Browsing by Subject "Saccharomyces cerevisiae"
Now showing items 1-20 of 33
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A new SOD mimic, Mn(III) ortho N-butoxyethylpyridylporphyrin, combines superb potency and lipophilicity with low toxicity.
(Free radical biology & medicine, 2012-05)The Mn porphyrins of k(cat)(O(2)(.-)) as high as that of a superoxide dismutase enzyme and of optimized lipophilicity have already been synthesized. Their exceptional in vivo potency is at least in part due to their ability ... -
A nucleosome-guided map of transcription factor binding sites in yeast.
(PLoS Comput Biol, 2007-11)Finding functional DNA binding sites of transcription factors (TFs) throughout the genome is a crucial step in understanding transcriptional regulation. Unfortunately, these binding sites are typically short and degenerate, ... -
Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro.
(J Cell Biol, 1996-11)In S. cerevisiae lacking SHR3, amino acid permeases specifically accumulate in membranes of the endoplasmic reticulum (ER) and fail to be transported to the plasma membrane. We examined the requirements of transport of the ... -
Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites.
(EMBO J, 2010-09-01)Myosins-I are conserved proteins that bear an N-terminal motor head followed by a Tail Homology 1 (TH1) lipid-binding domain. Some myosins-I have an additional C-terminal extension (C(ext)) that promotes Arp2/3 complex-dependent ... -
Cationic amphiphilic Zn-porphyrin with high antifungal photodynamic potency.
(Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 2017-11)Photodynamic therapy (PDT) is a promising alternative approach particularly attractive for treatment of localized fungal infections. It is based on compounds, photosensitizers (PSs), which when excited with visible light, ... -
Cell-cycle control of cell polarity in yeast.
(The Journal of cell biology, 2019-01)In many cells, morphogenetic events are coordinated with the cell cycle by cyclin-dependent kinases (CDKs). For example, many mammalian cells display extended morphologies during interphase but round up into more spherical ... -
Characterizing the Relationship Between Cell-Cycle Progression and a Transcriptional Oscillator
(2013)The cell division cycle is the process in which the entirety of a cell's contents is duplicated completely and then equally segregated into two identical daughter cells. The order of the steps in the cell cycle must be followed ... -
Chemotactic movement of a polarity site enables yeast cells to find their mates.
(Proceedings of the National Academy of Sciences of the United States of America, 2021-06)How small eukaryotic cells can interpret dynamic, noisy, and spatially complex chemical gradients to orient growth or movement is poorly understood. We address this question using Saccharomyces cerevisiae, where cells orient ... -
Clathrin is important for normal actin dynamics and progression of Sla2p-containing patches during endocytosis in yeast.
(Traffic (Copenhagen, Denmark), 2006-05)Clathrin is a major vesicle coat protein involved in receptor-mediated endocytosis. In yeast and higher eukaryotes, clathrin is recruited to the plasma membrane during the early stage of endocytosis along with clathrin-associated ... -
Conservation, duplication, and loss of the Tor signaling pathway in the fungal kingdom.
(BMC Genomics, 2010-09-23)BACKGROUND: The nutrient-sensing Tor pathway governs cell growth and is conserved in nearly all eukaryotic organisms from unicellular yeasts to multicellular organisms, including humans. Tor is the target of the immunosuppressive ... -
Cytocidal amino acid starvation of Saccharomyces cerevisiae and Candida albicans acetolactate synthase (ilv2{Delta}) mutants is influenced by the carbon source and rapamycin.
(Microbiology, 2010-03)The isoleucine and valine biosynthetic enzyme acetolactate synthase (Ilv2p) is an attractive antifungal drug target, since the isoleucine and valine biosynthetic pathway is not present in mammals, Saccharomyces cerevisiae ... -
Detecting separate time scales in genetic expression data.
(BMC Genomics, 2010-06-16)BACKGROUND: Biological processes occur on a vast range of time scales, and many of them occur concurrently. As a result, system-wide measurements of gene expression have the potential to capture many of these processes ... -
Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast.
(Mol Cell Biol, 2015-11)Cells respond to environmental stimuli by fine-tuned regulation of gene expression. Here we investigated the dose-dependent modulation of gene expression at high temporal resolution in response to nutrient and stress signals ... -
Environmental and genetic determinants of colony morphology in yeast.
(PLoS Genet, 2010-01-22)Nutrient stresses trigger a variety of developmental switches in the budding yeast Saccharomyces cerevisiae. One of the least understood of such responses is the development of complex colony morphology, characterized by ... -
Evolutionary Implications and Genetic Basis of Peroxide Survival in Saccharomyces Cerevisiae
(2009)Hydrogen peroxide is used by animals and plants to deter the growth of microbial invaders by inflicting DNA lesions, protein oxidation and lipid membrane modifications. Pathogens protect themselves with enzymes and scavenging ... -
Exploratory polarization facilitates mating partner selection in Saccharomyces cerevisiae.
(Molecular biology of the cell, 2021-05)Yeast decode pheromone gradients to locate mating partners, providing a model for chemotropism. How yeast polarize toward a single partner in crowded environments is unclear. Initially, cells often polarize in unproductive ... -
Ferrochelatase is a conserved downstream target of the blue light-sensing White collar complex in fungi.
(Microbiology, 2010-08)Light is a universal signal perceived by organisms, including fungi, in which light regulates common and unique biological processes depending on the species. Previous research has established that conserved proteins, originally ... -
How cells determine the number of polarity sites.
(eLife, 2021-04-26)The diversity of cell morphologies arises, in part, through regulation of cell polarity by Rho-family GTPases. A poorly understood but fundamental question concerns the regulatory mechanisms by which different cells generate ... -
In vivo dynamics of clathrin and its adaptor-dependent recruitment to the actin-based endocytic machinery in yeast.
(Developmental cell, 2005-07)Clathrin-mediated transport is a major pathway for endocytosis. However, in yeast, where cortical actin patches are essential for endocytosis, plasma membrane-associated clathrin has never been observed. Using live cell ... -
Investigating Transcription Factor Networks That Drive Biological Clocks and Oscillators
(2017)Biological systems are highly dynamic, yet our temporal resolution of suchdynamical processes is often limited or difficult to test in the laboratory. The 24-hourcircadian rhythm and the approximately 75-minute cell cycle ...
