Browsing by Subject "X-ray crystallography"
Now showing items 1-8 of 8
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Characterization of Peripheral-Membrane Enzymes Required for Lipid A Biosynthesis in Gram-Negative Bacteria
(2010)Gram-negative bacteria possess an asymmetric outer membrane in which the inner leaflet is composed primarily of phospholipids while the outer leaflet contains both phospholipids and lipopolysaccharide (LPS). LPS forms a ... -
Conformational Heterogeneity of a Multifunctional Protein
(2015)The structural plasticity conferred by conformational flexibility has increasingly been recognized as a likely determinant of function. For example, multiscale heterogeneity in the calmodulin central helix most likely helps ... -
Structural and Biochemical Analyses of the Francisella tularensis Virulence Regulators MglA, SspA and PigR
(2017)Francisella tularensis is one of the most infectious bacteria known and is the etiologic agent of tularemia. Francisella virulence arises from a 33 kilobase pathogenicity island (FPI) that is regulated by the macrophage ... -
Structural and Kinetic Characterization of LpxK, the Tetraacyldisaccharide-1- Phosphate Kinase of Lipid A Biosynthesis
(2013)Lipopolysaccharide, the physical barrier that protects Gram-negative bacteria from various antibiotics and environmental stressors, is anchored to the outer membrane by the phosphorylated, acylated disaccharide of glucosamine ... -
Structural Characterization of the Bacterial Riboregulator Hfq and the Novel M. tuberculosis Toxin-Antitoxin Module Rv3188-Rv3189
(2017)The bacterial protein Hfq is an RNA chaperone and pleiotropic posttranscriptional regulator. Hfq binds to A and U-‐‑rich regions of small regulatory RNA (sRNA) to their cognate mRNA to facilitate their annealing, affecting ... -
Structural Studies on the Lipid Flippase MurJ
(2018)The biosynthesis of many important polysaccharides (including peptidoglycan, lipopolysaccharide, and N-linked glycans) necessitates membrane transport of oligosaccharide precursors from their cytoplasmic site of synthesis ... -
Structure-Guided Design of Novel Therapeutics Targeting Translesion DNA Synthesis and Lipid A Biosynthesis
(2019)Cancer is one of the most devastating diseases in modern society, with over 1.6 million new cancer cases occurring in the US alone each year. DNA-damaging agents are often the first line of defense against rapidly dividing ... -
Suppression of conformational heterogeneity at a protein-protein interface.
(Proc Natl Acad Sci U S A, 2015-07-21)Staphylococcal protein A (SpA) is an important virulence factor from Staphylococcus aureus responsible for the bacterium's evasion of the host immune system. SpA includes five small three-helix-bundle domains that can each ...
