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Quantitative mapping of trimethyltin injury in the rat brain using magnetic resonance histology.

dc.contributor.author Badea, Alexandra
dc.contributor.author Calabrese, Evan
dc.contributor.author Hedlund, Laurence W
dc.contributor.author Johnson, G Allan
dc.contributor.author Little, PB
dc.contributor.author Qi, Y
dc.coverage.spatial Netherlands
dc.date.accessioned 2015-07-28T15:27:30Z
dc.date.issued 2014-05
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/24631313
dc.identifier S0161-813X(14)00032-1
dc.identifier.uri http://hdl.handle.net/10161/10329
dc.description.abstract The growing exposure to chemicals in our environment and the increasing concern over their impact on health have elevated the need for new methods for surveying the detrimental effects of these compounds. Today's gold standard for assessing the effects of toxicants on the brain is based on hematoxylin and eosin (H&E)-stained histology, sometimes accompanied by special stains or immunohistochemistry for neural processes and myelin. This approach is time-consuming and is usually limited to a fraction of the total brain volume. We demonstrate that magnetic resonance histology (MRH) can be used for quantitatively assessing the effects of central nervous system toxicants in rat models. We show that subtle and sparse changes to brain structure can be detected using magnetic resonance histology, and correspond to some of the locations in which lesions are found by traditional pathological examination. We report for the first time diffusion tensor image-based detection of changes in white matter regions, including fimbria and corpus callosum, in the brains of rats exposed to 8 mg/kg and 12 mg/kg trimethyltin. Besides detecting brain-wide changes, magnetic resonance histology provides a quantitative assessment of dose-dependent effects. These effects can be found in different magnetic resonance contrast mechanisms, providing multivariate biomarkers for the same spatial location. In this study, deformation-based morphometry detected areas where previous studies have detected cell loss, while voxel-wise analyses of diffusion tensor parameters revealed microstructural changes due to such things as cellular swelling, apoptosis, and inflammation. Magnetic resonance histology brings a valuable addition to pathology with the ability to generate brain-wide quantitative parametric maps for markers of toxic insults in the rodent brain.
dc.language eng
dc.relation.ispartof Neurotoxicology
dc.relation.isversionof 10.1016/j.neuro.2014.02.009
dc.subject Animal models
dc.subject Environmental toxins
dc.subject MRI
dc.subject Rat
dc.subject Trimethyltin
dc.subject Animals
dc.subject Diffusion Magnetic Resonance Imaging
dc.subject Diffusion Tensor Imaging
dc.subject Dose-Response Relationship, Drug
dc.subject Environmental Pollutants
dc.subject Histological Techniques
dc.subject Male
dc.subject Rats
dc.subject Trimethyltin Compounds
dc.subject White Matter
dc.title Quantitative mapping of trimethyltin injury in the rat brain using magnetic resonance histology.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/24631313
pubs.begin-page 12
pubs.end-page 23
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Physics
pubs.organisational-group Radiology
pubs.organisational-group School of Medicine
pubs.organisational-group Staff
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published
pubs.volume 42
dc.identifier.eissn 1872-9711


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