Browsing by Subject "Observer Variation"
Now showing items 1-20 of 23
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An automated method for comparing motion artifacts in cine four-dimensional computed tomography images.
(Journal of applied clinical medical physics, 2012-11-08)The aim of this study is to develop an automated method to objectively compare motion artifacts in two four-dimensional computed tomography (4D CT) image sets, and identify the one that would appear to human observers with ... -
Automatic segmentation of seven retinal layers in SDOCT images congruent with expert manual segmentation.
(Opt Express, 2010-08-30)Segmentation of anatomical and pathological structures in ophthalmic images is crucial for the diagnosis and study of ocular diseases. However, manual segmentation is often a time-consuming and subjective process. This paper ... -
Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays.
(Journal of Immunological Methods, 2014-07)Luminex bead array assays are widely used for rapid biomarker quantification due to the ability to measure up to 100 unique analytes in a single well of a 96-well plate. There has been, however, no comprehensive analysis ... -
Development of a contemporary globally diverse HIV viral panel by the EQAPOL program.
(J Immunol Methods, 2014-07)The significant diversity among HIV-1 variants poses serious challenges for vaccine development and for developing sensitive assays for screening, surveillance, diagnosis, and clinical management. Recognizing a need to develop ... -
Establishment and maintenance of a PBMC repository for functional cellular studies in support of clinical vaccine trials.
(J Immunol Methods, 2014-07)A large repository of cryopreserved peripheral blood mononuclear cells (PBMCs) samples was created to provide laboratories testing the specimens from human immunodeficiency virus-1 (HIV-1) vaccine clinical trials the material ... -
Impact of beta-blockade premedication on image quality of ECG-gated thoracic aorta CT angiography.
(Acta radiologica (Stockholm, Sweden : 1987), 2014-12)BACKGROUND: Thoracic aortic aneurysm is one of the most common aorta pathologies worldwide, which is commonly evaluated by computed tomography angiography (CTA). One of the routine methods to improve the image quality of ... -
Implementing a Continuous Quality Improvement Program in a High-Volume Clinical Echocardiography Laboratory: Improving Care for Patients With Aortic Stenosis.
(Circ Cardiovasc Imaging, 2016-03)BACKGROUND: The management of aortic stenosis rests on accurate echocardiographic diagnosis. Hence, it was chosen as a test case to examine the utility of continuous quality improvement (CQI) approaches to increase ... -
Intraobserver variability in fibroid size measurements: estimated effects on assessing fibroid growth.
(Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine, 2014-07)To evaluate intraobserver variability of fibroid sonographic measurements and apply this factor to fibroid growth assessment.Study participants were African American women aged 23 to 34 years who had never had a diagnosis ... -
Introduction to a Special Issue of the Journal of Immunological Methods: Building global resource programs to support HIV/AIDS clinical trial studies.
(J Immunol Methods, 2014-07)This Special Issue of the Journal of Immunological Methods includes 16 manuscripts describing quality assurance activities related to virologic and immunologic monitoring of six global laboratory resource programs that support ... -
Leukopak PBMC sample processing for preparing quality control material to support proficiency testing programs.
(Journal of Immunological Methods, 2014-07)External proficiency testing programs designed to evaluate the performance of end-point laboratories involved in vaccine and therapeutic clinical trials form an important part of clinical trial quality assurance. Good clinical ... -
Objective assessment of vaginal surgical skills.
(Am J Obstet Gynecol, 2010-07)OBJECTIVE: To develop and validate an instrument to assess surgical skills during vaginal surgery. STUDY DESIGN: Trainees from 2 institutions were directly assessed in the operating room by supervising surgeons while performing ... -
Optimization and validation of a neutralizing antibody assay for HIV-1 in A3R5 cells.
(Journal of immunological methods, 2014-07)A3R5 is a human CD4(+) lymphoblastoid cell line that was engineered to express CCR5 and is useful for the detection of weak neutralizing antibody responses against tier 2 strains of HIV-1. Here we describe the optimization ... -
Optimization and validation of a neutralizing antibody assay for HIV-1 in A3R5 cells.
(J Immunol Methods, 2014-07)A3R5 is a human CD4(+) lymphoblastoid cell line that was engineered to express CCR5 and is useful for the detection of weak neutralizing antibody responses against tier 2 strains of HIV-1. Here we describe the optimization ... -
Right coronary wall CMR in the older asymptomatic advance cohort: positive remodeling and associations with type 2 diabetes and coronary calcium.
(J Cardiovasc Magn Reson, 2010-12-30)BACKGROUND: Coronary wall cardiovascular magnetic resonance (CMR) is a promising noninvasive approach to assess subclinical atherosclerosis, but data are limited in subjects over 60 years old, who are at increased risk. ... -
Setting objective thresholds for rare event detection in flow cytometry.
(J Immunol Methods, 2014-07)The accurate identification of rare antigen-specific cytokine positive cells from peripheral blood mononuclear cells (PBMC) after antigenic stimulation in an intracellular staining (ICS) flow cytometry assay is challenging, ... -
Standardization and validation of a novel and simple method to assess lumbar dural sac size.
(Clinical radiology, 2015-02)To develop and validate a simple, reproducible method to assess dural sac size using standard imaging technology.This study was institutional review board-approved. Two readers, blinded to the diagnoses, measured ... -
Statistical methods for the assessment of EQAPOL proficiency testing: ELISpot, Luminex, and Flow Cytometry.
(Journal of Immunological Methods, 2014-07)In September 2011 Duke University was awarded a contract to develop the National Institutes of Health/National Institute of Allergy and Infectious Diseases (NIH/NIAID) External Quality Assurance Program Oversight Laboratory ... -
Testing parallel laser image scaling for remotely measuring body dimensions on mantled howling monkeys (Alouatta palliata).
(Am J Primatol, 2015-08)Body size is a fundamental variable for many studies in primate biology. However, obtaining body dimensions of wild primates through live capture is difficult and costly, so developing an alternative inexpensive and non-invasive ... -
The Center for HIV/AIDS Vaccine Immunology (CHAVI) multi-site quality assurance program for cryopreserved human peripheral blood mononuclear cells.
(J Immunol Methods, 2014-07)The Center for HIV/AIDS Vaccine Immunology (CHAVI) consortium was established to determine the host and virus factors associated with HIV transmission, infection and containment of virus replication, with the goal of advancing ... -
The External Quality Assurance Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay.
(Journal of Immunological Methods, 2014-07)The interferon-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay has been developed and used as an end-point assay in clinical trials for infectious diseases and cancer to detect the magnitude of antigen-specific immune ...