Browsing by Subject "Saccharomyces cerevisiae Proteins"
Now showing items 1-20 of 20
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A nucleosome-guided map of transcription factor binding sites in yeast.
(PLoS Comput Biol, 2007-11)Finding functional DNA binding sites of transcription factors (TFs) throughout the genome is a crucial step in understanding transcriptional regulation. Unfortunately, these binding sites are typically short and degenerate, ... -
Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro.
(J Cell Biol, 1996-11)In S. cerevisiae lacking SHR3, amino acid permeases specifically accumulate in membranes of the endoplasmic reticulum (ER) and fail to be transported to the plasma membrane. We examined the requirements of transport of the ... -
Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites.
(EMBO J, 2010-09-01)Myosins-I are conserved proteins that bear an N-terminal motor head followed by a Tail Homology 1 (TH1) lipid-binding domain. Some myosins-I have an additional C-terminal extension (C(ext)) that promotes Arp2/3 complex-dependent ... -
Cell-cycle control of cell polarity in yeast.
(The Journal of cell biology, 2019-01)In many cells, morphogenetic events are coordinated with the cell cycle by cyclin-dependent kinases (CDKs). For example, many mammalian cells display extended morphologies during interphase but round up into more spherical ... -
Chemotactic movement of a polarity site enables yeast cells to find their mates.
(Proceedings of the National Academy of Sciences of the United States of America, 2021-06)How small eukaryotic cells can interpret dynamic, noisy, and spatially complex chemical gradients to orient growth or movement is poorly understood. We address this question using Saccharomyces cerevisiae, where cells orient ... -
Chromatin: bind at your own RSC.
(Curr Biol, 2011-03-22)Recent work has identified a novel RSC-nucleosome complex that both strongly phases flanking nucleosomes and presents regulatory sites for ready access. These results challenge several widely held views. -
Clathrin is important for normal actin dynamics and progression of Sla2p-containing patches during endocytosis in yeast.
(Traffic (Copenhagen, Denmark), 2006-05)Clathrin is a major vesicle coat protein involved in receptor-mediated endocytosis. In yeast and higher eukaryotes, clathrin is recruited to the plasma membrane during the early stage of endocytosis along with clathrin-associated ... -
Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast.
(Mol Cell Biol, 2015-11)Cells respond to environmental stimuli by fine-tuned regulation of gene expression. Here we investigated the dose-dependent modulation of gene expression at high temporal resolution in response to nutrient and stress signals ... -
DNA mismatches reveal conformational penalties in protein-DNA recognition.
(Nature, 2020-11)Transcription factors recognize specific genomic sequences to regulate complex gene-expression programs. Although it is well-established that transcription factors bind to specific DNA sequences using a combination of base ... -
Environmental and genetic determinants of colony morphology in yeast.
(PLoS Genet, 2010-01-22)Nutrient stresses trigger a variety of developmental switches in the budding yeast Saccharomyces cerevisiae. One of the least understood of such responses is the development of complex colony morphology, characterized by ... -
How cells determine the number of polarity sites.
(eLife, 2021-04-26)The diversity of cell morphologies arises, in part, through regulation of cell polarity by Rho-family GTPases. A poorly understood but fundamental question concerns the regulatory mechanisms by which different cells generate ... -
How the kinetochore couples microtubule force and centromere stretch to move chromosomes.
(Nature cell biology, 2016-04)The Ndc80 complex (Ndc80, Nuf2, Spc24 and Spc25) is a highly conserved kinetochore protein essential for end-on anchorage to spindle microtubule plus ends and for force generation coupled to plus-end polymerization ... -
In vivo dynamics of clathrin and its adaptor-dependent recruitment to the actin-based endocytic machinery in yeast.
(Developmental cell, 2005-07)Clathrin-mediated transport is a major pathway for endocytosis. However, in yeast, where cortical actin patches are essential for endocytosis, plasma membrane-associated clathrin has never been observed. Using live cell ... -
Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy.
(Mol Biol Cell, 2014-11-05)Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence ... -
Mechanisms that ensure monogamous mating in Saccharomyces cerevisiae.
(Molecular biology of the cell, 2021-04)Haploid cells of the budding yeast Saccharomyces cerevisiae communicate using secreted pheromones and mate to form diploid zygotes. Mating is monogamous, resulting in the fusion of precisely one cell of each mating type. ... -
Novel function of clathrin light chain in promoting endocytic vesicle formation.
(Molecular biology of the cell, 2006-10)Clathrin-mediated endocytosis is a major pathway for uptake of lipid and protein cargo at the plasma membrane. The lattices of clathrin-coated pits and vesicles are comprised of triskelions, each consisting of three oligomerized ... -
Ratiometric GPCR signaling enables directional sensing in yeast.
(PLoS biology, 2019-10-17)Accurate detection of extracellular chemical gradients is essential for many cellular behaviors. Gradient sensing is challenging for small cells, which can experience little difference in ligand concentrations on the up-gradient ... -
Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis.
(J Cell Sci, 2012-10-15)Phosphorylation regulates assembly and disassembly of proteins during endocytosis. In yeast, Prk1 and Ark1 phosphorylate factors after vesicle internalization leading to coat disassembly. Scd5, a protein phosphatase-1 ... -
Stability selection for regression-based models of transcription factor-DNA binding specificity.
(Bioinformatics, 2013-07-01)MOTIVATION: The DNA binding specificity of a transcription factor (TF) is typically represented using a position weight matrix model, which implicitly assumes that individual bases in a TF binding site contribute independently ... -
The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe.
(PLoS One, 2010-12-20)A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, ...
