was examined in the primate retina. Targeted stimulation of individual cones was combined with simultaneous recording ... -
SHP-1 as a critical regulator of Mycoplasma pneumoniae-induced inflammation in human asthmatic airway epithelial cells.
(Journal of immunology (Baltimore, Md. : 1950), 2012-04)Asthma is a chronic inflammatory disease in which airway epithelial cells are the first line of defense against exposure of the airway to infectious agents. Src homology protein (SHP)-1, a protein tyrosine phosphatase, is ... -
Tissue type-specific expression of the dsRNA-binding protein 76 and genome-wide elucidation of its target mRNAs.
(PLoS One, 2010-07-23)BACKGROUND: RNA-binding proteins accompany all steps in the life of mRNAs and provide dynamic gene regulatory functions for rapid adjustment to changing extra- or intracellular conditions. The association of RNA-binding ...
