Browsing by Subject "Mutagenesis, Site-Directed"
Now showing items 1-5 of 5
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Evidence for an electrostatic mechanism of force generation by the bacteriophage T4 DNA packaging motor.
(Nat Commun, 2014-06-17)How viral packaging motors generate enormous forces to translocate DNA into viral capsids remains unknown. Recent structural studies of the bacteriophage T4 packaging motor have led to a proposed mechanism wherein the gp17 ... -
Facilitative glucose transporter Glut1 is actively excluded from rod outer segments.
(J Cell Sci, 2010-11-01)Photoreceptors are among the most metabolically active cells in the body, relying on both oxidative phosphorylation and glycolysis to satisfy their high energy needs. Local glycolysis is thought to be particularly crucial ... -
Long range dynamic effects of point-mutations trap a response regulator in an active conformation.
(FEBS letters, 2010-10)When a point-mutation in a protein elicits a functional change, it is most common to assign this change to local structural perturbations. Here we show that point-mutations, distant from an essential highly dynamic kinase ... -
Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy.
(Proc Natl Acad Sci U S A, 1994-10-11)Transgenic mice were generated by using the alpha-myosin heavy chain promoter coupled to the coding sequence of a constitutively active mutant alpha 1B-adrenergic receptor (AR). These transgenic animals demonstrated ... -
R5 clade C SHIV strains with tier 1 or 2 neutralization sensitivity: tools to dissect env evolution and to develop AIDS vaccines in primate models.
(PLoS One, 2010-07-21)BACKGROUND: HIV-1 clade C (HIV-C) predominates worldwide, and anti-HIV-C vaccines are urgently needed. Neutralizing antibody (nAb) responses are considered important but have proved difficult to elicit. Although some current ...
