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Localization of Metal Electrodes in the Intact Rat Brain Using Registration of 3D Microcomputed Tomography Images to a Magnetic Resonance Histology Atlas.

dc.contributor.author Badea, Alexandra
dc.contributor.author Badea, Cristian Tudorel
dc.contributor.author Borg, JS
dc.contributor.author Dzirasa, Kafui
dc.contributor.author Johnson, G Allan
dc.contributor.author Vu, MA
dc.coverage.spatial United States
dc.date.accessioned 2015-07-28T15:10:19Z
dc.date.issued 2015-07
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/26322331
dc.identifier.uri https://hdl.handle.net/10161/10327
dc.description.abstract Simultaneous neural recordings taken from multiple areas of the rodent brain are garnering growing interest due to the insight they can provide about spatially distributed neural circuitry. The promise of such recordings has inspired great progress in methods for surgically implanting large numbers of metal electrodes into intact rodent brains. However, methods for localizing the precise location of these electrodes have remained severely lacking. Traditional histological techniques that require slicing and staining of physical brain tissue are cumbersome, and become increasingly impractical as the number of implanted electrodes increases. Here we solve these problems by describing a method that registers 3-D computerized tomography (CT) images of intact rat brains implanted with metal electrode bundles to a Magnetic Resonance Imaging Histology (MRH) Atlas. Our method allows accurate visualization of each electrode bundle's trajectory and location without removing the electrodes from the brain or surgically implanting external markers. In addition, unlike physical brain slices, once the 3D images of the electrode bundles and the MRH atlas are registered, it is possible to verify electrode placements from many angles by "re-slicing" the images along different planes of view. Further, our method can be fully automated and easily scaled to applications with large numbers of specimens. Our digital imaging approach to efficiently localizing metal electrodes offers a substantial addition to currently available methods, which, in turn, may help accelerate the rate at which insights are gleaned from rodent network neuroscience.
dc.language eng
dc.relation.ispartof eNeuro
dc.relation.isversionof 10.1523/ENEURO.0017-15.2015
dc.title Localization of Metal Electrodes in the Intact Rat Brain Using Registration of 3D Microcomputed Tomography Images to a Magnetic Resonance Histology Atlas.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/26322331
pubs.issue 4
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Neurobiology
pubs.organisational-group Neurosurgery
pubs.organisational-group Physics
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group Psychiatry & Behavioral Sciences
pubs.organisational-group Psychiatry & Behavioral Sciences, Brain Stimulation and Neurophysiology
pubs.organisational-group Radiology
pubs.organisational-group School of Medicine
pubs.organisational-group Trinity College of Arts & Sciences
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 2
dc.identifier.eissn 2373-2822


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