Browsing by Subject "Plasmids"
Now showing items 1-9 of 9
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A functional analysis of the spacer of V(D)J recombination signal sequences.
(PLoS Biol, 2003-10)During lymphocyte development, V(D)J recombination assembles antigen receptor genes from component V, D, and J gene segments. These gene segments are flanked by a recombination signal sequence (RSS), which serves as the ... -
G-protein-coupled receptor genes as protooncogenes: constitutively activating mutation of the alpha 1B-adrenergic receptor enhances mitogenesis and tumorigenicity.
(Proc Natl Acad Sci U S A, 1991-12-15)The alpha 1B-adrenergic receptor (alpha 1B-ADR) is a member of the G-protein-coupled family of transmembrane receptors. When transfected into Rat-1 and NIH 3T3 fibroblasts, this receptor induces focus formation ... -
Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division.
(Proceedings of the National Academy of Sciences of the United States of America, 2013-11-11)Bacteriophages take over host resources primarily via the activity of proteins expressed early in infection. One of these proteins, produced by the Escherichia coli phage T7, is gene product (Gp) 0.4. Here, we show that ... -
Imaging-Based Reporter Systems to Define CVB-Induced Membrane Remodeling in Living Cells.
(Viruses, 2020-09-25)Enteroviruses manipulate host membranes to form replication organelles, which concentrate viral and host factors to allow for efficient replication. However, this process has not been well-studied in living cells throughout ... -
Membrane binding of plasmid DNA and endocytic pathways are involved in electrotransfection of mammalian cells.
(PLoS One, 2011)Electric field mediated gene delivery or electrotransfection is a widely used method in various studies ranging from basic cell biology research to clinical gene therapy. Yet, mechanisms of electrotransfection are still ... -
Recursive directional ligation by plasmid reconstruction allows rapid and seamless cloning of oligomeric genes.
(Biomacromolecules, 2010-04-12)This paper reports a new strategy, recursive directional ligation by plasmid reconstruction (PRe-RDL), to rapidly clone highly repetitive polypeptides of any sequence and specified length over a large range of molecular ... -
Regions of the alpha 1-adrenergic receptor involved in coupling to phosphatidylinositol hydrolysis and enhanced sensitivity of biological function.
(Proc Natl Acad Sci U S A, 1990-04)Regions of the hamster alpha 1-adrenergic receptor (alpha 1 AR) that are important in GTP-binding protein (G protein)-mediated activation of phospholipase C were determined by studying the biological functions of mutant ... -
Separating DNA with different topologies by atomic force microscopy in comparison with gel electrophoresis.
(J Phys Chem B, 2010-09-23)Atomic force microscopy, which is normally used for DNA imaging to gain qualitative results, can also be used for quantitative DNA research, at a single-molecular level. Here, we evaluate the performance of AFM ... -
Surfactant protein A is defective in abrogating inflammation in asthma.
(American journal of physiology. Lung cellular and molecular physiology, 2011-10)Surfactant protein A (SP-A) regulates a variety of immune cell functions. We determined the ability of SP-A derived from normal and asthmatic subjects to modulate the inflammatory response elicited by Mycoplasma pneumoniae, ...
