Now showing items 1-10 of 10

    • An interview with Professor Robert J. Lefkowitz, M.D. Interview by Vicki Glaser. 

      Lefkowitz, Robert J (Assay Drug Dev Technol, 2003-04)
      Robert J. Lefkowitz, M.D., is James B. Duke Professor of Medicine and Professor of Biochemistry at the Duke University Medical Center. He has been an Investigator of the Howard Hughes Medical Institute since 1976. Dr. Lefkowitz ...
    • beta-Arrestin1 mediates nicotinic acid-induced flushing, but not its antilipolytic effect, in mice. 

      Walters, Robert W; Shukla, Arun K; Kovacs, Jeffrey J; Violin, Jonathan D; DeWire, Scott M; Lam, Christopher M; Chen, J Ruthie; ... (10 authors) (J Clin Invest, 2009-05)
      Nicotinic acid is one of the most effective agents for both lowering triglycerides and raising HDL. However, the side effect of cutaneous flushing severely limits patient compliance. As nicotinic acid stimulates the GPCR ...
    • Direct evidence that Gi-coupled receptor stimulation of mitogen-activated protein kinase is mediated by G beta gamma activation of p21ras. 

      Koch, WJ; Hawes, BE; Allen, LF; Lefkowitz, RJ (Proc Natl Acad Sci U S A, 1994-12-20)
      Stimulation of Gi-coupled receptors leads to the activation of mitogen-activated protein kinases (MAP kinases). In several cell types, this appears to be dependent on the activation of p21ras (Ras). Which G-protein subunit(s) ...
    • Identification of select glucocorticoids as Smoothened agonists: potential utility for regenerative medicine. 

      Wang, Jiangbo; Lu, Jiuyi; Bond, Michael C; Chen, Minyong; Ren, Xiu-Rong; Lyerly, H Kim; Barak, Larry S; ... (8 authors) (Proc Natl Acad Sci U S A, 2010-05-18)
      Regenerative medicine holds the promise of replacing damaged tissues largely by stem cell activation. Hedgehog signaling through the plasma membrane receptor Smoothened (Smo) is an important process for regulating stem cell ...
    • Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases. 

      Oppermann, M; Diversé-Pierluissi, M; Drazner, MH; Dyer, SL; Freedman, NJ; Peppel, KC; Lefkowitz, RJ (Proc Natl Acad Sci U S A, 1996-07-23)
      Guanine nucleotide-binding regulatory protein (G protein)-coupled receptor kinases (GRKs) constitute a family of serine/threonine kinases that play a major role in the agonist-induced phosphorylation and desensitization ...
    • Smoothened signal transduction is promoted by G protein-coupled receptor kinase 2. 

      Meloni, AR; Fralish, GB; Kelly, P; Salahpour, A; Chen, JK; Wechsler Reya, RJ; Lefkowitz, RJ; ... (8 authors) (Mol Cell Biol, 2006-10)
      Deregulation of the Sonic hedgehog pathway has been implicated in an increasing number of human cancers. In this pathway, the seven-transmembrane (7TM) signaling protein Smoothened regulates cellular proliferation ...
    • The kinase Grk2 regulates Nedd4/Nedd4-2-dependent control of epithelial Na+ channels. 

      Dinudom, Anuwat; Fotia, Andrew B; Lefkowitz, Robert J; Young, John A; Kumar, Sharad; Cook, David I (Proc Natl Acad Sci U S A, 2004-08-10)
      Epithelial Na(+) channels mediate the transport of Na across epithelia in the kidney, gut, and lungs and are required for blood pressure regulation. They are inhibited by ubiquitin protein ligases, such as Nedd4 and Nedd4-2, ...
    • Trafficking of G protein-coupled receptors. 

      Drake, Matthew T; Shenoy, Sudha K; Lefkowitz, Robert J (Circ Res, 2006-09-15)
      G protein-coupled receptors (GPCRs) play an integral role in the signal transduction of an enormous array of biological phenomena, thereby serving to modulate at a molecular level almost all components of human biology. ...
    • Visualization of arrestin recruitment by a G-protein-coupled receptor. 

      Shukla, Arun K; Westfield, Gerwin H; Xiao, Kunhong; Reis, Rosana I; Huang, Li-Yin; Tripathi-Shukla, Prachi; Qian, Jiang; ... (29 authors) (Nature, 2014-08-14)
      G-protein-coupled receptors (GPCRs) are critically regulated by β-arrestins, which not only desensitize G-protein signalling but also initiate a G-protein-independent wave of signalling. A recent surge of structural data ...
    • When 7 transmembrane receptors are not G protein-coupled receptors. 

      Rajagopal, Keshava; Lefkowitz, Robert J; Rockman, Howard A (J Clin Invest, 2005-11)
      Classically, 7 transmembrane receptors transduce extracellular signals by coupling to heterotrimeric G proteins, although recent in vitro studies have clearly demonstrated that they can also signal via G protein-independent ...