Browsing by Subject "Staining and Labeling"
Now showing items 1-5 of 5
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A chemical method for labeling lysine methyltransferase substrates.
(Chembiochem : a European journal of chemical biology, 2011-01)Several protein lysine methyltransferases (PKMTs) modify histones to regulate chromatin-dependent cellular processes, such as transcription, DNA replication and DNA damage repair. PKMTs are likely to have many additional ... -
Biomimetic nanoparticles with enhanced affinity towards activated endothelium as versatile tools for theranostic drug delivery.
(Theranostics, 2018-01-05)Activation of the vascular endothelium is characterized by increased expression of vascular adhesion molecules and chemokines. This activation occurs early in the progression of several diseases and triggers the recruitment ... -
Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners.
(Proceedings of the National Academy of Sciences of the United States of America, 2012-03-12)O-linked β-N-acetylglucosamine (O-GlcNAc) is a reversible posttranslational modification found on hundreds of nuclear and cytoplasmic proteins in higher eukaryotes. Despite its ubiquity and essentiality in mammals, functional ... -
Robust approaches to quantitative ratiometric FRET imaging of CFP/YFP fluorophores under confocal microscopy.
(J Microsc, 2009-01)Ratiometric quantification of CFP/YFP FRET enables live-cell time-series detection of molecular interactions, without the need for acceptor photobleaching or specialized equipment for determining fluorescence lifetime. Although ... -
Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling.
(J Vis Exp, 2015-09-14)Transsynaptic tracing has become a powerful tool used to analyze central efferents that regulate peripheral targets through multi-synaptic circuits. This approach has been most extensively used in the brain by utilizing ...
