Browsing by Subject "Proteins"
Now showing items 1-20 of 36
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A robotics platform for automated batch fabrication of high density, microfluidics-based DNA microarrays, with applications to single cell, multiplex assays of secreted proteins.
(The Review of scientific instruments, 2011-09)Microfluidics flow-patterning has been utilized for the construction of chip-scale miniaturized DNA and protein barcode arrays. Such arrays have been used for specific clinical and fundamental investigations in which many ... -
A selective inhibitor of eIF2alpha dephosphorylation protects cells from ER stress.
(Science (New York, N.Y.), 2005-02)Most protein phosphatases have little intrinsic substrate specificity, making selective pharmacological inhibition of specific dephosphorylation reactions a challenging problem. In a screen for small molecules that protect ... -
Analysis of oxygen/glucose-deprivation-induced changes in SUMO3 conjugation using SILAC-based quantitative proteomics.
(Journal of proteome research, 2012-02)Transient cerebral ischemia dramatically activates small ubiquitin-like modifier (SUMO2/3) conjugation. In cells exposed to 6 h of transient oxygen/glucose deprivation (OGD), a model of ischemia, SUMOylation increases profoundly ... -
Associations between antibiotic exposure during pregnancy, birth weight and aberrant methylation at imprinted genes among offspring.
(International journal of obesity (2005), 2013-07)<h4>Objectives</h4>Low birth weight (LBW) has been associated with common adult-onset chronic diseases, including obesity, cardiovascular disease, type II diabetes and some cancers. The etiology of LBW is multi-factorial. ... -
Barnacle cement: a polymerization model based on evolutionary concepts.
(2009-11)The tenacity by which barnacles adhere has sparked a long history of scientific investigation into their adhesive mechanisms. To adhere, barnacles utilize proteinaceous cement that rapidly polymerizes and forms adhesive ... -
Bayesian inference for genomic data integration reduces misclassification rate in predicting protein-protein interactions.
(PLoS Comput Biol, 2011-07)Protein-protein interactions (PPIs) are essential to most fundamental cellular processes. There has been increasing interest in reconstructing PPIs networks. However, several critical difficulties exist in obtaining reliable ... -
beta-Arrestin1 modulates lymphoid enhancer factor transcriptional activity through interaction with phosphorylated dishevelled proteins.
(Proc Natl Acad Sci U S A, 2001-12-18)One aspect of the function of the beta-arrestins is to serve as scaffold or adapter molecules coupling G-protein coupled receptors (GPCRs) to signal transduction pathways distinct from traditional second messenger pathways. ... -
beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein.
(Proc Natl Acad Sci U S A, 1998-11-24)G protein-coupled receptor activation leads to the membrane recruitment and activation of G protein-coupled receptor kinases, which phosphorylate receptors and lead to their inactivation. We have identified a novel ... -
Competition between monomeric and dimeric crystals in schematic models for globular proteins.
(J Phys Chem B, 2014-07-17)Advances in experimental techniques and in theoretical models have improved our understanding of protein crystallization. However, they have also left open questions regarding the protein phase behavior and self-assembly ... -
Computational crystallization.
(Arch Biochem Biophys, 2016-07-15)Crystallization is a key step in macromolecular structure determination by crystallography. While a robust theoretical treatment of the process is available, due to the complexity of the system, the experimental process ... -
Crystallization of asymmetric patchy models for globular proteins in solution.
(Phys Rev E Stat Nonlin Soft Matter Phys, 2013-07)Asymmetric patchy particle models have recently been shown to describe the crystallization of small globular proteins with near-quantitative accuracy. Here, we investigate how asymmetry in patch geometry and bond energy ... -
Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation.
(Journal of visualized experiments : JoVE, 2019-01-16)Advancements in the cost and speed of next generation genetic sequencing have generated an explosion of clinical whole exome and whole genome testing. While this has led to increased identification of likely pathogenic mutations ... -
Domain-oriented edge-based alignment of protein interaction networks.
(Bioinformatics, 2009-06-15)MOTIVATION: Recent advances in high-throughput experimental techniques have yielded a large amount of data on protein-protein interactions (PPIs). Since these interactions can be organized into networks, and since separate ... -
Electrosprayed core-shell microspheres for protein delivery.
(Chem Commun (Camb), 2010-07-14)This communication describes a single-step electrospraying technique that generates core-shell microspheres (CSMs) with encapsulated protein as the core and an amphiphilic biodegradable polymer as the shell. The protein ... -
Evolutionary Divergence of Gene and Protein Expression in the Brains of Humans and Chimpanzees.
(Genome Biol Evol, 2015-07-10)Although transcriptomic profiling has become the standard approach for exploring molecular differences in the primate brain, very little is known about how the expression levels of gene transcripts relate to downstream protein ... -
G protein beta gamma subunits stimulate phosphorylation of Shc adapter protein.
(Proc Natl Acad Sci U S A, 1995-09-26)The mechanism of mitogen-activated protein (MAP) kinase activation by pertussis toxin-sensitive Gi-coupled receptors is known to involve the beta gamma subunits of heterotrimeric G proteins (G beta gamma), p21ras activation, ... -
HDAC6 and Ubp-M BUZ domains recognize specific C-terminal sequences of proteins.
(Biochemistry, 2010-12-21)The BUZ/Znf-UBP domain is a protein module found in the cytoplasmic deacetylase HDAC6, E3 ubiquitin ligase BRAP2/IMP, and a subfamily of ubiquitin-specific proteases. Although several BUZ domains have been shown to bind ... -
HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.
(Genes Dev, 2007-04-01)In response to DNA damage, p53 undergoes post-translational modifications (including acetylation) that are critical for its transcriptional activity. However, the mechanism by which p53 acetylation is regulated is still ... -
Identification of cis-suppression of human disease mutations by comparative genomics.
(Nature, 2015-08)Patterns of amino acid conservation have served as a tool for understanding protein evolution. The same principles have also found broad application in human genomics, driven by the need to interpret the pathogenic potential ... -
Intercellular Protein Transfer from Thymocytes to Thymic Epithelial Cells.
(PLoS One, 2016)Promiscuous expression of tissue restricted antigens (TRAs) in medullary thymic epithelial cells (mTECs) is crucial for negative selection of self-reactive T cells to establish central tolerance. Intercellular transfer of ...
