Show simple item record

Magnetic resonance imaging of graded skeletal muscle injury in live rats.

dc.contributor.author Cutlip, Robert G
dc.contributor.author Hollander, Melinda S
dc.contributor.author Johnson, G Allan
dc.contributor.author Johnson, Brice W
dc.contributor.author Friend, Sherri A
dc.contributor.author Baker, Brent A
dc.coverage.spatial United States
dc.date.accessioned 2015-07-16T15:02:09Z
dc.date.issued 2014
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/25525369
dc.identifier ehi-suppl.1-2014-031
dc.identifier.uri https://hdl.handle.net/10161/10309
dc.description.abstract INTRODUCTION: Increasing number of stretch-shortening contractions (SSCs) results in increased muscle injury. METHODS: Fischer Hybrid rats were acutely exposed to an increasing number of SSCs in vivo using a custom-designed dynamometer. Magnetic resonance imaging (MRI) imaging was conducted 72 hours after exposure when rats were infused with Prohance and imaged using a 7T rodent MRI system (GE Epic 12.0). Images were acquired in the transverse plane with typically 60 total slices acquired covering the entire length of the hind legs. Rats were euthanized after MRI, the lower limbs removed, and tibialis anterior muscles were prepared for histology and quantified stereology. RESULTS: Stereological analyses showed myofiber degeneration, and cellular infiltrates significantly increased following 70 and 150 SSC exposure compared to controls. MRI images revealed that the percent affected area significantly increased with exposure in all SSC groups in a graded fashion. Signal intensity also significantly increased with increasing SSC repetitions. DISCUSSION: These results suggest that contrast-enhanced MRI has the sensitivity to differentiate specific degrees of skeletal muscle strain injury, and imaging data are specifically representative of cellular histopathology quantified via stereological analyses.
dc.language eng
dc.publisher SAGE Publications
dc.relation.ispartof Environ Health Insights
dc.relation.isversionof 10.4137/EHI.S15255
dc.subject MRI
dc.subject inflammation
dc.subject myofiber degeneration
dc.subject skeletal muscle injury
dc.subject stretch–shortening contraction
dc.title Magnetic resonance imaging of graded skeletal muscle injury in live rats.
dc.type Journal article
duke.contributor.id Johnson, G Allan|0079419
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/25525369
pubs.begin-page 31
pubs.end-page 39
pubs.issue Suppl 1
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Physics
pubs.organisational-group Radiology
pubs.organisational-group School of Medicine
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published online
pubs.volume 8


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record