Browsing by Subject "Polymerase Chain Reaction"
Now showing items 1-20 of 32
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A novel mutation of the ACADM gene (c.145C>G) associated with the common c.985A>G mutation on the other ACADM allele causes mild MCAD deficiency: a case report.
(Orphanet J Rare Dis, 2010-10-05)A female patient, with normal familial history, developed at the age of 30 months an episode of diarrhoea, vomiting and lethargy which resolved spontaneously. At the age of 3 years, the patient re-iterated vomiting, was ... -
A recessive variant of XRCC4 predisposes to non- BRCA1/2 breast cancer in chinese women and impairs the DNA damage response via dysregulated nuclear localization.
(Oncotarget, 2014-12)XRCC4 plays a crucial role in the non-homologous end joining pathway that maintains genome stability. In this two-stage case-control study with 1,764 non-BRCA1/2 breast cancer patients and 1,623 cancer-free controls, we ... -
CCDC62/ERAP75 functions as a coactivator to enhance estrogen receptor beta-mediated transactivation and target gene expression in prostate cancer cells.
(Carcinogenesis, 2009-05)Human prostate cancer (PCa) and prostate epithelial cells predominantly express estrogen receptor (ER) beta, but not ERalpha. ERbeta might utilize various ER coregulators to mediate the E2-signaling pathway in PCa. Here, ... -
Cryptococcus gattii VGIII isolates causing infections in HIV/AIDS patients in Southern California: identification of the local environmental source as arboreal.
(PLoS Pathog, 2014-08)Ongoing Cryptococcus gattii outbreaks in the Western United States and Canada illustrate the impact of environmental reservoirs and both clonal and recombining propagation in driving emergence and expansion of microbial ... -
Cyanobacterial ribosomal RNA genes with multiple, endonuclease-encoding group I introns.
(BMC evolutionary biology, 2007-09-08)Group I introns are one of the four major classes of introns as defined by their distinct splicing mechanisms. Because they catalyze their own removal from precursor transcripts, group I introns are referred to as autocatalytic ... -
Delimiting species without nuclear monophyly in Madagascar's mouse lemurs.
(PLoS One, 2010-03-31)BACKGROUND: Speciation begins when populations become genetically separated through a substantial reduction in gene flow, and it is at this point that a genetically cohesive set of populations attain the sole property of ... -
Detection of Bartonella species in the blood of veterinarians and veterinary technicians: a newly recognized occupational hazard?
(Vector Borne Zoonotic Dis, 2014-08)BACKGROUND: Bartonella species are important emerging pathogens in human and veterinary medicine. In the context of their daily activities, veterinary professionals have frequent animal contact and arthropod exposures. Detection ... -
Dual infection with Ehrlichia chaffeensis and a spotted fever group rickettsia: a case report.
(Emerging infectious diseases, 1998-04)Well-documented cases of simultaneous human infection with more than one tick-borne pathogen are rare. To our knowledge only two dual infections have been reported: simultaneous human infection with the agent of ... -
Emergence and pathogenicity of highly virulent Cryptococcus gattii genotypes in the northwest United States.
(PLoS Pathog, 2010-04-22)Cryptococcus gattii causes life-threatening disease in otherwise healthy hosts and to a lesser extent in immunocompromised hosts. The highest incidence for this disease is on Vancouver Island, Canada, where an outbreak is ... -
Essential role of beta-adrenergic receptor kinase 1 in cardiac development and function.
(Proc Natl Acad Sci U S A, 1996-11-12)The beta-adrenergic receptor kinase 1 (beta ARK1) is a member of the G protein-coupled receptor kinase (GRK) family that mediates the agonist-dependent phosphorylation and desensitization of G protein-coupled receptors. ... -
Evidence that SOX2 overexpression is oncogenic in the lung.
(PLoS One, 2010-06-10)BACKGROUND: SOX2 (Sry-box 2) is required to maintain a variety of stem cells, is overexpressed in some solid tumors, and is expressed in epithelial cells of the lung. METHODOLOGY/PRINCIPAL FINDINGS: We show that SOX2 is ... -
Expansion of the alpha 2-adrenergic receptor family: cloning and characterization of a human alpha 2-adrenergic receptor subtype, the gene for which is located on chromosome 2.
(Proc Natl Acad Sci U S A, 1990-07)Pharmacologic, biochemical, and genetic analyses have demonstrated the existence of multiple alpha 2-adrenergic receptor (alpha 2AR) subtypes. We have cloned a human alpha 2AR by using the polymerase chain reaction with ... -
From offshore to onshore: multiple origins of shallow-water corals from deep-sea ancestors.
(PLoS One, 2008-06-18)Shallow-water tropical reefs and the deep sea represent the two most diverse marine environments. Understanding the origin and diversification of this biodiversity is a major quest in ecology and evolution. The most prominent ... -
In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. III. The kinetics of V region mutation and selection in germinal center B cells.
(J Exp Med, 1993-10-01)In the murine spleen, germinal centers are the anatomic sites for antigen-driven hypermutation and selection of immunoglobulin (Ig) genes. To detail the kinetics of Ig mutation and selection, 178 VDJ sequences from ... -
Interactions between life stress factors and carrying the APOE4 allele adversely impact self-reported health in old adults.
(The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 2011-10)Based on the multiple logistic regression analysis of data from a random sample of 1,023 old adults collected in Taiwan in 2000, we found that interactions between carrying the APOE4 allele and one of four life stress factors ... -
Invasive aspergillosis in critically ill patients: Review of definitions and diagnostic approaches.
(Mycoses, 2021-09)Invasive aspergillosis (IA) is an increasingly recognised phenomenon in critically ill patients in the intensive care unit, including in patients with severe influenza and severe coronavirus disease 2019 (COVID-19) infection. ... -
Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens.
(Diagn Microbiol Infect Dis, 2010-05)Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and serology. Polymerase chain reaction (PCR) has emerged as a sensitive method to ... -
Molecular characterization of chordoma xenografts generated from a novel primary chordoma cell source and two chordoma cell lines.
(J Neurosurg Spine, 2014-09)OBJECT: Chordoma cells can generate solid-like tumors in xenograft models that express some molecular characteristics of the parent tumor, including positivity for brachyury and cytokeratins. However, there is a dearth of ... -
Morphological and genomic characterization of Filobasidiella depauperata: a homothallic sibling species of the pathogenic cryptococcus species complex.
(PLoS One, 2010-03-10)The fungal species Cryptococcus neoformans and Cryptococcus gattii cause respiratory and neurological disease in animals and humans following inhalation of basidiospores or desiccated yeast cells from the environment. Sexual ...
