Skip to main content
Duke University Libraries
DukeSpace Scholarship by Duke Authors
  • Login
  • Ask
  • Menu
  • Login
  • Ask a Librarian
  • Search & Find
  • Using the Library
  • Research Support
  • Course Support
  • Libraries
  • About
View Item 
  •   DukeSpace
  • Theses and Dissertations
  • Masters Theses
  • View Item
  •   DukeSpace
  • Theses and Dissertations
  • Masters Theses
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Discovery of potent inhibitors of Plasmodium PK5

Thumbnail
View / Download
1.1 Mb
Date
2015
Author
Perkins, Marisha Marie
Advisor
Derbyshire, Emily Rose
Repository Usage Stats
299
views
145
downloads
Abstract

Malaria is one of the oldest and deadliest diseases in the world, affecting approximately 200 million people annually. The role of protein phosphorylation in the complex life cycle of the malaria parasite, Plasmodium, as well as the promising therapeutic values of protein kinase inhibitors have sparked increasing interest in understanding the Plasmodium kinome. Although many protein kinases have been shown to be essential for Plasmodium survival, their functions remain unknown. Protein kinase 5 (PK5) is a putative cyclin dependent kinase (CDK)-like protein in the malaria parasite, and it is thought to be essential for blood stage proliferation in P. falciparum. In the present study, biochemical binding assays were used to identify potent and selective inhibitors of PfPK5. Two compounds were found to selectively bind to PfPK5 over the human analog, Homo sapiens CDK2. In addition, a known CDK inhibitor was used in the development of a chemical probe to identify potential macromolecules essential to parasite survival. Here, we report important structural moieties potentially involved in PfPK5 binding. Elucidation of the biological targets through the use of our chemical probe may aid in further understanding of Plasmodium biology.

Type
Master's thesis
Department
Chemistry
Subject
Chemistry
Competition Binding Assay
Phenotypic Assay
Plasmodium
Protein Kinase
Permalink
https://hdl.handle.net/10161/10041
Citation
Perkins, Marisha Marie (2015). Discovery of potent inhibitors of Plasmodium PK5. Master's thesis, Duke University. Retrieved from https://hdl.handle.net/10161/10041.
Collections
  • Masters Theses
More Info
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

Rights for Collection: Masters Theses


Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info

Make Your Work Available Here

How to Deposit

Browse

All of DukeSpaceCommunities & CollectionsAuthorsTitlesTypesBy Issue DateDepartmentsAffiliations of Duke Author(s)SubjectsBy Submit DateThis CollectionAuthorsTitlesTypesBy Issue DateDepartmentsAffiliations of Duke Author(s)SubjectsBy Submit Date

My Account

LoginRegister

Statistics

View Usage Statistics
Duke University Libraries

Contact Us

411 Chapel Drive
Durham, NC 27708
(919) 660-5870
Perkins Library Service Desk

Digital Repositories at Duke

  • Report a problem with the repositories
  • About digital repositories at Duke
  • Accessibility Policy
  • Deaccession and DMCA Takedown Policy

TwitterFacebookYouTubeFlickrInstagramBlogs

Sign Up for Our Newsletter
  • Re-use & Attribution / Privacy
  • Harmful Language Statement
  • Support the Libraries
Duke University