Browsing by Subject "Tissue Engineering"
Now showing items 1-18 of 18
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A Tissue-Engineered Microvascular System to Evaluate Vascular Progenitor Cells for Angiogenic Therapies
(2015)The ability of tissue engineered constructs to replace diseased or damaged organs is limited without the incorporation of a functional vascular system. To design microvasculature that recapitulates the vascular niche functions ... -
Advanced Fibrous Scaffold Engineering for Controlled Delivery and Regenerative Medicine Applications
(2010)Continuous nanostructures, such as electrospun nanofibers, embedded with proteins may synergistically present the topographical and biochemical signals to cells for tissue engineering applications. In this dissertation, ... -
Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo.
(Proc Natl Acad Sci U S A, 2014-04-15)Tissue-engineered skeletal muscle can serve as a physiological model of natural muscle and a potential therapeutic vehicle for rapid repair of severe muscle loss and injury. Here, we describe a platform for engineering and ... -
Biomimetic Poly(ethylene glycol)-based Hydrogels as a 3D Tumor Model for Evaluation of Tumor Stromal Cell and Matrix Influences on Tissue Vascularization
(2015)To this day, cancer remains the leading cause of mortality worldwide1. A major contributor to cancer progression and metastasis is tumor angiogenesis. The formation of blood vessels around a tumor is facilitated by the complex ... -
Cellular and Biomaterial Engineering for Orthopaedic Regenerative Medicine
(2015)The ends of long bones that articulate with respect to one another are lined with a crucial connective tissue called articular cartilage. This tissue plays an essential biomechanical function in synovial joints, as it serves ... -
Chondrogenesis and mineralization during in vitro culture of human mesenchymal stem cells on three-dimensional woven scaffolds.
(Tissue Eng Part A, 2010-12)Human mesenchymal stem cells (hMSCs) and three-dimensional (3D) woven poly(ɛ-caprolactone) (PCL) scaffolds are promising tools for skeletal tissue engineering. We hypothesized that in vitro culture duration and medium additives ... -
Effect of microRNA modulation on bioartificial muscle function.
(Tissue Eng Part A, 2010-12)Cellular therapies have recently employed the use of small RNA molecules, particularly microRNAs (miRNAs), to regulate various cellular processes that may be altered in disease states. In this study, we examined the effect ... -
Electrospun Scaffolds for Cartilage Tissue Engineering: Methods to Affect Anisotropy, Material and Cellular Infiltration
(2011)The aim of this dissertation was to develop new techniques for producing electrospun scaffolds for use in the tissue engineering of articular cartilage. We developed a novel method of imparting mechanical anisotropy to ... -
Functional properties of cell-seeded three-dimensionally woven poly(epsilon-caprolactone) scaffolds for cartilage tissue engineering.
(Tissue Eng Part A, 2010-04)Articular cartilage possesses complex mechanical properties that provide healthy joints the ability to bear repeated loads and maintain smooth articulating surfaces over an entire lifetime. In this study, we utilized ... -
Induced pluripotent stem cell-derived cardiac progenitors differentiate to cardiomyocytes and form biosynthetic tissues.
(PLoS One, 2013)The mammalian heart has little capacity to regenerate, and following injury the myocardium is replaced by non-contractile scar tissue. Consequently, increased wall stress and workload on the remaining myocardium leads to ... -
Mechanical regulation of chondrogenesis.
(Stem Cell Res Ther, 2013-07-01)Mechanical factors play a crucial role in the development of articular cartilage in vivo. In this regard, tissue engineers have sought to leverage native mechanotransduction pathways to enhance in vitro stem cell-based cartilage ... -
Neural network analysis identifies scaffold properties necessary for in vitro chondrogenesis in elastin-like polypeptide biopolymer scaffolds.
(Tissue Eng Part A, 2010-01)The successful design of biomaterial scaffolds for articular cartilage tissue engineering requires an understanding of the impact of combinations of material formulation parameters on diverse and competing functional outcomes ... -
Order and Disorder in Protein Biomaterial Design
(2018)Crystalline and amorphous materials have been extensively studied for their interesting properties, but they comprise a very small portion of the total materials space. The properties of most materials are a consequence ... -
Porcine endothelial cells cocultured with smooth muscle cells became procoagulant in vitro.
(Tissue Eng Part A, 2010-06)Endothelial cell (EC) seeding represents a promising approach to provide a nonthrombogenic surface on vascular grafts. In this study, we used a porcine EC/smooth muscle cell (SMC) coculture model that was previously developed ... -
Role of Non-myocytes in Engineering of Highly Functional Pluripotent Stem Cell-derived Cardiac Tissues
(2013)Massive loss of cardiac tissue as a result of myocardial infarction can create a poorly-conducting substrate with impaired contractility, ultimately leading to heart failure and lethal arrhythmias. Recent advances in pluripotent ... -
Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels.
(Sci Rep, 2015-10-12)Tissue-engineered blood vessels (TEBV) can serve as vascular grafts and may also play an important role in the development of organs-on-a-chip. Most TEBV construction involves scaffolding with biomaterials such as collagen ... -
Tissue-engineered cardiac patch for advanced functional maturation of human ESC-derived cardiomyocytes.
(Biomaterials, 2013-07)Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) provide a promising source for cell therapy and drug screening. Several high-yield protocols exist for hESC-CM production; however, methods to significantly advance ... -
Utility of telomerase-pot1 fusion protein in vascular tissue engineering.
(Cell Transplant, 2010)While advances in regenerative medicine and vascular tissue engineering have been substantial in recent years, important stumbling blocks remain. In particular, the limited life span of differentiated cells that are harvested ...
